Archive for February, 2013

Recognising coeliac disease

An immunosensor that recognises the specific immune response to gluten, generated by coeliac disease sufferers, has been developed by scientists in Spain and Portugal.

Coeliac disease is a chronic intolerance to gluten – a protein found in wheat, barley, rye and oats – that leads to inflammation of the small intestine. This can cause malnutrition and diarrhoea in sufferers.

To read the full article please visit Chemistry World.

An electrochemical deamidated gliadin antibody immunosensor for celiac disease clinical diagnosis
Marta M. P. S. Neves ,  María Begoña González-García ,  Henri P. A. Nouws and Agustín Costa-García
Analyst, 2013, Advance Article
DOI: 10.1039/C3AN36728B

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Analyst paper highly commended in GSK 3Rs prize

The 3Rs prize has been running since 2004, sponsored by GSK it is the annual prize of the National Centre for the Replacement, Refinement and Reduction of Animals in Research. It aims to highlight the best original contribution to science that benefits the 3Rs globally (to replace, reduce and refine the use of animals in research).

High-throughput 3D spheroid culture and drug testing using a 384 hanging drop array

The paper from Shuichi Takayama, University of Michigan, USA, has been highly commended by the judges in this years 3Rs prize:

In his paper he describes a 3D cell culture used to test anti-cancer drugs, which was shown to be more representative of clinical responses than standard 2D ‘flat’ cell cultures. This new testing method has the potential to replace and reduce the use of animals in pharmaceutical testing.

Click on the link below to read this highly commended paper. It will be free to read until March 15th.

High-throughput 3D spheroid culture and drug testing using a 384 hanging drop array
Yi-Chung Tung, Amy Y. Hsiao, Steven G. Allen, Yu-suke Torisawa, Mitchell Ho and Shuichi Takayama
Analyst, 2011, 136, 473-478
DOI: 10.1039/C0AN00609B

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A new switchable sensor for phosphate in water

Schematic interaction between Ce3+ and phosphate

Guang-Li Wang and co-workers, from the Jiangnan University, China, have developed an assay to detect the level of phosphate in water. 

Ce3+ ions are used to aggregate citrate-capped silver nanoparticles with cysteine-capped quantum dots, thereby causing efficient quenching of the fluorescence signal.  However, when phosphate is introduced to the system it interacts with the Ce3+ ions causing the quenched aggregates to redisperse and switching the fluorescent signal from “off” to “on”.  This work has potential applications for the environmental analysis of groundwater.

To read more about this work, please access this Analyst HOT article below. It will be free to read until March 8th.

Novel switchable sensor for phosphate based on the distance-dependant fluorescence coupling of cysteine-capped cadmium sulfide quantum dots and silver nanoparticles
Guang-Li Wang ,  Huan-Jun Jiao ,  Xiao-Ying Zhu ,  Yu-Ming Dong and Zai-Jun Li
Analyst, 2013, Advance Article
DOI: 10.1039/C3AN36878E

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Correlated Imaging for Chemical Analysis

Schematic picture of a hybrid instrument correlating different spectral imaging modes

Scientists use chemical imaging to address a variety of issues visualising the arrangement of atoms, the content of single cells or the colourless gases released into Earth’s atmosphere by cities of kilometre scale. Many analytical techniques have been developed to date and researchers are now interested in combining the different imaging modalities to increase and improve the information gathered from imaging experiments.

In this new Critical Review just published in Analyst Paul Bohn and his group at the University of Notre Dame, USA, explore all the latest imaging techniques and introduce the new area of Raman imaging and mass spectrometry in the field of correlated imaging.

To know more, please access the full article below. It will be free to read until March 8th.

Correlated imaging – a grand challenge in chemical analysis
Rachel Masyuko ,  Eric J. Lanni ,  Jonathan V. Sweedler and Paul W. Bohn
Analyst, 2013, Advance Article
DOI: 10.1039/C3AN36416J

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Raman spectroscopy for bedside cancer diagnosis

Currently the only way to identify whether a suspicious lump is something to be concerned about is to have it removed surgically and then examined by histological review. But a new diagnostic technique based around Raman spectroscopy could provide a faster diagnosis without the need for such invasive procedures.

Raman Spectroscopy has been used previously to distinguish benign and metastatic axillary lymph nodes (in the breast) and mediastinal nodes (in the oesophagus). Now Nicholas Stone and co-workers at the University of Exeter, UK have now applied the technique to distinguish between different cancerous conditions of lymph nodes in the head and neck.

Doctors coudl soon be using Raman spectroscopy to peform 'optical biopsies' of tumours © Shutterstock

 To read the full article please visit Chemistry World.

Discrimination between benign, primary and secondary malignancies in lymph nodes from the head and neck utilising Raman spectroscopy and multivariate analysis
Gavin Rhys Lloyd, Linda E. Orr, Jonathan Christie-Brown, Keith McCarthy, Simon Rose, Michael Thomas and Nicholas Stone
Analyst, 2013, Advance Article
DOI: 10.1039/C2AN36579K

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Showcase your own research at the Analytical Research Forum 2013 (ARF13): extended deadline

The deadline for submitting oral abstracts has been extended to 4 March 2013

This is the premier Analytical Science meeting of the RSC designed primarily for early-career analytical scientists (including PhD students and postdoctoral fellows) to present their latest results in the context of the wider analytical science community.

Oral and poster contributions from students and young researchers are welcome. Prizes for the best contributions will be awarded.

An added feature this year will be a tour of the GSK site in Stevenage, which is where the lectures and poster presentations will take place.

Don’t miss the opportunity! Submit your abstract now!

Check out the event website to find out more – http://rsc.li/arf13

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Analyst Issue 6 NOW online

The new issue of Analyst is now available online. Take a look at our wonderful covers and discover all about the latest research behind them.

Our outside front cover shows the work of Igor Lednev and his group from the State University of New York, USA. The researchers analyse biological specimens using surface- and tip-enhanced Raman spectroscopy (SERS and TERS) and investigate the lack amide I band, commonly used as a marker for interpretation of the secondary protein structure.

Amide I vibrational mode suppression in surface (SERS) and tip (TERS) enhanced Raman spectra of protein specimens
Dmitry Kurouski ,  Thomas Postiglione ,  Tanja Deckert-Gaudig ,  Volker Deckert and Igor K. Lednev
Analyst, 2013,138, 1665-1673
DOI: 10.1039/C2AN36478F

Our interesting inside front cover showcases work from Luigi Sanguigno from the Italian Institute of Technology, who describes how fluorescence correlation spectroscopy (FCS) can be misleading in 2D systems. This study highlights the alignment issues and the effects of thermal fluctuations related to FCS in 2D systems and proposes elimination of systematic errors due to the misalignments of membranes.

Accounting for misalignments and thermal fluctuations in fluorescence correlation spectroscopy experiments on membranes
Luigi Sanguigno ,  Chiara Cosenza ,  Filippo Causa and Paolo Antonio Netti
Analyst, 2013,138, 1674-1681
DOI: 10.1039/C2AN36681A

The outside back cover of this issue introduces negative mode probe electrospray ionization mass spectrometry (PESI-MS) as a tool for biomolecular analysis and cancer dignostics. Mridul Kanti Mandal and colleagues from the University of Yamanashi, Japan, analysed several combinations of solvents and probes to optmise the conditions of ionization for biomolecules such as peptides, proteins and lipids.

Biomolecular analysis and cancer diagnostics by negative mode probe electrospray ionization
Mridul Kanti Mandal ,  Subhrakanti Saha ,  Kentaro Yoshimura ,  Yasuo Shida ,  Sen Takeda ,  Hiroshi Nonami and Kenzo Hiraoka
Analyst, 2013,138, 1682-1688
DOI: 10.1039/C3AN36554A

In addition, along with our new covers we also have some selected HOT articles for you to enjoy. These papers will be free to read for the next 10 days.

Take a look at Issue 6 now!

A mixed alkanethiol based immunosensor for surface plasmon field-enhanced fluorescence spectroscopy in serum
Andreas Scholten ,  Bernhard Menges ,  Martin Juebner ,  Markus A. Rothschild and Katja Bender
Analyst, 2013,138, 1705-1712
DOI: 10.1039/C3AN35657D

A Hg2+-mediated label-free fluorescent sensing strategy based on G-quadruplex formation for selective detection of glutathione and cysteine
Jingjin Zhao ,  Chunfei Chen ,  Liangliang Zhang ,  Jianhui Jiang ,  Guoli Shen and Ruqin Yu
Analyst, 2013,138, 1713-1718
DOI: 10.1039/C3AN36657J

Iodine excitation in a dielectric barrier discharge micro-plasma and its determination by optical emission spectrometry
Yong-Liang Yu ,  Shuai Dou ,  Ming-Li Chen and Jian-Hua Wang
Analyst, 2013,138, 1719-1725
DOI: 10.1039/C2AN36780G

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DNA Detection Using Novel Fiber-Optic Biosensing Strategy

DNA Detection Using Fiber Optic Biosensor

Overview of DNA Detection Using Fiber Optic Biosensor

Ming-jie Yin and others from The Hong Kong Polytechnic University in China have made the latest stride towards development of label-free fiber-optic biosensors for DNA detection.

Their new design involves fabrication of a DNA sensor as part of a thin sensing film on the surface of a thin-core fiber modal interferometer (TCFMI) to measure differences in refractive-index upon detection of target DNA sequences. The research team makes use of a layer-by-layer self-assembly approach, which enables controlled tunability in preparation of the sensing film.

Unlike many other reported fiber-optic biosensing methods, this current strategy confers the advantages of high detection sensitivity, lower cost and ease in preparation.

Read more about the latest discovery by accessing the link below. This article will be free to read until March 6th.

Label-free, disposable fiber-optic biosensors for DNA hybridization detection
Ming-jie Yin ,  Chuang Wu ,  Li-yang Shao ,  Wing Kin Edward Chan ,  A. Ping Zhang ,  Chao Lu and Hwa-yaw Tam
Analyst, 2013, Advance Article
DOI: 10.1039/C3AN36791F

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Rayleigh, Ramsay, Rutherford and Raman

In his recently published Editorial, Robin Clark talks about the key contributions of the four great Nobel Laureates – Lord Rayleigh, Sir William Ramsay, Lord Rutherford and Sir Chandrasekhara Raman – to the understanding of light scattering, to the identification and classification of the rare gases, and to the discovery of the Raman effect.

Rayleigh, Ramsay, Rutherford and Raman

Rayleigh, Ramsay, Rutherford and Raman – their connections with, and contributions to, the discovery of the Raman effect
Robin J. H. Clark
Analyst, 2013, 138, 729-734
DOI: 10.1039/C2AN90124B

To commemorate this wonderful editorial, we have gathered together a collection of papers from the last few year on Raman spectroscopy that have been published in Analyst. These papers will be free to read until March 22nd. Enjoy!

SERS-based sandwich immunoassay using antibody coated magnetic nanoparticles for Escherichia coli enumeration
Burcu Guven, Nese Basaran-Akgul, Erhan Temur, Ugur Tamer and İsmail Hakkı Boyacı
Analyst, 2011, 136, 740-748
DOI: 10.1039/C0AN00473A

Evaluation of tip-enhanced Raman spectroscopy for characterizing different virus strains
Peter Hermann, Antje Hermelink, Veronika Lausch, Gudrun Holland, Lars Möller, Norbert Bannert and Dieter Naumann
Analyst, 2011, 136, 1148-1152
DOI: 10.1039/C0AN00531B

Surface enhanced optical spectroscopies for bioanalysis
Iain A. Larmour and Duncan Graham
Analyst, 2011, 136, 3831-3853
DOI: 10.1039/C1AN15452D

Recent advancements in optical DNA biosensors: Exploiting the plasmonic effects of metal nanoparticles
Hsin-I Peng and Benjamin L. Miller
Analyst, 2011, 136, 436-447
DOI: 10.1039/C0AN00636J

Non-invasive analysis of turbid samples using deep Raman spectroscopy
Kevin Buckley and Pavel Matousek
Analyst, 2011, 136, 3039-3050
DOI: 10.1039/C0AN00723D

Non-invasive analysis of turbid samples using deep Raman spectroscopy

Buckley & Matousek, Analyst, 2011, 136, 3039

Subcellular localization of early biochemical transformations in cancer-activated fibroblasts using infrared spectroscopic imaging
Sarah E. Holton, Michael J. Walsh and Rohit Bhargava
Analyst, 2011, 136, 2953-2958
DOI: 10.1039/C1AN15112F

Poor quality drugs: grand challenges in high throughput detection, countrywide sampling, and forensics in developing countries
Facundo M. Fernandez, Dana Hostetler, Kristen Powell, Harparkash Kaur, Michael D. Green, Dallas C. Mildenhall and Paul N. Newton
Analyst, 2011, 136, 3073-3082
DOI: 10.1039/C0AN00627K

Rapid, sensitive DNT vapor detection with UV-assisted photo-chemically synthesized gold nanoparticle SERS substrates
Maung Kyaw Khaing Oo, Chia-Fang Chang, Yuze Sun and Xudong Fan
Analyst, 2011, 136, 2811-2817
DOI: 10.1039/C1AN15110J

Surface enhanced Raman scattering for multiplexed detection
Jennifer A. Dougan and Karen Faulds
Analyst, 2012, 137, 545-554
DOI: 10.1039/C2AN15979A

Direct SERS detection of contaminants in a complex mixture: rapid, single step screening for melamine in liquid infant formula
Jordan F. Betz, Yi Cheng and Gary W. Rubloff
Analyst, 2012, 137, 826-828
DOI: 10.1039/C2AN15846A

A quantitative solid-state Raman spectroscopic method for control of fungicides
Bojidarka Ivanova and Michael Spiteller
Analyst, 2012, 137, 3355-3364
DOI: 10.1039/C2AN35174A

Extracting biological information with computational analysis of Fourier-transform infrared (FTIR) biospectroscopy datasets: current practices to future perspectives
Júlio Trevisan, Plamen P. Angelov, Paul L. Carmichael, Andrew D. Scott and Francis L. Martin
Analyst, 2012, 137, 3202-3215
DOI: 10.1039/C2AN16300D

Differentiating intrinsic SERS spectra from a mixture by sampling induced composition gradient and independent component analysis
Justin L. Abell, Joonsang Lee, Qun Zhao, Harold Szu and Yiping Zhao
Analyst, 2012, 137, 73-76
DOI: 10.1039/C1AN15623C

Surface enhanced Raman spectroscopy for microfluidic pillar arrayed separation chips
Lisa C. Taylor, Teresa B. Kirchner, Nickolay V. Lavrik and Michael J. Sepaniak
Analyst, 2012, 137, 1005-1012
DOI: 10.1039/C2AN16239C

Total internal reflection Raman spectroscopy
David A. Woods and Colin D. Bain
Analyst, 2012, 137, 35-48
DOI: 10.1039/C1AN15722A

Understanding the molecular information contained in principal component analysis of vibrational spectra of biological systems
F. Bonnier and H. J. Byrne
Analyst, 2012, 137, 322-332
DOI: 10.1039/C1AN15821J

2p or not 2p: tuppence-based SERS for the detection of illicit materials

Goodacre et al., Analyst, 2013, 138, 118

Streptococcus suis II immunoassay based on thorny gold nanoparticles and surface enhanced Raman scattering

Kun Chen, Heyou Han and Zhihui Luo
Analyst, 2012, 137, 1259-1264
DOI: 10.1039/C2AN15997J

2p or not 2p: tuppence-based SERS for the detection of illicit materials
Samuel Mabbott, Alex Eckmann, Cinzia Casiraghi and Royston Goodacre
Analyst, 2013, 138, 118-122
DOI: 10.1039/C2AN35974J

Toward development of a surface-enhanced Raman scattering (SERS)-based cancer diagnostic immunoassay panel
Jennifer H. Granger, Michael C. Granger, Matthew A. Firpo, Sean J. Mulvihill and Marc D. Porter
Analyst, 2013, 138, 410-416
DOI: 10.1039/C2AN36128K

3D confocal Raman imaging of endothelial cells and vascular wall: perspectives in analytical spectroscopy of biomedical research
Katarzyna Majzner, Agnieszka Kaczor, Neli Kachamakova-Trojanowska, Andrzej Fedorowicz, Stefan Chlopicki and Malgorzata Baranska
Analyst, 2013, 138, 603-610
DOI: 10.1039/C2AN36222H

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HOT Articles in Analyst!

In the latest issue of Analyst we have some wonderful HOT articles for you to read. They cover a wide variety of topics including iodine determination by optical emission spectrometry, detection of trinitrotoluene in seawater, and immunosensors for surface plasmon field-enhanced fluorescence spectroscopy in serum.

If you want to know more about the latest discoveries published in Analyst, take a look at the papers below, they will be free to read until February 28th.

Iodine excitation in a dielectric barrier discharge micro-plasma and its determination by optical emission spectrometry
Yong-Liang Yu ,  Shuai Dou ,  Ming-Li Chen and Jian-Hua Wang
Analyst, 2013, Advance Article
DOI: 10.1039/C2AN36780G

Graphenes prepared from multi-walled carbon nanotubes and stacked graphene nanofibers for detection of 2,4,6-trinitrotoluene (TNT) in seawater
Shu Min Tan ,  Chun Kiang Chua and Martin Pumera
Analyst, 2013, Advance Article
DOI: 10.1039/C3AN00089C

Graphene nanopfibers for detection of trinitrotoluene

A mixed alkanethiol based immunosensor for surface plasmon field-enhanced fluorescence spectroscopy in serum
Andreas Scholten ,  Bernhard Menges ,  Martin Juebner ,  Markus A. Rothschild and Katja Bender
Analyst, 2013, Advance Article
DOI: 10.1039/C3AN35657D

Facile formation of folic acid-modified dendrimer-stabilized gold–silver alloy nanoparticles for potential cellular computed tomography imaging applications
Hui Liu ,  Mingwu Shen ,  Jinglong Zhao ,  Jingyi Zhu ,  Tingting Xiao ,  Xueyan Cao ,  Guixiang Zhang and Xiangyang Shi
Analyst, 2013, Advance Article
DOI: 10.1039/C3AN36649A

In-process phase growth measurement technique in the fabrication of monolithic porous layer open tubular (monoPLOT) columns using capacitively coupled contactless conductivity
David A. Collins ,  Ekaterina P. Nesterenko ,  Dermot Brabazon and Brett Paull
Analyst, 2013, Advance Article
DOI: 10.1039/C3AN00133D

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