What’s HOT in Issue 9?

Rapid and sensitive chromatograpHOT article: Rapid and sensitive chromatographic determination of free sialic acid in complex bio-pharma sampleshic determination of free sialic acid in complex bio-pharma samples

Alwael et al., Anal. Methods, 2012,4, 2668-2673

There are plenty of HOT articles to take a look at in our latest issue, including one from our Editor-in-Chief, Brett Paull. His paper, with co-authors Hassan Alwael and Damian Connolly, describes a rapid, sensitive and reproducible liquid chromatographic method specifically for the quantitative determination of total free sialic acid, employing thiobarbituric acid as pre-column tagging agent, following the oxidation of sialic acid with periodic acid.

Rapid and sensitive chromatographic determination of free sialic acid in complex bio-pharma fermentation media samples
Hassan Alwael, Damian Connolly and Brett Paull
Anal. Methods, 2012,4, 2668-2673
DOI: 10.1039/C2AY25299F

Take a look at the rest of this issue’s HOT articles below – they will all be free to access until 7 September.

Read more »

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Issue 9 now online

Analytical Methods, 2012, Vol. 4, Issue 9, front cover

Front cover: Männel-Croisé and Zelder, Anal. Methods, 2012, 4, 2632-2634

Take a look at the latest issue of Analytical Methods, now available online.

On the front cover is an article that has already been highlighted in Chemistry World. Christine Männel-Croisé and Felix Zelder of the University of Zürich have developed a method that enables them to quickly detect blood cyanide levels through a simple colour change. The technique only takes two minutes and does not require any laboratory equipment.

Communication: Rapid visual detection of blood cyanide
Christine Männel-Croisé and Felix Zelder
Anal. Methods, 2012,4, 2632-2634
DOI: 10.1039/C2AY25595B

The inside front cover also features work on a method involving a visible colour changes. Takeo Yamaguchi of Tokyo Institute of Technology and co-workers show conversion of a biomolecule signal into a visual color by nanoparticle permeation through a biomolecule-recognition gating membrane.

Communication: Conversion of a molecular signal into a visual color based on the permeation of nanoparticles through a biomolecule-recognition gating membrane
Yuuki Sugawara, Hidenori Kuroki, Takanori Tamaki, Hidenori Ohashi, Taichi Ito and Takeo Yamaguchi
Anal. Methods, 2012,4, 2635-2637
DOI: 10.1039/C2AY25265A

Analytical Methods, 2012, Vol. 4, Issue 9, inside front cover

Inside front cover: Sugawara et al., Anal. Methods, 2012,4, 2635-2637

Both these covers are free to access for 6 weeks, so do let your colleagues know.

This issue also features the latest Analytical Methods Committee Technical Brief, also free to access:

Dark uncertainty
Analytical Methods Committee, AMCTB No 53
Anal. Methods, 2012, 4, 2609-2612
DOI: 10.1039/C2AY90034C

Follow Analytical Methods on Twitter: @methodsrsc

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Latest Track on the Detection Playlist: Diagnosis of Influenza using DVD Reader Technology

Maquieira et al., Anal. Methods, 2012, Advance Article

By Pui Sai Lau, Web Writer

Many people loathe the aches and pains attributed to influenza (or flu) infection, which is especially prevalent during the winter months. However, depending on the aggressiveness of the pathogen subtype, more severe health complications including mortality can occur, particularly in infants and the elderly. While several detection methods are available for influenza diagnosis, limitations still exist such as long-analysis time, requirement of intensive labor and high cost. To tackle some of these issues, Ángel Maquieira and colleagues from Valencia, Spain have developed a sandwich immunoassay on commercial DVDs that makes use of a standard DVD reader for quantification. Check out their discovery by accessing the link below!

Influenza A virus infection diagnosis based on DVD reader technology
María-José Bañuls, María-Victoria González-Pedro, Rosa Puchades and Ángel Maquieira
Anal. Methods, 2012, Advance Article
DOI: 10.1039/C2AY25493J

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HOT articles in Analytical Methods

Take a look below at some wonderful HOT articles we’ve recently published in Analytical Methods. There are papers covering a large variety of fields, including stationary phases for HPLC, water analysis, glucose sensing and profiling fats and meats. These papers will be free to read for two weeks.

A protocol for the validation of qualitative methods of detection
Roy Macarthur and Christoph von Holst
Anal. Methods, 2012, Advance Article
DOI: 10.1039/C2AY05719K

Porous silica microspheres obtained by grinding monolithic columns as stationary phase for high performance liquid chromatography
Qishu Qu,  Qian Gu,  Lihua Shi,  Zuli Gu and Xiaoya Hu
Anal. Methods, 2012, Advance Article
DOI: 10.1039/C2AY25330E

Development of solution, film and membrane based fluorescent sensor for the detection of fluoride anions from water
Gunin Saikia,  Atul K. Dwivedi and Parameswar K. Iyer
Anal. Methods, 2012, Advance Article
DOI: 10.1039/C2AY25565K

Mesoporous CuO: Alternative enzyme-free glucose sensing structure with excellent kinetics of electrode process
Mei-Fang Wang,  Qin-An Huang,  Xiang-Zi Li and Yan Wei
Anal. Methods, 2012, Advance Article
DOI: 10.1039/C2AY25501D

Study on the electrochemical properties of maltol at a carbon paste electrode and its analytical application
Jing Zhou,  Kai Zhang,  Yamin Li,  Kunjing Li and Baoxian Ye
Anal. Methods, 2012, Advance Article
DOI: 10.1039/C2AY25514F

Intact triacylglycerol profiles of fats and meats via thermal imprinting easy ambient sonic-spray ionization mass spectrometry
Andréia M. Porcari,  Nicolas V. Schwab,  Rosana M. Alberici,  Elaine C. Cabral,  Damila R. de Moraes,  Paula F. Montanher,  Christina R. Ferreira,  Marcos N. Eberlin and Jesuí V. Visentainer
Anal. Methods, 2012, Advance Article
DOI: 10.1039/C2AY25550B


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Advances in Clinical Analysis 2012 Meeting

The Chromatographic Society and the Separation Science Group of the Analytical Division of the Royal Society of Chemistry are pleased to announce their next meeting: Advances in Clinical Analysis 2012

30th October 2012
Robens Suite,
29th Floor,
Guy’s Hospital Tower,
St Thomas’ Street, London, SE1 9RT, UK

Clinical analysis is a wide ranging and complex subject area, but as we move to more complex and difficult analyses, much is now being asked of the techniques in terms of the specificity, sensitivity, simplicity and productivity.

The meeting sets out to look at separation and complementary/alternative techniques along with case studies from the hospital and academic areas. The program has been designed to give attendee’s an update for both routine and problem solving in clinical analysis.

For more information please contact Adrian Clarke.
Tel +41 616963955 e-mail:adrian.clarke@novartis.com

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HOT Article: Determining free sialic acid levels in bio-pharma samples

Paull et al., Anal. Methods, 2012, Advance Article

This month we have a HOT article from Brett Paull, the Editor in Chief of Analytical Methods. Sialic acids have many biological roles. The importance of sialic acids to a glycoprotein’s half-life means that there is a strong need to determine the sialic acid content of a glycoprotein when examining its function and activity as a potential therapeutic. A team from Ireland and Australia, have developed a very rapid and analytically robust method that can quantitatively determine total free sialic acid, based upon monolithic reversed-phase chromatography. This method has been developed and specifically applied to process a range of very complex samples from the biopharmaceutical industry. This article will be free to read for 2 weeks.

Rapid and sensitive chromatographic determination of free sialic acid in complex bio-pharma fermentation media samples
Hassan Alwael, Damian Connolly and Brett Paull
Anal. Methods, 2012, Advance Article
DOI: 10.1039/C2AY25299F

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Top ten most accessed articles in June

This month sees the following articles in Analytical Methods that are in the top ten most accessed:-

Precision in chemical analysis: a critical survey of uses and abuses 
Michael Thompson  
Anal. Methods, 2012,4, 1598-1611 DOI: 10.1039/C2AY25083G    

Fluorescent boronic acid-modified polymer nanoparticles for enantioselective monosaccharide detection 
Wan-Kyu Oh ,  Yoon Seon Jeong ,  Kyung Jin Lee and Jyongsik Jang  
Anal. Methods, 2012,4, 913-918 DOI: 10.1039/C2AY05800F     

Novel Electrode Reactions of Diazepam, Flunitrazepam and Lorazepam and Their Exploitation in a New Redox Mode LC-DED Assay for Serum 
Kevin C. Honeychurch ,  Ai Teng Chong ,  Khalil Elamin and John P. Hart  
Anal. Methods, 2012,4, 132-140 DOI: 10.1039/C1AY05419H     

Graphene/Au nanoparticles nanocomposite film for selective electrochemical determination of dopamine 
Juan Li ,  Juan Yang ,  Zhanjun Yang ,  Yongfang Li ,  Suhua Yu ,  Qin Xu and Xiaoya Hu  
Anal. Methods, 2012,4, 1725-1728 DOI: 10.1039/C2AY05926F     

Nonenzymatic chemiluminescence resonance energy transfer: an efficient technique for selective and sensitive detection of silver ion 
Shulin Zhao ,  Guoxin Qin ,  Yong Huang ,  Shuting Li ,  Xin Lu ,  Jing Jiang and Fanggui Ye  
Anal. Methods, 2012,4, 1927-1931 DOI: 10.1039/C2AY25404B     

Graphene oxide as a nano-platform for ATP detection based on aptamer chemistry 
Wen Dan Pu ,  Li Zhang and Cheng Zhi Huang  
Anal. Methods, 2012,4, 1662-1666 DOI: 10.1039/C2AY25166C     

Sensor and biosensor to detect vascular graft infection: diagnosis and challenges 
Mamun Jamal ,  Faisal M. Shaikh ,  Bilal Aslam and Kafil M. Razeeb  
Anal. Methods, 2012,4, 1865-1875 DOI: 10.1039/C2AY25027F     

Al3+ induced green luminescent fluorescent probe for cell imaging and naked eye detection 
Debasis Karak ,  Sisir Lohar ,  Animesh Sahana ,  Subarna Guha ,  Arnab Banerjee and Debasis Das  
Anal. Methods, 2012,4, 1906-1908 DOI: 10.1039/C2AY25226K     

Polyaniline/ Polyacrylic Acid/ Multi-walled Carbon Nanotube Modified Electrodes for sensing Ascorbic Acid 
Ida Tiwari ,  Karan Pratap Singh ,  Manorama Singh and Craig E. Banks  
Anal. Methods, 2012,4, 118-124 DOI: 10.1039/C1AY05415E     

Let there be chip – towards rapid prototyping of microfluidic devices: one-step manufacturing processes 
Ansgar Waldbaur ,  Holger Rapp ,  Kerstin Länge and Bastian E. Rapp 
Anal. Methods, 2011,3, 2681-2716 DOI: 10.1039/C1AY05253E     

Why not take a look at the articles today and blog your thoughts and comments below.

Fancy submitting an article to Analytical Methods? Then why not submit to us today or alternatively email us  your suggestions.

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HOT Article: Colorimetric detection of lysine using gold nanoparticles

Xu et al, Anal. Methods, 2012, Advance Article

By Laura Ruvuna, Web Writer

Sensors don’t always require expensive equipment or complex attachment chemistries, and when possible a visual color change is enough. In this HOT article, Maotian Xu and colleagues from Shangqiu Normal University, China, have detected lysine effectively and cheaply using the colorimetric properties of gold nanoparticles during aggregation. The lysine content in food is important for growth and development, and using this assay it was detected in three different commercial food sources. In addition to the visual change, the UV-Vis spectra scaled linearly with lysine concentration. See more about this simple but sensitivity system below, it will be free to read for 2 weeks.

Colorimetric detection of lysine using gold nanoparticles aggregation
Yanli Zhou,  Zhichong Yang and Maotian Xu
Anal. Methods, 2012, Advance Article
DOI: 10.1039/C2AY25475A

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HOT articles in Analytical Methods

FitzGerald et al., Anal. Methods, 2012, Advance ArticleTake a look at some of the HOT articles we’ve published recently in Analytical Methods! These papers cover a wide variety of topics such as analysis of food, water pollution, zeolite columns and quantum dots. These papers will be free to access for 2 weeks.

Sensitive and rapid monitoring of water pollution level based on the signal enhancement of an activated glassy carbon electrode
Can Wu, Shijin Yu, Bin Lin, Qin Cheng and Kangbing Wu
Anal. Methods, 2012, Advance Article
DOI: 10.1039/C2AY25523E

Determination of trace leaching phthalate esters in water by magnetic solid phase extraction based on magnetic multi-walled carbon nanotubes followed by GC-MS/MS
Yanna Jiao, Shanliang Fu, Li Ding, Qiang Gong, Shaohua Zhu, Libing Wang and Hui Li
Anal. Methods, 2012, Advance Article
DOI: 10.1039/C2AY25386K

Mollica & Charles et al, Anal. Methods, 2012, Advance Article

Towards the rationalization of the MALDI process: a combined mass spectrometry/solid-state NMR approach
Yannis Major, Hélène Pizzala, Fabio Ziarelli, Trang N. T. Phan, Giulia Mollica and Laurence Charles
Anal. Methods, 2012, Advance Article
DOI: 10.1039/C2AY25708D

Preparative size-exclusion chromatography for separation and purification of water-stable Cd-based quantum dots
Luis Alamo-Nole, Sonia Bailon-Ruiz, Oscar Perales-Perez and Felix R. Roman
Anal. Methods, 2012, Advance Article
DOI: 10.1039/C2AY25629K

Hydrochromic fluorescence of organo-boronium-avobenzone complexes
Xuepeng Zhang and Guoqing Zhang
Anal. Methods, 2012, Advance Article
DOI: 10.1039/C2AY25424G

Multi-branch chemiluminescence–molecular imprinting sensor for sequential determination of carbofuran and omethoate in foodstuff
Shenguang Ge, Peini Zhao, Mei Yan, Dejin Zang and Jinghua Yu
Anal. Methods, 2012, Advance Article
DOI: 10.1039/C2AY25410G

Zhang & Zhang, Anal. Methods, 2012, Advance Article

Surface chemistry and linker effects on lectin–carbohydrate recognition for glycan microarrays
Michelle Kilcoyne, Jared Q. Gerlach, Marian Kane and Lokesh Joshi
Anal. Methods, 2012, Advance Article
DOI: 10.1039/C2AY25532D

Determination of anionic impurities in hexafluorosilicic acid by capillary zone electrophoresis
Nerea Ayarza, Juan M. G. Góngora, Rosa María Alonso and Rosa María Jiménez
Anal. Methods, 2012, Advance Article
DOI: 10.1039/C2AY25553G

Hierarchical Fe-ZSM-5 zeolite monolithic column for enhanced phosphorylated protein immobilization and identification
Wenzhong Zhang, Deju Wang, Huai Sun, Jun Yao, Fang Xu and Pengyuan Yang
Anal. Methods, 2012, Advance Article
DOI: 10.1039/C2AY25454A

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Eye spy cyanide

The colour of cyanide poisoning is purple, according to researchers in Switzerland who have developed a method that enables them to quickly detect blood cyanide levels through a simple colour change.

The two-step method to detect cyanide. (A) Adding a chemosensor to a blood sample, followed by extracting the purple chemosensor–cyano complex from the sample. (B) Washing the column with water

The two-step method to detect cyanide. (A) Adding a chemosensor to a blood sample, followed by extracting the purple chemosensor–cyano complex from the sample. (B) Washing the column with water

Cyanide poisoning as a result of smoke inhalation can have serious or fatal consequences unless an antidote is rapidly administered. Current methods for determining cyanide poisoning, including microdiffusion, microdistillation and liquid chromatography-mass spectrometry detection, can take up to an hour to give results and are not suitable for point-of-care settings.

Read the full article in Chemistry World online.

Rapid visual detection of blood cyanide
Christine Männel-Croisé and Felix Zelder
Anal. Methods, 2012, Advance Article
DOI: 10.1039/C2AY25595B

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