Archive for 2012

Reminder: Emerging Investigators themed issue

Emerging Investigators 2011 The deadline is fast approaching for Lab on a Chip‘s Emerging Investigators themed issue, guested edited by Professors Amy Herr and Aaron Wheeler.

If you plan to submit an article, we advise you to read our guidelines for authors prior to submission. Submissions should be made through http://mc.manuscriptcentral.com/lc, noting in the ‘Comments to the Editor’ that the article is intended for the Emerging Investigators 2012 themed issue.

Please note that the submission deadline for this issue is extremely strict to tie in with the microTAS conference in October this year, therefore articles submitted after the 30th April deadline may not be included in the themed issue.  Submissions before this date are welcomed.

If you have any questions about your submission to this issue, please do not hesitate to email the Editorial Office.

If you would like more information about the Emerging Investigators themed issue, please read our Call for Papers.

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New YouTube videos

View the new videos on the Lab on a Chip YouTube site below:

Walking nanothermometers: spatiotemporal temperature measurement of transported acidic organelles in single living cells

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Enhanced bile canaliculi formation enabling direct recovery of biliary metabolites of hepatocytes in 3D collagen gel microcavities

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Latest hot articles on selective droplet fusion and bubble control in nanoparticle synthesis

Linas Mazutis and Andrew Griffiths demonstrate a passive approach to selectively fuse droplets in 1:1, 2:1 and 3:1 ratios.  They show that spontaneous droplet coalescence is dependant on the contact time between droplets before coalescence, and coverage by the surfactant.

Selective droplet coalescence using microfluidic systems
Linas Mazutis and Andrew D. Griffiths
Lab Chip, 2012, Advance Article
DOI: 10.1039/C2LC40121E

The second hot article is from Saif A. Khan and Suhanya Duraiswamy, who have come up with a way to avoid the problems caused by the evolution of bubbles of H2 in the synthesis of nanoparticles on chips.  They inject a stream of N2 gas into the microfluidic channels, which captures the H2 before it can reach the threshold for bubble nucleation and have shown the method works for the synthesis of ultra-small gold nanocrystals.

Controlling bubbles using bubbles—microfluidic synthesis of ultra-small gold nanocrystals with gas-evolving reducing agents
Saif A. Khan and Suhanya Duraiswamy
Lab Chip, 2012, Advance Article
DOI: 10.1039/C2LC21198J

Both these papers are free to access for 4 weeks following a simple registration for individual users.

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Hot articles on screening antibiotic toxicity, commercializing point-of-care diagnostics & on-bead expression of recombinant proteins

We’re almost ready to wrap up for Easter here, but we have a few treats in the form of free* hot articles for you before we go!

Representation of a microfluidic platform for screening toxic antibiotic cocktailsFirst is a hot article from Piotr Garstecki and co-workers on their microfluidic device to screen hundreds of different cocktails of antibiotics to find combinations that will prove lethal to bacteria and overcome resistance:

Rapid screening of antibiotic toxicity in an automated microdroplet system
Krzysztof Churski,  Tomasz S. Kaminski,  Slawomir Jakiela,  Wojciech Kamysz,  Wioletta Baranska-Rybak,  Douglas B. Weibel and Piotr Garstecki
Lab Chip, 2012, 12, 1629-1637
DOI: 10.1039/C2LC21284F

Next is a Critical Review from Samuel Sia and colleagues on the achievements and challenges in commercialising microfluidic point-of-care diagnostics devices, with advice based on their own experiences:

Commercialization of microfluidic point-of-care diagnostic devices Point-of-care microfluidics
Curtis D. Chin,  Vincent Linder and Samuel K. Sia
Lab Chip, 2012, Advance Article
DOI: 10.1039/C2LC21204H

And the final hot article is from Dong-Myung Kim and colleagues who have developed a new method for expression and capture of recombinant proteins on the same bead:

On-bead expression of recombinant proteins in an agarose gel matrix coated on a glass slide
Kyung-Ho Lee,  Ka-Young Lee,  Ju-Young Byun,  Byung-Gee Kim and Dong-Myung Kim
Lab Chip, 2012, 12, 1605-1610
DOI: 10.1039/C2LC21239K

Happy reading!

* Articles are free to access for individual users following a simple registration process

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Gut-on-a-Chip in the New Scientist!

The article on a ‘gut-on-a-chip’ from Editorial Board member Don Ingber and colleagues which has been causing some rumblings in the scientific press over the last couple of weeks has made it to the New Scientist.  The article also recently featured as a Nature Research Highlight and is part of one of our forthcoming themed issues – watch this space!

Human gut-on-a-chip inhabited by microbial flora that experiences intestinal peristalsis-like motions and flow
Hyun Jung Kim, Dongeun Huh, Geraldine Hamilton and Donald E. Ingber
DOI: 10.1039/C2LC40074J

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LOC issue 8 available now

Issue 8 is now available online, and the front cover features a HOT communication from Sungyoung Choi, Jeffrey M. Karp and Rohit Karnik at the Massachusetts Institute of Technology, Cambridge, USA. The article demonstrates the group’s use of deterministic cell rolling to sort cells in a label-free, gentle manner.

Cell sorting by deterministic cell rolling
Sungyoung Choi, Jeffrey M. Karp and Rohit Karnik
DOI: 10.1039/C2LC21225K

The inside front cover features a HOT paper from Chang Lu and colleagues, which outlines the group’s use of total internal reflection fluorescence flow cytometry (TIRF-FC) to quantitatively measure quantum dot uptake into cells at a single cell level.

Quantitative measurement of quantum dot uptake at the cell population level using microfluidic evanescent-wave-based flow cytometry
Jun Wang, Yihong Zhan, Ning Bao and Chang Lu
DOI: 10.1039/C2LC21298F

Other HOT papers in the issue include:

Microfluidics meets soft layer-by-layer films: selective cell growth in 3D polymer architectures
Narayanan Madaboosi, Katja Uhlig, Stephan Schmidt, Magnus S. Jäger, Helmuth Möhwald, Claus Duschl and Dmitry V. Volodkin
DOI: 10.1039/C2LC40058H

Gene-Z: a device for point of care genetic testing using a smartphone
Robert D. Stedtfeld, Dieter M. Tourlousse, Gregoire Seyrig, Tiffany M. Stedtfeld, Maggie Kronlein, Scott Price, Farhan Ahmad, Erdogan Gulari, James M. Tiedje and Syed A. Hashsham
DOI: 10.1039/C2LC21226A

DNA electrophoresis in a nanofence array
Sung-Gyu Park, Daniel W. Olson and Kevin D. Dorfman
DOI: 10.1039/C2LC00016D

Also in this issue is the ninth instalment of our Acoustofluidics series – this issue’s article focuses on the design, construction and applications of planar resonant devices for acoustic manipulation of particles and cells.

Acoustofluidics 9: Modelling and applications of planar resonant devices for acoustic particle manipulation
Peter Glynne-Jones, Rosemary J. Boltryk and Martyn Hill
DOI: 10.1039/C2LC21257A

Finally, this issue sees the start of a new Focus article series from Tony Jun Huang that will discuss bio-related issues that impact on lab on a chip and microfluidic research. The first article focuses on microfluidic devices for diagnostics in the developing world.

Microfluidic diagnostics for the developing world
Xiaole Mao and Tony Jun Huang
DOI:10.1039/C2LC90022J

Read the rest of issue 8 now!

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New hot articles on cargo-lifting for microfluidics, mimicking cell environments and ‘fluidic batteries’

C2LC21301J graphical abstractGabriel Loget and Alexander Kuhn report for the first time the vertical propulsion of conducting beads in liquid filled capillaries by bipolar electrochemistry. Using a conical capillary a Yo-Yo type motion can be induced, making the concept then useful for cargo-lifting and of potential interest for microfluidic applications in lab-on-a-chip devices.

Bipolar electrochemistry for cargo-lifting in fluid channels
Gabriel Loget and Alexander Kuhn
DOI: 10.1039/C2LC21301J

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C2LC21117C graphical abstractPhilip LeDuc and colleagues from Carnegie Mellon University report the development of a minimal-profile, three-dimensional (MP3D) experimental microdevice which confines cells to a single focal plane, allowing observation with conventional epifluorescent microscopy.

Three-dimensional microfiber devices that mimic physiological environments to probe cell mechanics and signaling
Warren C. Ruder, Erica D. Pratt, Sasha Bakhru, Metin Sitti, Stefan Zappe, Chao-Min Cheng, James F. Antaki and Philip R. LeDuc
DOI: 10.1039/C2LC21117C

C2LC40126F graphical abstract

Scott Phillips and colleagues at Pennsylvania State University describe the first paper-based microfluidic device that is capable of generating its own power when a sample is added to the device. The microfluidic device contains galvanic cells (“fluidic batteries”) integrated directly into the microfluidic channels.

“Fluidic batteries” as low-cost sources of power in paper-based microfluidic devices
Nicole K. Thom, Kimy Yeung, Marley B. Pillion and Scott T. Phillips
DOI: 10.1039/C2LC40126F

All our hot articles are free to access for four weeks (following a simple registration for individual users).

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LOC article featuring a ‘gut-on-a-chip’ is mentioned in the press

The recent LOC article from Editorial Board member Donald Ingber and colleagues at Harvard University has been selected as a Research Highlight by Nature. The article describes a microfluidic chip that can model the structure and physiology of the human intestinal system, using two microfluidic channels coated with human intestinal epithelial cells. The channels can be used to culture Lactobacillus rhamnosus GG, a common intestinal microbe, and the microdevice has the potential for use in drug development.

The article has also been picked up by several news sites, including Science Daily, Medgadget, Gizmag, CNET Australia and SmartPlanet!

Congratulations to Donald and the rest of the team!

You can read the Nature article online here or go straight to the Lab on a Chip paper:

Human gut-on-a-chip inhabited by microbial flora that experiences intestinal peristalsis-like motions and flow
Hyun Jung Kim, Dongeun Huh, Geraldine Hamilton and Donald E. Ingber
DOI: 10.1039/C2LC40074J

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Top ten most accessed articles in February

This month sees the following articles in Lab on a Chip that are in the top ten most accessed:

Commercialization of microfluidic point-of-care diagnostic devices
Curtis D. Chin, Vincent Linder and Samuel K. Sia
Lab Chip, 2012, Advance Article
DOI: 10.1039/C2LC21204H

Engineers are from PDMS-land, Biologists are from Polystyrenia
Erwin Berthier, Edmond W. K. Young and David Beebe
Lab Chip, 2012, 12, 1224-1237
DOI: 10.1039/C2LC20982A

Droplet microfluidics for high-throughput biological assays
Mira T. Guo, Assaf Rotem, John A. Heyman and David A. Weitz
Lab Chip, 2012, Advance Article
DOI: 10.1039/C2LC21147E

Standards for connecting microfluidic devices?
Henne van Heeren
Lab Chip, 2012, 12, 1022-1025
DOI: 10.1039/C2LC20937C

Sorting cells by size, shape and deformability
Jason P. Beech, Stefan H. Holm, Karl Adolfsson and Jonas O. Tegenfeldt
Lab Chip, 2012, 12, 1048-1051
DOI: 10.1039/C2LC21083E

Miniaturised technologies for the development of artificial lipid bilayer systems
Michele Zagnoni
Lab Chip, 2012, 12, 1026-1039
DOI: 10.1039/C2LC20991H

Cell sorting by deterministic cell rolling
Sungyoung Choi, Jeffrey M. Karp and Rohit Karnik
Lab Chip, 2012, 12, 1427-1430
DOI: 10.1039/C2LC21225K

A microfluidic device for high throughput bacterial biofilm studies
Jeongyun Kim, Manjunath Hegde, Sun Ho Kim, Thomas K. Wood and Arul Jayaraman
Lab Chip, 2012, 12, 1157-1163
DOI: 10.1039/C2LC20800H

Gene-Z: a device for point of care genetic testing using a smartphone
Robert D. Stedtfeld, Dieter M. Tourlousse, Gregoire Seyrig, Tiffany M. Stedtfeld, Maggie Kronlein, Scott Price, Farhan Ahmad, Erdogan Gulari, James M. Tiedje and Syed A. Hashsham
Lab Chip, 2012, 12, 1454-1462
DOI: 10.1039/C2LC21226A

A completely in vitro ultrahigh-throughput droplet-based microfluidic screening system for protein engineering and directed evolution
Ali Fallah-Araghi, Jean-Christophe Baret, Michael Ryckelynck and Andrew D. Griffiths
Lab Chip, 2012, 12, 882-891
DOI: 10.1039/C2LC21035E

Why not take a look at the articles today and blog your thoughts and comments below.

Fancy submitting an article to Lab on a Chip? Then why not submit to us today or alternatively email us your suggestions.

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LOC articles on drug screening and a ‘nanoslinky’ in the press

C1LC20709A graphical abstractSiva Vanapalli and colleagues at Texas Tech University have developed a one-step passive strategy to create concentration gradients in static droplet arrays. Their recent Lab on a Chip article has been reported in PhysOrg.com, which discusses the implications of the technology for the future of drug testing.

Read the PhysOrg.com article here, or read the Lab on a Chip paper:

Microfluidic static droplet arrays with tuneable gradients in material composition
Meng Sun, Swastika S. Bithi and Siva A. Vanapalli
DOI: 10.1039/C1LC20709A

C2LC21152A graphical abstractSamuel Stavis and colleagues at the National Institute of Standards and Technology (NIST) have developed a nanoscale fluidic channel shaped like a staircase, which can be used to control the direction of drift of a DNA molecule through a fluid. An article in Nanotechnology Now discusses how this ‘nanoslinky’ presents new possibilities for manipulating and measuring DNA.

Read the article in Nanotechnology Now, or read the Lab on a Chip paper:

DNA molecules descending a nanofluidic staircase by entropophoresis
Samuel M. Stavis, Jon Geist, Michael Gaitan, Laurie E. Locascio and Elizabeth A. Strychalski
DOI: 10.1039/C2LC21152A

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