Microorganisms have a remarkable ability to adhere to virtually any type of abiotic surface, proliferate and subsequently form biofilms. Since materials may need to interact differently with microorganisms, the adherent or repellent properties of materials towards bacteria are an extremely important consideration in their design. For example, materials used as implants need to remain free from contamination, in order to reduce device-associated infections, and hence, antifouling surfaces are prepared using water repellent polymers or by anchoring antimicrobial compounds. Conversely, surfaces capable of controlled immobilization and removal of microorganisms have been equally explored for a rather broad range of applications, including sophisticated systems such as biosensors or biomolecular motors. Thus far, precise immobilization of microorganisms, and in particular bacteria, onto surfaces has been achieved by using a range of different fabrication approaches. However, there are relatively few examples of controlled immobilization of single bacteria that don’t involve expensive approaches or time-consuming multistep procedures.
In this paper, Palacios-Cuesta and colleagues describe the development of different surface patterns using a photolithographic-based technique that does not require the use of high resolution masks or clean rooms and produces surface patterns with micrometer and submicrometer resolution. The procedure is based on the crosslinking and degradation processes occurring in polystyrene upon exposure to UV light. Together, these processes produce different patterns depending, not only on the mask, but also on the experimental conditions employed. With this approach it is possible to produce patterns with nanoscale resolution without expensive fine focalisation settings. Of particular interest, the authors demonstrate the feasibility of this strategy to incorporate functional groups to modulate the affinity between the bacteria and the surface. In particular, hydrophilic segments, i.e. poly(acrylic acid) that favour bacterial immobilisation are introduced. The strategy employed allows not only the incorporation of functional groups, but also enables the fine tuning of the amount of hydrophilic functional groups. This unique feature is used by the authors to determine the role of surface hydrophilicity on the adhesion of Staphylococcus aureus onto the different surface patterns. Finally, those surfaces on which both photodegradation and photocrosslinking occurr produce thin patterns largely below the micrometer that are then used to prepare arrays of isolated S. aureus bacteria. The formation of bacterial arrays of S. aureus on the single-cell level has been a challenge since they exhibit a large tendency to grow in clusters. This technology is exciting given its potential for enabling the isolation of single bacteria for diagnosis, and the study of bacterial populations at the single cell level.
Patterning of individual Staphylococcus aureus bacteria onto photogenerated polymeric surface structures by Marta Palacios-Cuesta, Aitziber L. Cortajarena, Olga García and Juan Rodríguez-Hernández, Polym. Chem., 2015, 6, 2677-2684.
Remzi Becer is a web-writer and Advisory Board member for Polymer Chemistry. He is currently a Senior Lecturer in Materials Science and the director of the Polymer Science and Nanotechnology masters programme at Queen Mary, University of London. Visit www.becergroup.com for more information.