Pietro Ferraro and colleagues from three Italian research institutes have used digital holography to image objects in turbid flowing media in microfluidic devices. Although many current techniques are able to provide in situ images of liquids flowing in microfluidic devices, they are limited to cases of clear liquids with relatively stable flows. Due to the Doppler effect of flowing colloidal particles the researchers were able to reconstruct digital holographic images, and demonstrated this with milk:
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Microscopy imaging and quantitative phase contrast mapping in turbid microfluidic channels by digital holography
Melania Paturzo, Andrea Finizio, Pasquale Memmolo, Roberto Puglisi, Donatella Balduzzi, Andrea Galli and Pietro Ferraro
DOI: 10.1039/C2LC40114B