In their recent paper Ryan Bailey and colleagues at the University of Illinois at Urbana-Champaign address the challenge of creating a biomarker assay capable of spanning a clinically relevant dynamic range. The ability to accurately detect protein biomarkers over a wide dynamic range is extremely important to determine the stage a disease is at, as well as monitoring the effects of any treatments.
They have developed a a silicon photonic microring resonator-based platform that can quantify the cardiovascular risk biomarker C-reactive protein over a dynamic range of six orders of magnitude. The 3 step assay increases the dynamic range beyond that possible for a single-step assay and also reduces false positive results.
Download the article for the details – the article is currently free to access:
Sensitive on-chip detection of a protein biomarker in human serum and plasma over an extended dynamic range using silicon photonic microring resonators and sub-micron beads
Matthew S. Luchansky, Adam L. Washburn, Melinda S. McClellan and Ryan C. Bailey
Lab Chip, 2011, 11, 2042-2044
DOI: 10.1039/C1LC20231F
