The development of fast and efficient methods to separate proteins of interest from a biological source remains a challenging task in the proteomic era. With the use of magnetic nanoparticles, the separation of proteins can be significantly simplified by applying an external magnetic field.
Nanfeng Zheng and co-workers have prepared superparamagnetic core-shell Fe3O4@SiO2@poly(styrene-alt-maleic acid)/Ni-NTA particles having abundant Ni-NTA binding sites for the efficient separation of His-tagged proteins. The shell composition of poly(styrene-alt-maleic anhydride) allows the incorporation of more Ni-NTA affinity sites onto the surface of the magnetic spheres making them particularly promising for the magnetic separation of low-concentration His-tagged proteins.
Interested to know more? Read the full article here:
Superparamagnetic core-shell polymer particles for efficient purification of his-tagged proteins
Weijun Fang, Xiaolan Chen and Nanfeng Zheng
J. Mater. Chem., 2010, Advance Article
DOI:10.1039/C0JM02081H. Paper
