RSC Advances Blog

We are very pleased to introduce Michael Eze, the corresponding author of the paper ‘Ethanol-blended petroleum fuels: implications of co-solvency for phytotechnologies‘. His article has been very well received and handpicked by our reviewers and handling editors as one of our February HOT articles. Michael was kind enough to tell us more about the work that went into this article and what he hopes to achieve in the future. You can find out more about the author and their article below and find more HOT articles in our online collection.

Meet the Author

Michael Onyedika Eze is a third year Cotutelle PhD student between Macquarie University, Sydney, Australia and Georg-August University, Goettingen, Germany. For this dual doctoral research, he was awarded the Australian Commonwealth scholarship, the Macquarie University Cotutelle Research Excellence scholarship and the prestigious German Academic Exchange Service (DAAD) scholarship. Prior to starting his PhD, he obtained a Bachelor of Science (Hons) degree in Pure and Industrial Chemistry from the University of Nigeria, Nsukka, and a Master’s degree in Analytical Chemistry. Michael is a passionate science communicator, receiving a 1^st Place Award in a Three-Minute Thesis (3MT) Competition, as well as being a Finalist in a Falling Walls Lab Competition. He is also a recipient of various travel and research grants including the Royal Society of Chemistry competitive travel grants for PhD students and early career researchers, the European Association of Geochemistry travel grants, and the Petroleum Exploration Society of Australia 2018 tertiary institution grant, among others. In 2019, he was awarded the Carlos Walter M. Campos Memorial Award by the American Association of Petroleum Geologists (AAPG) for the Best International Student Paper at the 2019 AAPG international conference and exhibition.

Michael’s current research interest focuses on rhizoremediation of organic contaminants. Michael takes an interdisciplinary approach (involving organic geochemistry, geomicrobiology and genomics) and seeks to develop novel plant-microbe symbionts for enhanced rhizoremediation of petroleum contaminated sites. He also aims to fully delineate genes responsible for petroleum degradation and their pathways with the goal of revolutionising the way oil spills and other environmental contaminants are cleaned up.

Picture of the Organic Geochemistry Research Group, Macquarie University, Sydney, Australia. Group Leader: Professor Simon C. George

Could you briefly explain the focus of your article to the non-specialist (in one or two sentences only) and why it is of current interest?
Our article describes the effect that the current practice of adding ethanol to petroleum fuels could have on the effectiveness of phytoremediation as a reclamation strategy for soils contaminated with oil spills.

How big an impact could your results potentially have?
Around the globe, petroleum contamination remains the most persistent environmental problem resulting from oil and gas operations. The United States Environmental Protection Agency (US EPA) estimated that rehabilitation can cost over $US1 million per hectare using traditional methods. Thus, developing an affordable and eco-friendly remediation technique will have global impact.

Could you explain the motivation behind this study?
The motivation for this research stems from a number of reasons. On one hand, natural attenuation exhibit slow metabolic activity. On the other hand, traditional methods of remediation are very expensive and environmentally unfriendly. As a result, a number of contaminated sites are left as is or their rehabilitation is postponed. Suffice to say that the future of humanity hangs in the balance if our assault on “mother nature” does not receive commensurate attention. While microbial-enhanced rhizoremediation may prove to be the panacea, its success relies on the containment of contaminants within the rooting zones of plants. It thus implies that any energy innovation that increases the leaching potentials of hydrocarbons beyond the rhizospheric zones will limit the effectiveness of phytotechnology as a reclamation strategy for oil spills. Since ethanol is a co-solvent, we therefore decided to first examine its effect on the leaching potentials of both aliphatic and aromatic hydrocarbons. That is the crux of this paper.

In your opinion, what are the key design considerations for your study?
Well, owing to the interdisciplinary nature of my research, my PhD programme is domiciled in two different departments and in two separate continents. As a result, I am expected to submit two theses for the awards of PhD in Organic Geochemistry (Macquarie University, Sydney, Australia) and Dr. rer. nat. in Genomics and Applied Microbiology (Georg-August University, Goettingen, Germany). Thus, I needed to make sure from the outset that the entire research with its four separate projects are properly linked together and feasible, while maintaining the overarching aim of the research.

Which part of the work towards this paper proved to be most challenging?
For this particular experiment, setting up the leaching column was something that needed a careful thought. Controlling the two major factors that affect soil total petroleum hydrocarbon content, namely biodegradation and volatilisation while in the column seemed to be challenging. The methodology employed to achieve this is discussed in the paper.

What aspect of your work are you most excited about at the moment?
In view of the extensive use of high throughput sequencing techniques in my study, I am beginning to delineate genes and degradation pathways involved in hydrocarbon-degradation. Gaining insight into the full genomes and metagenomes of plant growth-promoting and hydrocarbon-degrading rhizobacteria is not only exciting but exhilarating!

What is the next step? What work is planned?
Today, the majority of research is still exploring remediation by plant or single microorganisms. What we can expect is more focus on plant-microbiome interactions. Our goal is to develop the right plant-microbe symbionts for effective remediation of petroleum contaminated sites. The results discussed in this article and that from our subsequent projects (phytotoxicity screening and microbial genomics) will guide us towards the achievement of this noble goal. By directing our attention to full genomes of plant-microbe symbionts, we hope to gain a better understanding of the integrated activity of plants and oil-degrading microbes. Armed with this knowledge we will be able to engineer microbes that can degrade hydrocarbon spills even better. Additionally, in the near future, I hope to direct my attention to extremophiles, that is, organisms that maintain optimal growth in environmental conditions considered extreme. This, I hope, will prove useful for environments with mixed contaminants such as those with petroleum and heavy metals/acidic contaminants.

Ethanol-blended petroleum fuels: implications of co-solvency for phytotechnologies
Michael O. Eze and Simon C. George
RSC Adv., 2020, 10, 6473-6481
DOI: 10.1039/C9RA10919F, Paper

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We are very pleased to introduce Dr Selma Leulmi Pichot, the corresponding author of the paper ‘Versatile magnetic microdiscs for the radio enhancement and mechanical disruption of glioblastoma cancer cells‘. Her article has been very well received and handpicked by our reviewers and handling editors as one of our February HOT articles. Selma was kind enough to tell us more about the work that went into this article and what she hopes to achieve in the future. You can find out more about the author and their article below and find more HOT articles in our online collection.

Meet the Author

Selma Pichot completed her undergraduate engineering degree in Biology at the University of Algiers (Algeria), before moving to France for a Research Master in Nanosciences, with a focus in Nanobiotechnologies. After completing a PhD in Physics for life science at the University of Grenoble in 2014, she took up a research associate position with Professor Cowburn at the University of Cambridge to work on the use of magnetic micro and nanostructures for biological and biomedical applications.

Could you briefly explain the focus of your article to the non-specialist (in one or two sentences only) and why it is of current interest?
Our article describes the use of a new type of magnetic microparticle to improve the treatment of glioblastoma multiforme, the most aggressive form of brain cancer.

How big an impact could your results potentially have?
Our results demonstrate that we have powerful tools that can efficiently contribute to improve the current treatment of glioblastoma. Moreover, when used as an adjunct to surgery, the magnetic microdiscs have the potential to shorten the course of radiation therapy in countries where access to radiation therapy is scarce.

Could you explain the motivation behind this study?
Glioblastoma is the most aggressive form of brain cancer, with a median survival for diagnosed patients being 12-15 months with actual treatment modalities. With such a terrible prognosis, we believe current advances in magnetism and nanotechnology could provide new insights to open up the current bottlenecks in the treatment of this devastating disease.

In your opinion, what are the key design considerations for your study?
To conduct such a study, we must first have microdiscs that have perfectly controlled magnetic behavior. This includes the fact that they do not agglomerate in liquid, and that they can deliver significant forces when activated by a magnetic field, in order to destabilize the internal structures of cancer cells. Another important element is the fact that the microdiscs must be able to reach a suitable cellular location to enhance the effect of the radiotherapy very locally, at the intracellular level.

Which part of the work towards this paper proved to be most challenging?
The first challenging aspect of this work is to construct high quality magnetic microdiscs with the specific desired properties. The microdiscs are fabricated by the deposition of ultra thin films of alternating layers of magnetic and non magnetic materials. Inspired by processes often used in the manufacture of MRAM magnetic memory devices, we control these film thicknesses with sub-nanometre resolution. Another important part of the work was in the optimisation of the experimental parameters for the in vitro testing with the cancer cells to ensure that our testing methodology was robust.

What aspect of your work are you most excited about at the moment?
At the moment, I am very excited in using cutting edge physics to solve current challenges in areas like cancer biology and more recently, in microbiology. There are also a variety of new directions that would be very interesting to explore in the field of neurobiology and the treatment of neurodegenerative diseases for example.

What is the next step? What work is planned?
Of course after such positive results, we would like to take this work a step further. Now that we have a good proof of concept on our initial hypothesis, we would like to translate our results into a realistic in vitro model. This includes for example the use of patient derived cancer cells to construct 3D structures that structurally and functionally mimics the glioblastoma tumoral cavity. A very important aspect is to continue working closely with clinicians to translate this research into clinic.

Versatile magnetic microdiscs for the radio enhancement and mechanical disruption of glioblastoma cancer cells
Selma Leulmi Pichot, Sabrina Bentouati, Saif S. Ahmad, Marios Sotiropoulos, Raj Jenab and Russell Cowburn
RSC Adv., 2020, 10, 8161-8171
DOI: 10.1039/D0RA00164C, Paper

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We are very pleased to introduce Dr Sheta M. Sheta, corresponding author of the paper ‘A novel cerium(iii)–isatin Schiff base complex: spectrofluorometric and DFT studies and application as a kidney biomarker for ultrasensitive detection of human creatinine‘. The article has been very well received and handpicked by our reviewers and handling editors as one of our February HOT articles. Sheta was kind enough to tell us more about the work that went into this article and what he hopes to achieve in the future. You can find out more about the author and his article below and find more HOT articles in our online collection.

Meet the Author

Dr Sheta obtained his BSc and Masters degrees in applied chemistry from Helwan University, Egypt in 2004 and 2010 respectively. Following this, he joined the National Research Centre, Egypt in 2013 and obtained his PhD in inorganic-analytical chemistry from Ain Shams University, Egypt in 2015. His research interests include the preparation, characterization and application of metal-organic frameworks (MOFs), complexes, nanomaterials in general and specifically sensors, biosensors, medical laboratories, innovation and development of analytical techniques and methodologies for tumor markers, drugs and hormones determinations and biological activities studies as well as nano-catalysis and nano-ceramic materials. Dr. Sheta is also interested in nanostructured mesoporous materials and transition metal nanowires and particles, treatment of wastewater with conventional and advanced technologies, advanced oxidation technologies, nano-ceramic materials for advanced applications such as surface coatings and the reinforcement and conversion of CO₂ to renewable fuel. In addition, he has experience in waste treatment/recycling and environmental pollution control, removal of bacteria and algae from fresh and marine waters. He has organized and coordinated local and international events and often speaks at international conferences/symposia. He serves as an Editorial board member and reviewer of several reputed international journals.

Could you briefly explain the focus of your article to the non-specialist (in one or two sentences only) and why it is of current interest?
This work introduced a novel and promising analytical tool for the detection and quantification of creatinine as one of the most important kidney biomarkers.

How big an impact could your results potentially have?
The results showed a simple, low cost analytical tool with a fast response time, high sensitivity, high accuracy/precision, reproducibility, applicability, a lower limit of detection/quantification than in previously published reports and good recovery in the determination of creatinine in various real samples (serum/plasma).

Could you explain the motivation behind this study?
• Strengthening the national income related to import many tools and devices like pregnancy strips, diabetes devices and more.
• Applying nanotechnology to synthesize smart and advanced materials that can improve the efficiency of the target device.
• Trying to produce a prototype that is more selective, sensitive, costs less and is simple and fast compared to those available on the market.

In your opinion, what are the key design considerations for your study?
• Preparation of a novel nanomaterial step
• Characterization step
• DFT study
• Application step

Which part of the work towards this paper proved to be most challenging?
The characterization of the prepared compounds.

What aspect of your work are you most excited about at the moment?
Studying the anticancer and biological activities of the prepared compounds and the transferring of the research methodology to small device.

What is the next step? What work is planned?
Based on the point-of-care testing (POCT) systems and near patient testing devices, we will to try to transfer the research methodology to small device.

A novel cerium(iii)–isatin Schiff base complex: spectrofluorometric and DFT studies and application as a kidney biomarker for ultrasensitive detection of human creatinine
Sheta M. Sheta, Magda A. Akl, Heba E. Saad and El-Sayed R. H. El-Gharkawy
RSC Adv., 2020, 10, 5853-5863
DOI: 10.1039/C9RA10133K , Paper

Submit to RSC Advances today! Check out our author guidelines for information on our article types or find out more about the advantages of publishing in a Royal Society of Chemistry journal.

Keep up to date with our latest HOT articles, Reviews, Collections & more by following us on Twitter. You can also keep informed by signing up to our E-Alerts.