Super-resolution single-molecule imaging

Single-molecule fluorescence imaging for cellular applications

This exciting article just published in Chemical Science by Professor W.E. Moerner and colleagues describes a novel method of introducing fluorophores of single-molecule quality into live bacterial cells for super-resolution imaging studies.

Single-molecule fluorescence imaging works by converting a dark fluorogen into a bright emitter. The really interesting aspect of this current study is that this conversion is achieved enzymatically. They have synthesised a nitro-aryl fluorogen (dark fluorogen) which is converted by a nitroreductase enzyme into a push-pull red-emitting fluorophore (bright emitter).

This new method allows the neutral dye molecule to enter the cell in a fluorescently ‘deactivated’ state. The substrate is then photoactivated by reaction with the enzyme, producing the fluorescent products – which is bright and detectable on the single-molecule level. The concentration can also be controlled by the level of substrate uptake – providing the opportunity for a variety of novel labeling systems.

The authors also present a detailed characterization of the spectral and photophysical properties of the fluorescent product, as well as the enzymatic kinetics in vitro.

Read the full article:
Enzymatic Activation of Nitro-Aryl Fluorogens in Live Bacterial Cells for Enzymatic Turnover-Activated Localization Microscopy
Marissa K. Lee, Jarrod Williams, Robert Twieg, Jianghong Rao and W.E. Moerner
Chem. Sci., 2012, DOI: 10.1039/C2SC21074F

Take a look at the high-quality physical chemistry research recently published in Chemical Science:

Vibrationally resolved dynamics of the reaction of Cl atoms with 2,3-dimethylbut-2-ene in chlorinated solvents
Fawzi Abou-Chahine, Stuart Greaves, Greg Dunning, Andrew Orr-Ewing, Gregory M Greetham, Ian P Clark and Michael Towrie
Chem. Sci., 2012, DOI: 10.1039/C2SC21267F

Nanomechanical properties of molecular-scale bridges as visualised by intramolecular electronic energy transfer
Anthony Harriman, Effat Bahaidarah, Raymond Ziessel, Mohammed Alamiry and Delphine Hablot
Chem. Sci., 2012, DOI: 10.1039/C2SC21505E

Role of conformational structures and torsional anharmonicity in controlling chemical reaction rates and relative yields: butanal + HO2 reactions
Jingjing Zheng, Prasenjit Seal and Donald G. Truhlar
Chem. Sci., 2013, DOI: 10.1039/C2SC21090H

Tridentate Cobalt complexes as alternate redox couples for high efficiency Dye Sensitized Solar Cells
Ben Aribia Kais, Thomas Moehl, Shaik M Zakeeruddin and Michael Gratzel
Chem. Sci., 2012, DOI: 10.1039/C2SC21401F

Cu2O|NiOx nanocomposite as an inexpensive photocathode in photoelectrochemical water splitting
Chia-Yu Lin, Yi-Hsuan Lai, Dirk Mersch and Erwin Reisner
Chem. Sci., 2012, DOI: 10.1039/C2SC20874A

Perspective
Impurities in graphenes and carbon nanotubes and their influence on the redox properties
Martin Pumera, Adriano Ambrosi and Elaine Lay Khim Chng
Chem. Sci., 2012, DOI: 10.1039/C2SC21374E

Sign-up to our free contents e-alert or newsletter to receive the latest physical chemistry articles from Chemical Science.

Digg This
Reddit This
Stumble Now!
Share on Facebook
Bookmark this on Delicious
Share on LinkedIn
Bookmark this on Technorati
Post on Twitter
Google Buzz (aka. Google Reader)

Leave a Reply