The small size and lipid bilayer structure of liposomes make them an excellent carrier for use in drug delivery and gene therapy. Determining the chemical and physical properties of a liposome is key to evaluating its potential for different applications.
A range of techniques have been used to measure factors such as size, surface charge and encapsulation efficiency of these spherical structures. In this minireview, Xiaomei Yan and colleagues at Xiamen University, China, explore recent advances in techniques for single-liposome characterization. They evaluate the latest adaptations of traditional microscopy techniques and investigate the advantages and limitations of using flow cytometry for characterisation. Recently developed commercial techniques such as scanning ion occlusion sensing (SIOS) and nanoparticle tracking analysis (NTA) also offer more advanced methods of single-liposome analysis.
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Analytical techniques for single-liposome characterization
Chaoxiang Chen, Shaobin Zhu, Tianxun Huang, Shuo Wanga and Xiaomei Yan
Anal. Methods, 2013,5, 2150-2157