PBDs (pyrrolo[2,1-c][1,4]benzodiazepines) and their interaction with DNA is an area of active research.
PBDs belong to a family of biologically active an DNA-interactive antibiotics having unique mechanism of action compared with other DNA-binding agents. Up until now, there have been several reports in literature suggesting that PBD-DNA adduct formation might be reversible; however, no evidence of this reversibility had been reported.
For the first time, in this paper, David Thurston and colleagues at the School of Pharmacy at University of London, investigate the adduct formation of PBDs with DNA and its reversibility using HPLC/MS methodology and polarised light spectroscopy.
Read about some of their findings in this OBC HOT paper which is free to access until the 22nd February.
Observation of the reversibility of a covalent pyrrolobenzodiazepine (PBD) DNA adduct by HPLC/MS and CD spectroscopy
Khondaker M. Rahman, Colin H. James and David E. Thurston
Org. Biomol. Chem., 2011, Advance Article
DOI: 10.1039/C0OB00762E, Paper
