Point-of-Care Paper Based Rapid Allergy Diagnostics

Matthew J. Baker is a web-writer for Analyst. He is currently Senior Lecturer in Toxicology and Analytical Chemistry at the University of Central Lancashire (UCLan), UK

Recent surveys from Allergy UK have shown that the rates of allergy are increasing throughout the world, affecting up to 30-35 % of people at some point during their lives, in some cases with fatal consequences. In vitro testing of specific Immunoglobin E (IgE) reactivities is thought to be able to significantly improve diagnostic accuracy and management in primary care. IgE plays a significant role in allergic conditions. Current methods for the sensitive and accurate detection of specific IgE reactivities are expensive; require significant sample preparation and investment in equipment. There is a need to provide a cheap, rapid, sensitive and specific test for IgE reactivities.

In a recent HOT article, a group of Swedish and Dutch Scientists describe a paper based lateral flow microarray for rapid allergy diagnostics. One of the authors, Jesper Gantelius of the Science for Life Laboratory, highlights “There is a rapid worldwide increase in atopic disorders and asthma without a clear understanding of the underlying cause. We have developed a paper-based microarray assay that could be used to rapidly and cost-efficiently evaluate a large number of specific IgE sensitisation’s in a primary care setting. The device may come to aid doctors in the personalised management of individual patients, as well as allow public health bodies to gain a better insight into the rapid increase of allergy-related conditions.”

The group, led by Prof. Helene Andersson Svahn, describe exciting research that was able to detect the responses of 15 specific IgE responses from 35 clinical serum samples with an inter-assay variability of 12 % revealing good general agreement with a current multiplexed array-based methodology. Interestingly this process is fully scalable and all that is required for data feedback is a consumer-grade flatbed scanner or smartphone camera enabling point-of-care diagnostics.

A lateral flow paper microarray for rapid allergy point of care diagnostics

Thiruppathiraja ChinnasamyLoes I. Segerink, Mats Nystrand, Jesper Gantelius and Helene Andersson Svahn

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Analytical Tools for Cutting-edge Metabolomics – April 30th

Analytical Tools for Cutting-edge Metabolomics – a joint meeting of the Analytical Division of the RSC and the International Metabolomics Society

Analytical chemistry has been one of the driving forces behind the development of metabolomics research over the past decade. The conference will bring together exceptional scientists for a program consisting of plenary and invited talks, posters, as well as an oral session devoted to early career researchers. It will be an excellent opportunity for analytical chemists to learn more about metabolomics and its application, and for metabolomics scientists to improve their knowledge of cutting-edge bioanalytical tools.

More information can be found here.

Representatives of the Royal Society of Chemistry will be in attendance to answer your questions about the Analytical Division, and the Analytical Journals Portfiolio in Publishing. We hope we see you there.

Date: 30 April 2014, 09:30 – 16:45
Location: Burlington House, London
Contact: Mr Brian Woodget
Email: bwoodget1@sky.com
Tel: 01438 880286


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HOT articles in Analyst

Take a look at our new HOT articles just published in Analyst and free for you for the next couple of weeks: Graphical abstract: Electrochemiluminescence resonance energy transfer between graphene quantum dots and gold nanoparticles for DNA damage detection

Electrochemiluminescence resonance energy transfer between graphene quantum dots and gold nanoparticles for DNA damage detection
Qian Lu, Wei Wei, Zhenxian Zhou, Zhixin Zhou, Yuanjian Zhang and Songqin Liu
Analyst, 2014, Advance Article
DOI: 10.1039/C4AN00020J, Paper

A lateral flow paper microarray for rapid allergy point of care diagnostics
Thiruppathiraja Chinnasamy, Loes I. Segerink, Mats Nystrand, Jesper Gantelius and Helene Andersson Svahn
Analyst, 2014, Advance Article
DOI: 10.1039/C3AN01806G, Paper

Nanoparticles in sensing applications: on what timescale do analyte species adsorb on the particle surface?
Enno Kätelhön and Richard G. Compton
Analyst, 2014, Advance Article
DOI: 10.1039/C4AN00363B, Paper

The use of EEM fluorescence data and OPLS/UPLS-DA algorithm to discriminate between normal and cancer cell lines: a feasibility study
Ana Carolina de Oliveira Neves, Raimundo Fernandes de Araújo Júnior, Ana Luiza Cabral de Sá Leitão Oliveira, Aurigena Antunes de Araújo and Kássio Michell Gomes de Lima Graphical abstract: A lateral flow paper microarray for rapid allergy point of care diagnostics
Analyst, 2014, Advance Article
DOI: 10.1039/C4AN00296B, Paper

Enhancing permanganate chemiluminescence detection for the determination of glutathione and glutathione disulfide in biological matrices
Zoe M. Smith, Jessica M. Terry, Neil W. Barnett, Laura J. Gray, Dean J. Wright and Paul S. Francis
Analyst, 2014, Advance Article
DOI: 10.1039/C4AN00255E, Paper

Mass spectrometric investigation of the role of the linking polypeptide chain in DNA polymerase I
Taeho Yeom, Jungyoon Lee, Seonghyun Lee, Sunah Kang, Kyung Rok Kim, Byungwoo Han, Hyun Soo Lee and Kyubong Jo
Analyst, 2014, Advance Article
DOI: 10.1039/C4AN00107A, Paper

Optimizing detection of RDX vapors using designed experiments for remote sensing
Robert G. Ewing, Alejandro Heredia-Langner and Marvin G. WarnerGraphical abstract: Nanoparticles in sensing applications: on what timescale do analyte species adsorb on the particle surface?
Analyst, 2014, Advance Article
DOI: 10.1039/C4AN00125G, Paper

Mixed hydrogel bead-based tumor spheroid formation and anticancer drug testing
Yaolei Wang and Jinyi Wang
Analyst, 2014, Advance Article
DOI: 10.1039/C4AN00015C, Paper

Carbon nanobelts as a novel sensing platform for fluorescence-enhanced DNA detection
Xuping Sun, Zhicai Xing, Rui Ning, Abdullah M. Asiri and Abdullah Y. Obaid
Analyst, 2014, Advance Article
DOI: 10.1039/C3AN02364H, Communication

To view these article for free, please register for a free RSC account here

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Poster prize winners at the 11th Uppsala Conference

Congratulations to the Analyst Poster Prize winners at the recent 11th Uppsala Conference.

 Christopher Andrew Wootton
University of Warwick, UK

 Heidelinde Glasner
University of Innsbruck, Austria

    
 

Owen Samuel Skinner
Northwestern University, USA

 

 

Well done Christopher, Heidelinde and Owen!

Thanks to the conference organisers Dr Thomas Müller and Dr Kathrin Breuker as well as Editorial Board Member Professor Evan Williams who presented the poster prizes. We hope you enjoyed the 11th Uppsala Conference on Electron Capture and Transfer Dissociation (UppCon 2014) held in Obergurgl, Austria.

   
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Analytical tools made from ordinary office paper

Hazel Neighbour writes about a HOT Analyst article in Chemistry World

Commonplace equipment can turn office paper into cheap, portable and disposable electrochemical devices that are ideal for using in remote locations.

The simple sensors from William de Araujo and Thiago da Paixão at the University of São Paulo, Brazil combine silver ink with ordinary office paper. Office paper is 97% cheaper than the chromatography paper commonly used in cutting-edge paper-based sensors.

To read the full article by Hazel Neighbour, please go to Chemistry World.

Fabrication of disposable electrochemical devices using silver ink and office paper
William Araujo and Thiago Regis Longo Cesar Paixão
Analyst, 2014, Accepted Manuscript
DOI: 10.1039/C4AN00097H, Paper

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HOT articles in Analyst

Take a look at our new HOT articles just published in Analyst and free for you for the next couple of weeks: Graphical abstract: Improving the fluorescence detection limit with positively charged carbon nanostructures as a low background signal platform

Recent advances in sample preparation techniques to overcome difficulties encountered during quantitative analysis of small molecules from biofluids using LC-MS/MS
Caroline Bylda, Roland Thiele, Uwe Kobold and Dietrich A. Volmer
Analyst, 2014, Advance Article
DOI: 10.1039/C4AN00094C, Minireview

Convenient detection of the thiol functional group using H/D isotope sensitive Raman spectroscopy
Sabyasachi Bandyopadhyay and Abhishek Dey
Analyst, 2014, Advance Article
DOI: 10.1039/C3AN02166A, Communication

Improving the fluorescence detection limit with positively charged carbon nanostructures as a low background signal platform
Xiulan He, Li Zhang, Hetong Qi, Ping Yu, Junjie Fei and Lanqun Mao
Analyst, 2014, Advance Article
DOI: 10.1039/C4AN00124A, Communication

Femtoliter-scale separation and sensitive detection of nonfluorescent samples in an extended-nano fluidic device
Hisashi Shimizu, Kazuma Mawatari and Takehiko Kitamori
Analyst, 2014, Advance Article
DOI: 10.1039/C3AN02353B, Paper

To view these article for free, please register for a free RSC account here

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HOT articles in Analyst

Take a look at our new HOT articles just published in Analyst and free for you for the next couple of weeks: Graphical abstract: Viral plaque analysis on a wide field-of-view, time-lapse, on-chip imaging platform

Viral plaque analysis on a wide field-of-view, time-lapse, on-chip imaging platform
Chao Han and Changhuei Yang
Analyst, 2014, Advance Article
DOI: 10.1039/C3AN02323K, Paper

IR action spectroscopy shows competitive oxazolone and diketopiperazine formation in peptides depends on peptide length and identity of terminal residue in the departing fragment
L. J. Morrison, J. Chamot-Rooke and V. H. Wysocki
Analyst, 2014, Advance Article
DOI: 10.1039/C4AN00064A, Paper

Energy-resolved collision-induced dissociation pathways of model N-linked glycopeptides: implications for capturing glycan connectivity and peptide sequence in a single experiment
Venkata Kolli and Eric D. Dodds
Analyst, 2014, Advance Article
DOI: 10.1039/C3AN02342G, Paper

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HOT articles in Analyst

Take a look at our new HOT articles just published in Analyst and free for you for the next couple of weeks: Graphical abstract: Live-cell vibrational imaging of choline metabolites by stimulated Raman scattering coupled with isotope-based metabolic labeling

Live-cell vibrational imaging of choline metabolites by stimulated Raman scattering coupled with isotope-based metabolic labeling
Fanghao Hu, Lu Wei, Chaogu Zheng, Yihui Shen and Wei Min
Analyst, 2014, Advance Article
DOI: 10.1039/C3AN02281A, Communication

Chip based single cell analysis for nanotoxicity assessment
Pratikkumar Shah, Ajeet Kaushik, Xuena Zhu, Chengxiao Zhang and Chen-Zhong Li
Analyst, 2014, Advance Article
DOI: 10.1039/C3AN02280C, Minireview

Chemical imaging of artificial fingerprints by desorption electro-flow focusing ionization mass spectrometry
Thomas P. Forbes and Edward Sisco
Analyst, 2014, Advance Article
DOI: 10.1039/C4AN00172A, Communication

Binding assay for low molecular weight analytes based on reflectometry of absorbing molecules in porous substrates
Milena Stephan, Corinna Kramer, Claudia Steinem and Andreas Janshoff
Analyst, 2014, Advance Article
DOI: 10.1039/C4AN00009A, Paper

Quantized double layer charging of Au130(SR)50 nanomoleculesGraphical abstract: Chemical imaging of artificial fingerprints by desorption electro-flow focusing ionization mass spectrometry
Vijay Reddy Jupally, Jacob G. Thrasher and Amala Dass
Analyst, 2014, Advance Article
DOI: 10.1039/C3AN02204H, Communication

Robotic plasma probe ionization mass spectrometry (RoPPI-MS) of non-planar surfaces
Rachel V. Bennett, Ezequiel M. Morzan, Jacob O. Huckaby, María Eugenia Monge, Henrick I. Christensen and Facundo M. Fernández
Analyst, 2014, Advance Article
DOI: 10.1039/C4AN00277F, Communication

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Kick Off and the Power of Twitter

Matthew J. Baker is a web-writer for Analyst. He is currently Senior Lecturer in Toxicology and Analytical Chemistry at the University of Central Lancashire (UCLan), UK

I was waiting for a train on a cold and rainy Friday morning in February at Preston train station (this will be a shock for those that know the Preston climate as it is actually subtropical) and tweeting from @ChemistryBaker to my colleagues @RoyGoodacre and @AlexHenderson00 about the upcoming kick off meeting of the Clinical Infrared and Raman Spectroscopy network. Analyst (@analystrsc) picked this tweet up and through a few tweets back and forth agreed to host a blog on the subject and here it is; to show a different side of research and the way a fledgling network starts and, hopefully, becomes successful. Success for us is closely linked to a clinical impact that would have a massive positive effect on many many people’s lives.

The UK is a world leader in the field of vibrational biomedical spectroscopy with excellent research being performed by many groups based in our academic and governmental research systems. Diagnostic and prognostic tools based on these technologies have the potential to revolutionise our clinical systems leading to improved patient outcome, a more efficient healthcare service and significant economic savings. However, despite these strong reasons and the research that has occurred to date, this technology has not truly made the jump into the clinic. There was a need to bring many researchers, clinicians and industrialists together in order to provide a critical mass that will overcome these clinical implementation barriers.

It was for these reasons, that in 2012, several of us got together to speak about the possibility of developing a bid for an EPSRC network grant. Importantly network grants provide no money for research projects rather funding to enable collaborative multidisciplinary meetings targeted to tackle problems and drive a field forwards. Luckily EPSRC agreed (thank you EPSRC) that our bid, plan and field deserved funding and the EPSRC Clinical Infrared and Raman SPECtroscopy (CLIRSPEC) network was set up.

Even though the meeting that February day was long and actually quite tiring it was also fun and welcoming. Our field is known to be a supportive field. Essentially the aims of the meeting was to get people together to discuss and finalise our plans to overcome the challenges we identified, agree how we assess bids to the training fund for PhDs and PDRAs, conference locations and summer school locations.  The main aim was to ask already overworked people to do more work for free, which thankfully they agreed to.

The work of the network will be achieved through six working parties that aim to overcome the barriers that challenge our field:

1)   To develop our understanding of the interaction of light with clinical samples

2)   To develop internationally recognized protocols for the preparation of cells, tissue and biofluids for clinical spectroscopy

3)   To provide evidence of the power of spectroscopy for use in the clinical arena

4)   To determine the requirements of instrumentation suitable for use in the clinic

5)   To develop strategies for pre-processing and statistical analysis in clinical spectroscopy

6)   To develop inter-group data sharing protocols and portal

I would like to use this first blog to ask if there are any clinicians, scientists or industrialists out there who feel they can contribute to the cause. We currently have international and national partners and would welcome more. If you feel you can contribute then please visit our website. We do not want to be a closed shop but rather harness the power of many to achieve our aims.

Until the next time…

Please find links to recent papers below that describe the research currently on-going in the field to demonstrate the application of spectroscopy to the clinical environment as well as tackling the current research challenges.

The inherent problem of transflection-mode infrared spectroscopic microscopy and the ramifications for biomedical single point and imaging applications

Paul Bassan, Joe Lee, Ashwin Sachdeva, Juliana Pissardini, Konrad M. Dorling, John

S. Fletcher, Alex Henderson and Peter Gardner
Analyst, 2013,138, 144-157

A comparison of Raman, FTIR and ATR-FTIR micro spectroscopy for imaging human skin tissue sections
S. M. Ali, F. Bonnier, H. Lambkin, K. Flynn, V. McDonagh, C. Healy, T. C. Lee, F. M. Lyng and H. J. Byrne
Anal. Methods, 2013,5, 2281-2291

Effect of substrate choice and tissue type on tissue preparation for spectral histopathology by Raman microspectroscopy
Leanne M. Fullwood, Dave Griffiths, Katherine Ashton, Timothy Dawson, Robert W. Lea, Charles Davis, Franck Bonnier, Hugh J. Byrne and Matthew J. Baker
Analyst, 2014,139, 446-454

Identification of different subsets of lung cells using Raman microspectroscopy and whole cell nucleus isolation
Jacek K. Pijanka, Nicholas Stone, Abigail V. Rutter, Nicholas Forsyth, Ganesh D. Sockalingum, Ying Yang and Josep Sulé-Suso
Analyst, 2013,138, 5052-5058

ATR-FTIR spectroscopic imaging: recent advances and applications to biological systems
Sergei G. Kazarian and K. L. Andrew Chan
Analyst, 2013,138, 1940-1951

Illuminating disease and enlightening biomedicine: Raman spectroscopy as a diagnostic tool

David I. Ellis, David P. Cowcher, Lorna Ashton, Steve O’Hagan and Royston Goodacre
Analyst, 2013,138, 3871-3884

Simultaneous detection and quantification of three bacterial meningitis pathogens by SERS
Kirsten Gracie, Elon Correa, Samuel Mabbott, Jennifer A. Dougan, Duncan Graham, Royston Goodacre and Karen Faulds
Chem. Sci., 2014,5, 1030-1040

Resonance Raman microscopy in combination with partial dark-field microscopy lights up a new path in malaria diagnostics
Bayden R. Wood, Antje Hermelink, Peter Lasch, Keith R. Bambery, Grant T. Webster, Mehdi Asghari Khiavi, Brian M. Cooke, Samantha Deed, Dieter Naumann and Don McNaughton
Analyst, 2009,134, 1119-1125

Extracting biological information with computational analysis of Fourier-transform infrared (FTIR) biospectroscopy datasets: current practices to future perspectives
Júlio Trevisan, Plamen P. Angelov, Paul L. Carmichael, Andrew D. Scott and Francis L. Martin
Analyst, 2012,137, 3202-3215

Emerging concepts in deep Raman spectroscopy of biological tissue
Pavel Matousek and Nicholas Stone
Analyst, 2009,134, 1058-1066

Vibrational spectroscopy: a clinical tool for cancer diagnostics
Catherine Kendall, Martin Isabelle, Florian Bazant-Hegemark, Joanne Hutchings, Linda Orr, Jaspreet Babrah, Rebecca Baker and Nicholas Stone
Analyst, 2009,134, 1029-1045

Also please see the latest themed issue on Optical Diagnostics

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SPEC 2014 -Shedding New Light on Disease- submissions open!

logo

Submissions now open! Abstracts must be submitted online via the abstract registration form.

Deadline for submission is 15th May 2014.

The aim of this international conference series is to bring together clinicians and scientists who have joined forces in the quest for novel biomedical applications of Infrared and Raman spectroscopy to improve patient care. Recent advances in the biological sciences and medicine have led to an increasing demand for real time and minimally invasive chemical and structural information on biological materials. Due to its unique fingerprinting capability, vibrational spectroscopy plays a significant role in histopathology, cytology, biopsy targeting, surgical targets, treatment monitoring and drug studies. This, the 8th in the series, aims to highlight further advances in state of the art and emerging biomedical applications of Vibrational Spectroscopy, while critically reviewing the challenges in the context of other emerging technologies.

Date: 17-22 August 2014
Location: Krakow, Poland
Contact: Katarzyna Marzec
Email: marzec@chemia.uj.edu.pl
Website

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