Yu et al., Anal. Methods, 2013, Advance Article
Mutation, apoptosis and cancer can develop from damaged DNA, however the cell produces proteins capable of repairing DNA to prevent these dangerous effects. One of these proteins produced by cells, 8-oxoG DNA glycosylase/AP lyase (hOGG1), targets DNA lesions caused by reactive oxygen species. Typically studying this glycosylase requires large instrumentation, such as HPLC, or radioactive labeling. Ru-Qin Yu and coworkers at Hunan University developed a system, where the hOGG1 cleaves at the mismatch site on a DNA strand, and releases a DNAzyme mimetic, which produces a colored signal. This rapid and sensitive colorimetric assay measured the activity of hOGG1 for a range of concentrations, with an affordable DNA probe, and user-friendly instrumentation.
This paper will be free to read until Nov 30th.
A novel DNAzyme-based colorimetric assay for the detection of hOGG1 activity with lambda exonuclease cleavage
Shu-Cheng Liu, Hui-Wang Wu, Jian-hui Jiang, Guo-Li Shen and Ru-Qin Yu
Anal. Methods, 2013, Advance Article
DOI: 10.1039/C2AY26018B
