Lab on a Chip Blog

These HOT articles were recommended by our referees and are free to access for 4 weeks*

Antimicrobial susceptibility assays in paper-based portable culture devices
Frédérique Deiss, Maribel E. Funes-Huacca, Jasmin Bal, Katrina F. Tjhung and Ratmir Derda  
Lab Chip, 2014,14, 167-171
DOI: 10.1039/C3LC50887K, Communication

Cell force measurements in 3D microfabricated environments based on compliant cantilevers
Mattia Marelli, Neha Gadhari, Giovanni Boero, Matthias Chiquet and Jürgen Brugger  
Lab Chip, 2014,14, 286-293
DOI: 10.1039/C3LC51021B, Paper

A differential dielectric affinity glucose sensor
Xian Huang, Charles Leduc, Yann Ravussin, Siqi Li, Erin Davis, Bing Song, Dachao Li, Kexin Xu, Domenico Accili, Qian Wang, Rudolph Leibel and Qiao Lin  
Lab Chip, 2014,14, 294-301
DOI: 10.1039/C3LC51026C, Paper

Microfluidic transwell inserts for generation of tissue culture-friendly gradients in well plates
Christopher G. Sip, Nirveek Bhattacharjee and Albert Folch  
Lab Chip, 2014,14, 302-314
DOI: 10.1039/C3LC51052B, Paper
From themed collection Lab on a Chip Top 10%

Gradient static-strain stimulation in a microfluidic chip for 3D cellular alignment
Hsin-Yi Hsieh, Gulden Camci-Unal, Tsu-Wei Huang, Ronglih Liao, Tsung-Ju Chen, Arghya Paul, Fan-Gang Tseng and Ali Khademhosseini  
Lab Chip, 2014,14, 482-493
DOI: 10.1039/C3LC50884F, Paper

Biosensor design based on Marangoni flow in an evaporating drop
Joshua R. Trantum, Mark L. Baglia, Zachary E. Eagleton, Raymond L. Mernaugh and Frederick R. Haselton  
Lab Chip, 2014,14, 315-324
DOI: 10.1039/C3LC50991E, Paper
From themed collection Lab on a Chip Top 10%

Flow of suspensions of carbon nanotubes carrying phase change materials through microchannels and heat transfer enhancement
Sumit Sinha-Ray, Suman Sinha-Ray, Hari Sriram and Alexander L. Yarin  
Lab Chip, 2014,14, 494-508
DOI: 10.1039/C3LC50949D, Paper

Micro-scaffold array chip for upgrading cell-based high-throughput drug testing to 3D using benchtop equipment
Xiaokang Li, Xinyong Zhang, Shan Zhao, Jingyu Wang, Gang Liu and Yanan Du  
Lab Chip, 2014,14, 471-481
DOI: 10.1039/C3LC51103K, Paper

Synchronized reinjection and coalescence of droplets in microfluidics
Manhee Lee, Jesse W. Collins, Donald M. Aubrecht, Ralph A. Sperling, Laura Solomon, Jong-Wook Ha, Gi-Ra Yi, David A. Weitz and Vinothan N. Manoharan  
Lab Chip, 2014,14, 509-513
DOI: 10.1039/C3LC51214B, Paper

A microfluidic reciprocating intracochlear drug delivery system with reservoir and active dose control
Ernest S. Kim, Erich Gustenhoven, Mark J. Mescher, Erin E. Leary Pararas, Kim A. Smith, Abigail J. Spencer, Vishal Tandon, Jeffrey T. Borenstein and Jason Fiering  
Lab Chip, 2014, Advance Article
DOI: 10.1039/C3LC51105G, Paper
From themed collection Lab on a Chip Top 10%

Impedance matched channel walls in acoustofluidic systems
Ivo Leibacher, Sebastian Schatzer and Jürg Dual  
Lab Chip, 2014,14, 463-470
DOI: 10.1039/C3LC51109J, Paper

Magnetoactive sponges for dynamic control of microfluidic flow patterns in microphysiological systems
Sungmin Hong, Youngmee Jung, Ringo Yen, Hon Fai Chan, Kam W. Leong, George A. Truskey and Xuanhe Zhao  
Lab Chip, 2014,14, 514-521
DOI: 10.1039/C3LC51076J, Paper
From themed collection Lab on a Chip Top 10%

The microfluidic post-array device: high throughput production of single emulsion drops
E. Amstad, S. S. Datta and D. A. Weitz  
Lab Chip, 2014, Advance Article
DOI: 10.1039/C3LC51213D, Paper
From themed collection Lab on a Chip Top 10%

Interdroplet bilayer arrays in millifluidic droplet traps from 3D-printed moulds
Philip H. King, Gareth Jones, Hywel Morgan, Maurits R. R. de Planque and Klaus-Peter Zauner  
Lab Chip, 2014, Advance Article
DOI: 10.1039/C3LC51072G, Paper

A 1024-sample serum analyzer chip for cancer diagnostics
Jose L. Garcia-Cordero and Sebastian J. Maerkl  
Lab Chip, 2014, Advance Article
DOI: 10.1039/C3LC51153G, Paper
From themed collection Lab on a Chip Top 10%

Utilization and control of bioactuators across multiple length scales
Vincent Chan, H. Harry Asada and Rashid Bashir  
Lab Chip, 2014, Advance Article
DOI: 10.1039/C3LC50989C, Critical Review

Microfabricated perfusable cardiac biowire: a platform that mimics native cardiac bundle
Yun Xiao, Boyang Zhang, Haijiao Liu, Jason W. Miklas, Mark Gagliardi, Aric Pahnke, Nimalan Thavandiran, Yu Sun, Craig Simmons, Gordon Keller and Milica Radisic  
Lab Chip, 2014, Advance Article
DOI: 10.1039/C3LC51123E, Paper
From themed collection Lab on a Chip Top 10%

Radiolabelling diverse positron emission tomography (PET) tracers using a single digital microfluidic reactor chip
Supin Chen, Muhammad Rashed Javed, Hee-Kwon Kim, Jack Lei, Mark Lazari, Gaurav J. Shah, R. Michael van Dam, Pei-Yuin Keng and Chang-Jin “CJ” Kim  
Lab Chip, 2014, Advance Article
DOI: 10.1039/C3LC51195B, Paper

Non-destructive handling of individual chromatin fibers isolated from single cells in a microfluidic device utilizing an optically driven microtool
Hidehiro Oana, Kaori Nishikawa, Hirotada Matsuhara, Ayumu Yamamoto, Takaharu G. Yamamoto, Tokuko Haraguchi, Yasushi Hiraoka and Masao Washizu  
Lab Chip, 2014, Advance Article
DOI: 10.1039/C3LC51111A, Paper

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Researchers from the University of Tokyo and the CEA-Leti research institute in France have designed a platform that allows channels to be written and rewritten at will in a fluid layer atop a grid of electrodes.

In their recent cover article in Lab on a Chip, Raphael Renaudot and colleagues describe this novel device, which is based on the Electrowetting on Dielectric (EWOD) and Liquid Dielectrophoresis (LDEP) phenomena¹. Their goal was to create a flexible, reusable platform that would enable the creation of microfluidic devices without the use of expensive microfabrication techniques. The fluid layer of the device is filled with liquid paraffin, which can easily be solidified and re-melted via thermoelectric cooling and heating (its melting temperature is 35° C.) Water is injected into the paraffin layer, and its flow path is controlled via the underlying electrode layer, which is made up of a grid of 83 electrodes (the small squares seen in the figure). By tuning the interfacial tension between the water and the surface of each electrode via the independently controlled electrode voltages, it is possible to guide a channel (or “finger”) of water along the desired path on the grid (see figure).

After the water is guided into the desired path, the device is cooled, solidifying the paraffin and setting the channels in place. Later, the device can be reheated to erase the existing channels, and a new design can be drawn. The chip can be reused multiple times, reducing waste and making it very useful for prototype testing and low-cost applications. The authors demonstrated two chip designs using the reconfigurable platform: a droplet generator and a device for E. coli confinement within a fluidic cavity.

A water channel is drawn through a paraffin matrix via control of an underlying grid of electrodes (from Figure 2b)

Read this HOT article in Lab on a Chip today!

A Programmable and Reconfigurable Microfluidic Chip, Raphael Renaudot, Vincent Agache, Yves Fouillet, Guillaume Laffite, Emilie Bisceglia, Laurent Jalabert, Momoko Kumemura, Dominique Collard and Hiroyuki Fujita. DOI: 10.1039/c3lc50850a

References:

1. T. B. Jones and K. L. Wang, Langmuir 2004, 20 (7), 2813–2818.

In a recent paper in Lab on a Chip, which featured as the Cover Article of Issue 22, Vol 13, researchers from the Ecole Polytechnique, Palaiseau, France describe a micro-droplet based method for measuring reaction kinetics using tiny (20 nL) sample volumes.

When working with precious reagents such as enzymes, minimizing sample volume is of the utmost importance.  Droplet-based microfluidic methods can greatly reduce sample volumes, but still waste considerable amounts of sample in fluid handling processes (e.g. pushing the sample through a channel).  A team led by Marten Vos and Charles Baroud has utilized a unique “rails and anchors” method¹ of forming and controlling the movement of droplets to observe the kinetics of a chemical reaction using only 20 nL of each reagent solution.

In this scheme, the flow chamber geometry is carefully designed so that when an aqueous solution is injected into the oil-filled chamber, droplets of a reproducible size are released and constrained between the top and bottom surfaces of the chamber.  Then, widening grooves (“rails”) in the bottom of the chamber guide the droplets via surface tension to “anchors” in the center.  (See figure a and b.)  In this way, no additional solution is wasted pushing droplets around.

Droplet-based scheme for observing reaction kinetics (Fig 2 from paper)

The authors used the “rails and anchors” to place two droplets, each containing a different reagent, in proximity, and then used an IR laser pulse to break the surface tension between them (figure c). The interface between the two solutions (in this case, DCPIP and ascorbic acid) can clearly be seen in figure d below.  By tracking the front of the color change that moves across the droplet as the reaction proceeds (figure e), it is possible to calculate the reaction rate (accounting also for the diffusion rates of the reactants).

The researchers also demonstrated the potential of multiplexing their technique by monitoring six different reactions in parallel droplets. They were able to obtain results in good agreement with a commercial stopped-flow spectrometer with greatly reduced cost and time.

Read this HOT article in Lab on a Chip today!

Parallel Measurements of Reaction Kinetics using Ultralow Volumes, Etienne Fradet, Paul Abbyad, Marten H. Vos, and Charles N. Baroud, DOI: 0.1039/c3lc50768h

References:

1. P. Abbyad et al., Lab on a Chip 2011, 11, 813-821.

These HOT articles were recommended by our referees and are free to access for 4 weeks*

One-step microfluidic generation of pre-hatching embryo-like core–shell microcapsules for miniaturized 3D culture of pluripotent stem cells
Pranay Agarwal, Shuting Zhao, Peter Bielecki, Wei Rao, Jung Kyu Choi, Yi Zhao, Jianhua Yu, Wujie Zhang and Xiaoming He  
Lab Chip, 2013,13, 4525-4533
DOI: 10.1039/C3LC50678A

A microfluidic approach to synthesizing high-performance microfibers with tunable anhydrous proton conductivity
Mohammad Mahdi Hasani-Sadrabadi, Jules J. VanDersarl, Erfan Dashtimoghadam, Ghasem Bahlakeh, Fatemeh Sadat Majedi, Nassir Mokarram, Arnaud Bertsch, Karl I. Jacob and Philippe Renaud  
Lab Chip, 2013,13, 4549-4553
DOI: 10.1039/C3LC50862E

The microfluidic Kelvin water dropper
Álvaro G. Marín, Wim van Hoeve, Pablo García-Sánchez, Lingling Shui, Yanbo Xie, Marco A. Fontelos,  Jan C. T. Eijkel, Albert van den Berg and Detlef Lohs  
Lab Chip, 2013,13, 4503-4506
DOI: 10.1039/C3LC50832C

A cell rolling cytometer reveals the correlation between mesenchymal stem cell dynamic adhesion and differentiation state
Sungyoung Choi, Oren Levy, Mónica B. Coelho, Joaquim M. S. Cabral, Jeffrey M. Karp and Rohit Karnik  
Lab Chip, 2013, Advance Article
DOI: 10.1039/C3LC50923K

Ultrahigh-throughput sorting of microfluidic drops with flow cytometry
Shaun W. Lim and Adam R. Abate  
Lab Chip, 2013,13, 4563-4572
DOI: 10.1039/C3LC50736J

A programmable and reconfigurable microfluidic chip
Raphael Renaudot, Vincent Agache, Yves Fouillet, Guillaume Laffite, Emilie Bisceglia, Laurent Jalabert, Momoko Kumemura,   Dominique Collard and Hiroyuki Fujita  
Lab Chip, 2013,13, 4517-4524
DOI: 10.1039/C3LC50850A

The term ‘microfluidics’ immediately invokes the image of elegant, tiny devices with a small footprint in which fluids are manipulated with ease. It is an image that is most definitely correct. Still, every researcher in the field of microfluidics is aware of ‘the dirty little secret’ of these devices. Namely, that they often require bulky pumps and lots of tubing to be operated.

The requirement for macroscopic equipment to control microfluidic devices is a serious problem. It hinders the development of complex and integrated microfluidic circuits and it prevents the integration of the devices in small portable equipment.

Many organisms can direct their movements according to their chemical environment. In bacteria, this chemical-directed migration (i.e. chemotaxis) is enabled by receptor-mediated signaling to the bacterial flagellar motors.¹ Equipped with this remarkable sensory-motor system, bacteria can measure chemical gradients and swim towards nutritious substances (positive chemotaxis) or flee from cytotoxic chemicals (negative chemotaxis).

Bacterial chemotaxis is critical for various processes including biofilm formation, biomediation, and diseases pathogenesis.² For example, at the onset of various digestive diseases, bacteria rely on chemotaxis to find a suitable colonization site in the human gastrointestinal tract. Thus, there is great interest in studying the rates and molecular mechanisms in these chemotactic processes.

In the past 10 years, microfluidic gradient-generators have improved the way scientists study bacterial chemotaxis;³ however, these studies have not addressed gas gradients (e.g. O₂ and CO₂), which are known to be important in bacterial motility.⁴ Microfluidics devices have enabled the generation of well-controlled chemical gradients and the measurement of bacterial response at high spatiotemporal resolution. Gradients are typically formed by flowing a sample liquid (e.g. aspartate in buffer) and a reference liquid (e.g. buffer) into the device.

A team of researchers at the RIKEN Advanced Science Institute, Japan and Hanyang University, Korea carried out the world’s first microfluidic study of bacterial chemotaxis in gas gradients.⁵ The microfluidic device (see picture) comprises an isolated chamber (micro-aquarium) for bacterial culture and two bypass channels for sample and reference inputs. In this design, the sample molecules in the channel permeate through the PDMS walls (150 µm thick) and diffuse into the liquid of the micro-aquarium, facilitating the generation of a stable, shear-free gradient. The bacterial response can be monitored optically and quantified via image processing.

Using this device, the research team studied the chemotactic behaviour of Euglena gracilis microbial cells in the presence of CO₂ gradients. These studies revealed that the chemotaxis of Euglena cells is rapid and is dependent on CO₂ concentration. In particular, negative chemotaxis was observed at high concentrations (>15%) and positive chemotaxis was observed at low concentrations (<15%)— the maximum positive chemotaxis was observed at ~5% CO₂, corresponding to the most favourable condition for photosynthesis. Interestingly, the team observed evidence of chemotactic “adaption” when they consecutively switched the sample and reference inputs.

The microfluidic device is also compatible with small molecule liquid samples such as ethanol, H₂O₂, and culture media. By continuously perfusing culture medium in the bypass channels, the viability of the Euglena cells in the micro-aquarium can be maintained for more than 2 months.

In summary, the research team created a microfluidic gradient-generator that is versatile and straightforward to use. The device is useful for bacterial chemotaxis studies in chemical gradients formed from gas or liquid samples. Furthermore, the device may potentially be useful for other applications including drug screening, toxicity assays, and environmental monitoring.

1. H. C. Berg, Annual Review of Biochemistry, 2003, 72, 19-54.
2. T. Ahmed, T. S. Shimizu and R. Stocker, Integrative Biology, 2010, 2, 604-629.
3. J. Wu, X. Wu and F. Lin, Lab on a Chip, 2013, 13, 2484-2499.
4. C. Douarche, A. Buguin, H. Salman and A. Libchaber, Physical Review Letters, 2009, 102, 198101.
5. K. Ozasa, J. Lee, S. Song, M. Hara and M. Maeda, Gas/liquid sensing via chemotaxis of Euglena cells confined in an isolated micro-aquarium, Lab on a Chip, 2013, 13, 4033-4039