UK scientists have developed a fast and sensitive method for screening transaminase activity and enantioselectivity, using D- and L-amino acid oxidases, allowing new amine substrates to be rapidly identified.
Nicholas Turner and colleagues from the University of Manchester and Richard Lloyd from Chirotech Technology Ltd in Cambridge, use inexpensive and readily available reagents. Moreover the technique only requires a UV/Vis-plate reader to operate in 96-well microtitre plate format. The team plan to develop this assay further and hopefully use it for high-throughput screening of transaminase libraries.
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