Organic & Biomolecular Chemistry Blog

Deazapurines are bicyclic heterocycles derived from purine where one nitrogen atom is replaced by a carbon. Although they have been widely studied in recent years, the mechanistic understanding of the biosynthesis of 7-deazapurines has always been intriguing.

There is a question that inevitably arise when looking at the biosynthetic pathway of deazapurines: Where does N-7 go? 

Now, Professor Moody and his group at University of Nottingham have solved this mystery by using a doubly labelled purine-adenine and following it up by NMR spectroscopy and mass spectrometry.
Their conclusions are supported and support a recent study published in Biochemistry.

If you want to find out more about the fate of this nitrogen and the mechanistic understanding of the biosynthesis of deazapurines, read this HOT paper which is free to access until 16th March.

7-Deazapurine biosynthesis: NMR study of toyocamycin biosynthesis in Streptomyces rimosus using 2-13C-7-15N-adenine
Ugo Battaglia, Jed E. Long, Mark S. Searle and Christopher J. Moody
Org. Biomol. Chem., 2011
DOI: 10.1039/C0OB01054E

Lantibiotics (and there is no spelling mistake) are a potential new class of antibiotics for clinical applications and food preservation. Their drawback is however their inability to penetrate the outer membranes of Gram-negative bacteria.

John Vederas and colleagues, at University of Alberta in Canada, try to overcome this problem by developing new ‘siderophores – transporters’ able to deliver lantibiotics to the targeted bacteria.

If you want to find out more about what happened next and the scope and limitation of siderophore-mediated drug transport for the development of new antibiotics download this HOT article which is free to access until 8th March.

Chemical synthesis and biological evaluation of gallidermin-siderophore conjugates
Sabesan Yoganathan, Clarissa S. Sit and John C. Vederas
Org. Biomol. Chem., 2011
DOI: 10.1039/C0OB00846J

Nature has been giving organic chemists inferiority complexes for many, many years, so it is always good to see a neat piece of synthesis for a tricky natural product.

The structure of viridenomycin – an antibiotic with anti-tumor activity – has been known for 20 years, but attempts to synthesise it thus far have failed due to its complex structure and inherent instability. Now, Andy Whiting and colleagues from Durham University have synthesised fragments suitable for constructing a particularly unstable part of viridenomycin via a series of cross-coupling reactions.

They focused on the northern polyene fragment, synthesising several analogues with better isometric ratios and increased stability towards photoisomerisation than the original tetraene fragment.  The authors hope that their method may help to achieve the total synthesis of this valuable complex molecule.

Read how they did it online – the article is free to access for 4 weeks!

Studies towards the synthesis of the northern polyene of viridenomycin and synthesis of Z-double bond analogues
Jonathan P. Knowles, Victoria E. O′Connor and Andrew Whiting
Org. Biomol. Chem., 2011, Advance Article
DOI: 10.1039/C0OB00977F

Organic azides are considered to be amongst the most hazardous chemicals and therefore not amongst the most popular within  synthetic chemistry communities. In organic chemistry they are commonly used as a way to introduce an amine group, which makes them key compounds for synthetic chemists.

Steven Ley, Ian Baxendale and their group at University of Cambridge, describe in these back to back papers the development of a flow process for the synthesis of alkyl and aryl azides in high conversions. One of the key features of this flow procedure is the introduction of a new monolithic triphenylphosphine reagent that allows the use of triphenylphosphine in flow to provide high purity products without the need of further purification steps.

They also describe a general protocol for the in-line purification of the intermediates. They incorporate the azide synthesis and purification process into a multistep flow sequence to generate a collection of aminocyanotriazoles in a fully automated fashion.

Now you can read these very interesting and HOT papers which are free to access until the 8th March.

Download both papers here.

Flow synthesis of organic azides and the multistep synthesis of imines and amines using a new monolithic triphenylphosphine reagent
Catherine J. Smith, Christopher D. Smith, Nikzad Nikbin, Steven V. Ley and Ian R. Baxendale
Org. Biomol. Chem., 2011
DOI: 10.1039/C0OB00813C

A fully automated, multistep flow synthesis of 5-amino-4-cyano-1,2,3-triazoles
Catherine J. Smith, Nikzad Nikbin, Steven V. Ley, Heiko Lange and Ian R. Baxendale
Org. Biomol. Chem., 2011
DOI: 10.1039/C0OB00815J

T. cruzi, the causative agent of Chagas disease, relies on its enzyme trans-sialidase for part of its infectivity. Robert Field et al., from several collaborating institutions worldwide, have studied this enzyme, highlighting the wide range of structures and functionalities that it can accommodate. This study has demonstrated important features for potential inhibitor design (a therapeutic target for Chagas’ disease) and also opens up possibilities for using the versatility of this enzyme more generally as a catalyst for α-(2→3)-sialylglycoconjugate synthesis.

This HOT article is now free to access until 22nd February.

Probing the acceptor substrate binding site of Trypanosoma cruzi trans-sialidase with systematically modified substrates and glycoside libraries
Jennifer A. Harrison, K. P. Ravindranathan Kartha, Eric J. L. Fournier, Todd L. Lowary, Carles Malet, Ulf J. Nilsson, Ole Hindsgaul, Sergio Schenkman, James H. Naismith and Robert A. Field
Org. Biomol. Chem., 2011, Advance Article
DOI: 10.1039/C0OB00826E, Paper

PBDs (pyrrolo[2,1-c][1,4]benzodiazepines) and their interaction with DNA is an area of active research.

PBDs belong to a family of biologically active an DNA-interactive antibiotics having unique mechanism of action compared with other DNA-binding agents. Up until now, there have been several reports in literature suggesting that PBD-DNA adduct formation might be reversible; however, no evidence of this reversibility had been reported.

For the first time, in this paper, David Thurston and colleagues at the School of Pharmacy at University of London, investigate the adduct formation of PBDs with DNA and its reversibility using HPLC/MS methodology and polarised light spectroscopy.

Read about some of their findings in this OBC HOT paper which is free to access until the 22nd February.

Observation of the reversibility of a covalent pyrrolobenzodiazepine (PBD) DNA adduct by HPLC/MS and CD spectroscopy
Khondaker M. Rahman, Colin H. James and David E. Thurston
Org. Biomol. Chem., 2011, Advance Article
DOI: 10.1039/C0OB00762E, Paper