Archive for the ‘Hot Articles’ Category

LOC article on the capture of tumour cells featured in the press

A combined micromagnetic-microfluidic device for rapid capture and culture of rare circulating tumor cellsAn article recently published in Lab on a Chip on the capture and culture of rare circulating tumour cells from Donald Ingber and Joo Kang has featured on CNET and PhysOrg.  The device, which the team describes as a ‘micromagnetic-microfluidic device’ is capable of separating almost 90% of circulating tumour cells from blood by binding them to magnetic beads.  Due to the design of the channels in the device, the CTCs remain viable following isolation and can be used further for culture and testing.

Read the full article here:

A combined micromagnetic-microfluidic device for rapid capture and culture of rare circulating tumor cells
Joo H. Kang, Silva Krause, Heather Tobin, Akiko Mammoto, Mathumai Kanapathipillai and Donald E. Ingber
DOI: 10.1039/C2LC40072C

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LOC issue 10 available now

Issue 10 of Lab on a Chip is now available online. This week’s front cover features the winning image from the µTAS ‘Art in Science‘ award, which was presented to Dong Jin Shin at the 15th International Conference on Miniaturized Systems for Chemistry and the Life Sciences (µTAS) held in Seattle, Washington, USA, on October 2–6, 2011. The image is entitled ‘Ying and Yang in a Droplet’ – more information can be found in the editorial.

The art in science of microTAS
Michael Gaitan and Harp Minhas
DOI: 10.1039/C2LC90034C

The inside front cover features work from Ross Booth and Hanseup Kim at the University of Utah. They have developed a microfluidic blood-brain barrier that mimics the in vivo equivalent, using a thin culture membrane. They hope the model will give insight into blood-brain barrier studies and drug delivery methods.

Characterization of a microfluidic in vitro model of the blood-brain barrier (μBBB)
Ross Booth and Hanseup Kim
DOI: 10.1039/C2LC40094D

Another HOT article included in this issue comes from Frances Ligler and colleagues at the Naval Research Laboratory in Washington, DC, and introduces the “MagTrap” device that can perform automated target capture, efficient mixing with reagents, and controlled target release using 6 pairs of magnets in a rotating wheel.

Spinning magnetic trap for automated microfluidic assay systems
Jasenka Verbarg, Kian Kamgar-Parsi, Adam R. Shields, Peter B. Howell and Frances S. Ligler
DOI: 10.1039/C2LC21189K

Also included in the issue are this week’s Research highlight from Dr Ali Khademhosseini and Acoustofluidics 11 by Per Augustsson and Thomas Laurell, entitled ‘Affinity specific extraction and sample decomplexing using continuous flow acoustophoresis’.

Read the rest of issue 10 here

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A new HOT article on dielectrophoretic assisted cell sorting

This week’s HOT article comes from our Associate Editor, Professor Abraham Lee, along with Javier Prieto and their coworkers from the University of California. The paper introduces a device that uses dielectrophoretic principles for the characterisation and isolation of sub-populations of cells.

Using the automated dielectrophoretic assisted cell sorting (DACS) device, the group were able to enrich the neurons from a population of neural stem/progenitor cells and neurons using multiple discrete frequency bands, rather than at single frequencies.

The authors also used Monte-Carlo simulations to study the dielectric dispersions within the heterogeneous cell population, allowing them to predict the sorting efficiency of the device according to the trapping behaviour of different cell populations at different frequencies. This information was used to decide the optimal parameters with which to carry out the neuronal sorting, resulting in a 1.4-fold neuronal enrichment.

Frequency discretization in dielectrophoretic assisted cell sorting arrays to isolate neural cells
Javier L. Prieto,  Jente Lu,  Jamison L. Nourse,  Lisa A. Flanagan and Abraham P. Lee
DOI: 10.1039/C2LC21184J

All our hot articles are free to access for four weeks (following a simple registration for individual users).

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Latest hot articles on selective droplet fusion and bubble control in nanoparticle synthesis

Linas Mazutis and Andrew Griffiths demonstrate a passive approach to selectively fuse droplets in 1:1, 2:1 and 3:1 ratios.  They show that spontaneous droplet coalescence is dependant on the contact time between droplets before coalescence, and coverage by the surfactant.

Selective droplet coalescence using microfluidic systems
Linas Mazutis and Andrew D. Griffiths
Lab Chip, 2012, Advance Article
DOI: 10.1039/C2LC40121E

The second hot article is from Saif A. Khan and Suhanya Duraiswamy, who have come up with a way to avoid the problems caused by the evolution of bubbles of H2 in the synthesis of nanoparticles on chips.  They inject a stream of N2 gas into the microfluidic channels, which captures the H2 before it can reach the threshold for bubble nucleation and have shown the method works for the synthesis of ultra-small gold nanocrystals.

Controlling bubbles using bubbles—microfluidic synthesis of ultra-small gold nanocrystals with gas-evolving reducing agents
Saif A. Khan and Suhanya Duraiswamy
Lab Chip, 2012, Advance Article
DOI: 10.1039/C2LC21198J

Both these papers are free to access for 4 weeks following a simple registration for individual users.

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Hot articles on screening antibiotic toxicity, commercializing point-of-care diagnostics & on-bead expression of recombinant proteins

We’re almost ready to wrap up for Easter here, but we have a few treats in the form of free* hot articles for you before we go!

Representation of a microfluidic platform for screening toxic antibiotic cocktailsFirst is a hot article from Piotr Garstecki and co-workers on their microfluidic device to screen hundreds of different cocktails of antibiotics to find combinations that will prove lethal to bacteria and overcome resistance:

Rapid screening of antibiotic toxicity in an automated microdroplet system
Krzysztof Churski,  Tomasz S. Kaminski,  Slawomir Jakiela,  Wojciech Kamysz,  Wioletta Baranska-Rybak,  Douglas B. Weibel and Piotr Garstecki
Lab Chip, 2012, 12, 1629-1637
DOI: 10.1039/C2LC21284F

Next is a Critical Review from Samuel Sia and colleagues on the achievements and challenges in commercialising microfluidic point-of-care diagnostics devices, with advice based on their own experiences:

Commercialization of microfluidic point-of-care diagnostic devices Point-of-care microfluidics
Curtis D. Chin,  Vincent Linder and Samuel K. Sia
Lab Chip, 2012, Advance Article
DOI: 10.1039/C2LC21204H

And the final hot article is from Dong-Myung Kim and colleagues who have developed a new method for expression and capture of recombinant proteins on the same bead:

On-bead expression of recombinant proteins in an agarose gel matrix coated on a glass slide
Kyung-Ho Lee,  Ka-Young Lee,  Ju-Young Byun,  Byung-Gee Kim and Dong-Myung Kim
Lab Chip, 2012, 12, 1605-1610
DOI: 10.1039/C2LC21239K

Happy reading!

* Articles are free to access for individual users following a simple registration process

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LOC issue 8 available now

Issue 8 is now available online, and the front cover features a HOT communication from Sungyoung Choi, Jeffrey M. Karp and Rohit Karnik at the Massachusetts Institute of Technology, Cambridge, USA. The article demonstrates the group’s use of deterministic cell rolling to sort cells in a label-free, gentle manner.

Cell sorting by deterministic cell rolling
Sungyoung Choi, Jeffrey M. Karp and Rohit Karnik
DOI: 10.1039/C2LC21225K

The inside front cover features a HOT paper from Chang Lu and colleagues, which outlines the group’s use of total internal reflection fluorescence flow cytometry (TIRF-FC) to quantitatively measure quantum dot uptake into cells at a single cell level.

Quantitative measurement of quantum dot uptake at the cell population level using microfluidic evanescent-wave-based flow cytometry
Jun Wang, Yihong Zhan, Ning Bao and Chang Lu
DOI: 10.1039/C2LC21298F

Other HOT papers in the issue include:

Microfluidics meets soft layer-by-layer films: selective cell growth in 3D polymer architectures
Narayanan Madaboosi, Katja Uhlig, Stephan Schmidt, Magnus S. Jäger, Helmuth Möhwald, Claus Duschl and Dmitry V. Volodkin
DOI: 10.1039/C2LC40058H

Gene-Z: a device for point of care genetic testing using a smartphone
Robert D. Stedtfeld, Dieter M. Tourlousse, Gregoire Seyrig, Tiffany M. Stedtfeld, Maggie Kronlein, Scott Price, Farhan Ahmad, Erdogan Gulari, James M. Tiedje and Syed A. Hashsham
DOI: 10.1039/C2LC21226A

DNA electrophoresis in a nanofence array
Sung-Gyu Park, Daniel W. Olson and Kevin D. Dorfman
DOI: 10.1039/C2LC00016D

Also in this issue is the ninth instalment of our Acoustofluidics series – this issue’s article focuses on the design, construction and applications of planar resonant devices for acoustic manipulation of particles and cells.

Acoustofluidics 9: Modelling and applications of planar resonant devices for acoustic particle manipulation
Peter Glynne-Jones, Rosemary J. Boltryk and Martyn Hill
DOI: 10.1039/C2LC21257A

Finally, this issue sees the start of a new Focus article series from Tony Jun Huang that will discuss bio-related issues that impact on lab on a chip and microfluidic research. The first article focuses on microfluidic devices for diagnostics in the developing world.

Microfluidic diagnostics for the developing world
Xiaole Mao and Tony Jun Huang
DOI:10.1039/C2LC90022J

Read the rest of issue 8 now!

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New hot articles on cargo-lifting for microfluidics, mimicking cell environments and ‘fluidic batteries’

C2LC21301J graphical abstractGabriel Loget and Alexander Kuhn report for the first time the vertical propulsion of conducting beads in liquid filled capillaries by bipolar electrochemistry. Using a conical capillary a Yo-Yo type motion can be induced, making the concept then useful for cargo-lifting and of potential interest for microfluidic applications in lab-on-a-chip devices.

Bipolar electrochemistry for cargo-lifting in fluid channels
Gabriel Loget and Alexander Kuhn
DOI: 10.1039/C2LC21301J

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C2LC21117C graphical abstractPhilip LeDuc and colleagues from Carnegie Mellon University report the development of a minimal-profile, three-dimensional (MP3D) experimental microdevice which confines cells to a single focal plane, allowing observation with conventional epifluorescent microscopy.

Three-dimensional microfiber devices that mimic physiological environments to probe cell mechanics and signaling
Warren C. Ruder, Erica D. Pratt, Sasha Bakhru, Metin Sitti, Stefan Zappe, Chao-Min Cheng, James F. Antaki and Philip R. LeDuc
DOI: 10.1039/C2LC21117C

C2LC40126F graphical abstract

Scott Phillips and colleagues at Pennsylvania State University describe the first paper-based microfluidic device that is capable of generating its own power when a sample is added to the device. The microfluidic device contains galvanic cells (“fluidic batteries”) integrated directly into the microfluidic channels.

“Fluidic batteries” as low-cost sources of power in paper-based microfluidic devices
Nicole K. Thom, Kimy Yeung, Marley B. Pillion and Scott T. Phillips
DOI: 10.1039/C2LC40126F

All our hot articles are free to access for four weeks (following a simple registration for individual users).

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Lab on a Chip HOT articles

Read on for a quick round up of four HOT articles published recently in Lab on a Chip:

Sung-Gyu Park and colleagues from the University of Minnesota present a nanofence array for DNA electrophoresis that requires no direct-write nanopatterning.

DNA electrophoresis in a nanofence array
Sung-Gyu Park, Daniel W. Olson and Kevin D. Dorfman
DOI: 10.1039/C2LC00016D

Narayanan Madaboosi et al. demonstrate the micropatterning of soft films using a microfluidic device for selective cell growth.

Microfluidics meets soft layer-by-layer films: selective cell growth in 3D polymer architectures
Narayanan Madaboosi, Katja Uhlig, Stephan Schmidt, Magnus S. Jäger, Helmuth Möhwald, Claus Duschl and Dmitry V. Volodkin
DOI: 10.1039/C2LC40058H

Mira T. Guo et al. at Harvard University present a critical review focusing on droplet microfluidics, and the advantages offered for biological assays.

Droplet microfluidics for high-throughput biological assays
Mira T. Guo, Assaf Rotem, John A. Heyman and David A. Weitz
DOI: 10.1039/C2LC21147E

Eugene J. Lim and colleagues demonstrate the use of particle trajectory analysis to observe the inertial focusing behaviour of a range of particles and cells (including prostate cancer cells) in blood.

Visualization of microscale particle focusing in diluted and whole blood using particle trajectory analysis
Eugene J. Lim, Thomas J. Ober, Jon F. Edd, Gareth H. McKinley and Mehmet Toner
DOI: 10.1039/C2LC21100A

These HOT articles are free to access for the next four weeks (following a simple registration for individual users), so why not take a look?

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Our series on acoustofluidics is growing!

We’ve now published the 8th article is our Acoustofluidics series: Applications of acoustophoresis in continuous flow microsystems.  This tutorial focuses on different applications of continuous flow microfluidic acoustic standing wave manipulation such as cell and particle concentration, separation and fractionation.

If you’ve missed any of the previous articles take a look at the web collection here.

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LOC Issue 7 available now

Issue & front coverIssue 7 is now available online, and the outside front cover features a HOT article by Aldo Jesorka and colleagues at Chalmers University of Technology, describing their use of a hydrodynamically confined microflow pipette as an optimized non-contact open-volume microfluidic tool, to overcome the common restriction of using closed microchannels.

A multifunctional pipette
Alar Ainla, Gavin D. M. Jeffries, Ralf Brune, Owe Orwar and Aldo Jesorka
DOI: 10.1039/C2LC20906C

Issue 7 inside front coverThe inside front cover is a Critical Review by David Beebe et al., discussing the use of PDMS and polystyrene by researchers working at the interface of microfluidics and cell biology research:

Engineers are from PDMS-land, Biologists are from Polystyrenia
Erwin Berthier, Edmond W. K. Young and David Beebe
DOI: 10.1039/C2LC20982A

Another HOT article in this issue is by Serge G. Lemay and colleagues at the MESA+ Institute for Nanotechnology, University of Twente, reporting on their study of the response time of nanofluidic thin-layer cells as electrochemical sensors, and suggested means of improving that time.

Response time of nanofluidic electrochemical sensors
Shuo Kang, Klaus Mathwig and Serge G. Lemay
DOI: 10.1039/C2LC21104A

This issue also features the latest article in our Acoustofluidics series, by Thomas Laurell and colleagues at Lund University, Applications of acoustophoresis in continuous flow microsystems; and our latest Research Highlights from Ali Khademhosseini and colleagues.

Read the rest of Issue 7 here


As usual, all our HOT articles are free to access for four weeks (following a simple registration for individual users).

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