Scientists in Japan have designed a microdevice to monitor the effect that chemicals have on the growth of pollen tubes.
In higher plants, the male and female gametes are located in separate tissues within the plant. The male gamete is produced inside a pollen grain and for pollination to occur it must be carried to the female part of the plant by a growing pollen tube. Chemical signals from the female part of the plant attract the pollen tube towards it and only compatible pollen tubes make it all the way.
While these events are happening around us every day, they happen on the microscale, making them very difficult to study. Previous research into plant reproduction has suggested that the direction of pollen tube growth is controlled by a gradient of attractant molecules. However, most assays spot chemoattractant onto a medium then allow it to spread, which makes it difficult to determine the exact concentration of attractant molecule at the head of the pollen tube.
Now, Noritada Kaji and colleagues at Nagoya University have created a microfluidic assay that can more accurately investigate pollen tube growth. ‘Lab-on-a-chip technologies have found a new partner in plant physiology research,’ says Kaji. ‘Our simple microfluidic platform provides precisely defined concentration gradients of chemoattractants over an extended time period to enable the digital discrimination of chemoattractant effects on directional pollen tube growth.’
Read the rest of the story in Chemistry World!
Read the original research paper in RSC Advances:
