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Drinking tea could help you shift those festive season pounds

A team from The Pennsylvania State University have shown that one of the major polyphenols in green tea, (–)-Epigallocatechin-3-gallate (EGCG), increases the expression of genes related to fat oxidation.  EGCG has been show to prevent the development of obesity in rodent models and may also modulate body weight in humans.  Mechanisms accounting for this effect that have been demonstrated include a decrease of fat absorption in the small intestine and modulation of genes related to lipid metabolism in the liver, white adipose tissue and skeletal muscle; although in the latter tissue, comparatively little is known.

(−)-Epigallocatechin-3-gallate increases the expression of genes related to fat oxidation in the skeletal muscle of high fat-fed mice Joshua D. Lambert and co-workers investigated the expression of several genes related to lipid oxidation in the skeletal muscle of high fat-fed mice.  They also compared these changes to observed effects on physiological markers of obesity, type II diabetes and obesity-related fatty liver disease (ORLFD).   

Results showed that high fat-fed mice treated with EGCG had reduced body weight gain and final body weight compared to high fat-fed controls.  EGCG treatment also decreased fasting blood glucose, plasma insulin, insulin resistance and markers of ORFLD.  The expression of mRNA from genes relating to mitochondrial fatty acid oxidation was increased as was the levels of fat in the excretion.  Taken together, these results suggest that EGCG modulates body weight gain by modulating both lipid metabolism and fat absorption.

green teaSo, it may be that drinking green tea could help with those new years resolutions to shed a few pounds; however, you’ll have to drink a lot as the levels used in this study correspond to a human consumption of approximately 10 cups a day!

Interested in knowing more? Read the full text here.

(−)-Epigallocatechin-3-gallate increases the expression of genes related to fat oxidation in the skeletal muscle of high fat-fed mice
Sudathip Sae-tan, Kimberly A. Grove, Mary J. Kennett and Joshua D. Lambert

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Can herbal tea provide the cure for Alzheimer’s?

A team from the University of Lisbon in Portugal has demonstrated, in vivo, the effects of a herbal tea (Lamiaceae) which may have beneficial effects on Alzheimer’s Disease.

Function of Plectranthus barbatus herbal tea as neuronal acetylcholinesterase inhibitor Leaves of Plectranthus barbatus (Lamiaceae) are used to make herbal teas and as a traditional remedy for a wide range of diseases, recently they have been shown, in vitro, to possess anti-oxidant and anti-acetylcholinesterase activity.  Acetylcholinesterase (AChE) inhibition is the most effective pharmacotherapy for the symptomatic treatment of Alzheimer’s disease.  The active component responsible from Lamiaceae has been identified as rosmarinic acid, however, effects of this compound in the body are dependent on its metabolism.  It is these metabolites and the places they reach that will exert a physiological effect.  In this study Serralheiro and colleagues set out to determine, in vivo, if Lamiaceae herbal tea and pure rosmarinic acid could pass the digestive tract and keep some of their functions, particularly in the brain given the potential Alzheimer’s benefits.

Lamiaceae herbal tea and pure rosmarinic acid were administered to rats intragastrically and intraperitoneally.  The resulting metabolites in the plasma and brain were studied as was brain AChE activity.  Upon intragastric administration of tea, only traces of metabolites were found in plasma and none in the brain.  However, a decrease in brain AChE activity of about 10% was detected.  When pure rosmarinic acid was administered intragastrically it was detected in the plasma.  Upon intraperitoneal administration of tea all metabolites were detected in plasma and rosmarinic acid detected in the brain; resulting in a decrease in brain AChE of about three times that of intragastric administration.

Taken together the results suggest that the rosmarinic acid present in herbal teas may cross the intestinal barrier as well as the blood brain barrier.  It has also been shown that in the brain rosmarinic acid inhibits AChE activity.

Read the full text for free here!

Function of Plectranthus barbatus herbal tea as neuronal acetylcholinesterase inhibitor 
Pedro L. V. Falé, Paulo J. Amorim Madeira, M. Helena Florêncio, Lia Ascensão and Maria Luísa M. Serralheiro
Food Funct., 2011, Advance Article
DOI: 10.1039/C0FO00070A, Paper

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Pleasures of Chocolate

Alejandro MarangoniFood & Function Editorial Board member, Alejandro Marangoni recently spoke at the Unilever-RSC International Symposium on Functional Materials.  This series of three one day meetings was held from the 8th-11th November in Beijing, Hangzhou and Shanghai. Alejandro’s first two talks focussed on nanoscale structures in fats and his final one on nanostructuring liquid oils into functional fats.

ChocolateSensory attributes of fat structure materials such as butter and chocolate are mainly related to the structure and properties of a network of triacylglycerols, polycrystals and crystal aggregates present.  Alejandro described the nanoscale structure and intercrystalline interactions in chocolate which explain its pleasures.  His group have discovered that the general structure of a fat crystalline network starts with the association of nanoplatelets at the lowest structural level.  These nanoplatelets interact and aggregate via van der Waals’s forces into larger fractal structures to form a three-dimensional matrix.  These new insights are contributing to the knowledge of the nature of fat crystal networks and the relationship between these structures to the functional properties of edible fats.

butterAlejandro’s last talk reviewed novel strategies for nanostructuring liquid oils into functional fats.  This is an area of increasing interest due to public concerns over excessive saturated and trans fat intake from manufactured food products.  Alejandro described various strategies: using surfactant-like small molecules, phytosterols and ceramides as organogelators, structuring liquid oils by microencapsulation within multilamellar vesicles and the use of high-molecular weight polymers such as ethylcellulose to gel oil in the absence of water.

Do you work in this field? Submit your work to Food & Function today!

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Emulsion-based delivery systems: reviewing digestion models and interfacial design

Issues 1 and 2 of Food & Function are now here, and the vision of pulling together high impact chemical and physical research and linking it to human health and nutrition is starting to be fulfilled. 

This week on the blog we are highlighting reviews published from the physics community.

Review of in vitro digestion models for rapid screening of emulsion-based systems In Issue 1 ‘Review of in vitro digestion models for rapid screening of emulsion-based systems’ by David Julian McClements and Yan Li looks at the current status of in vitro digestion models for simulating lipid digestion.  Emulsion-based delivery systems are being developed to encapsulate, protect, and release non-polar lipids, vitamins, nutraceuticals and drugs.  There is, therefore, of increasing interest in the food and pharmaceutical industries to understand and control the digestion of these emulsified lipids.  To do this, in vitro digestion models which simulate the human gastrointestinal tract are needed to test the efficacy of different approaches for controlling lipid digestion.

 

As a continuation, Issue two contains a review which covers the physico-chemical changes occurring in emulsion based delivery systems during gastric and small intestine digestion.  In ‘Interfacial design of protein-stabilized emulsions for optimal delivery of nutrients’ by Amir Malaki Nik, Amanda J. Wright and Milena Corredig protein-stabilised oil-in-water emulsions are focused on.  Proteins are often used as ingredients in food emulsions, as their amphiphilic structures provide electrostatic and steric stabilisation. A better understanding of how to tailor the composition of oil droplet surfaces in food emulsions will aid in optimizing lipid digestion and, as a result, delivery of lipophilic nutrients. Interfacial design of protein-stabilized emulsions for optimal delivery of nutrients

Interested in reading more? Follow the links below:

Review of in vitro digestion models for rapid screening of emulsion-based systems

Interfacial design of protein-stabilized emulsions for optimal delivery of nutrients

You may also want to submit a review or an article linking the physics of food with health and nutrition.

Manuscripts can be submitted online here 

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Liquorice compounds show dual anti-cancer and anti-inflammatory properties

Graphical abstract: Inhibitory effects of 1,3-bis-(2-substituted-phenyl)-propane-1,3-dione, β-diketone structural analogues of curcumin, on chemical-induced tumor promotion and inflammation in mouse skin‘Dibenzoylmethanes (DBMs), isolated from liquorice, have excellent anti-inflammatory and anti-cancer effects,’ claim scientists in Taiwan and the US.

DBMs are β-diketone structural analogues of curcumin and have an aspirin-like skeleton. Curcumin and aspirin are known to possess anti-inflammatory and chemopreventive effects through suppression of COX-2 gene expression.  Due to the structural similarities between DBM and curcumin and aspirin, Chuan-Chuan Lin and co-workers tested the anti-inflammatory and anti-cancer effects of DBMs. They found DBMs to have the potential to substitute for aspirin in therapeutic anti-inflammation treatment. In addition, they also noted the DBMs activities as an anticancer agent.

The team from the China University of Science and Technology and the State University of New Jersey in the US believes that DMBs acts by inhibiting the COX-2 enzyme. It is known that expression of COX-2 is associated with chronic inflammation and epithelial carcinogenesis. The team used the tumour promoting agent 2-O-Tetradecanoylphorbol-13-acetate (TPA), which induces COX-2 expression causing tumours and ear edema in the skin of mice. Lin et al. found that DBMs inhibited TPA-induced skin tumours significantly and that some of the DMB compounds possessed superior anti-inflammatory properties than aspirin. 

Read more about this paper here:
Inhibitory effects of 1,3-bis-(2-substituted-phenyl)-propane-1,3-dione, β-diketone structural analogues of curcumin, on chemical-induced tumor promotion and inflammation in mouse skin
Chuan-Chuan Lin, Yue Liu, Chi-Tang Ho and Mou-Tuan Huang
Food Funct., 2011, Advance Article
DOI: 10.1039/C0FO00098A, Paper

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Broccoli’s cancer protection is activated by bacteria in the lower gut

Bacteria in the lower gut are responsible for breaking down of the major cancer fighting chemical in broccoli to release its bioactive form, claim Elizabeth Jeffery and colleagues at the University of Illinois.

Cruciferous vegetables such as broccoli have been shown to reduce cancer risks. Of all the components present in crucifers such as antioxidants and flavonoids, it is believed that a molecule called glucoraphanin (GRP) plays a major role explaining their anticancer properties.  GRP is actually a precursor to the bioactive isothocyanate sulforaphane (SF).  It is hydrolysed to SF by an endogenous enzyme in broccoli called myrosinase.  Upon chewing, the myrosinase gains access to the GRP and catalyses hydrolysis within the gastrointestinal tract, however, in cooked broccoli myrosinase is inactivated yet low levels of SF metabolites appear in urine following ingestion, suggesting hydrolysis has, somehow, occurred.

Glucoraphanin hydrolysis by microbiota in the rat cecum results in sulforaphane absorption

Evidence exists that gut bacteria are responsible for GRP hydrolysis but this study is the first to report direct evidence of hydrolysis of a GRP to SF in the lower gut. Jeffery and colleagues investigated, in rats, the hydrolysis by gut bacteria and absorption across the cecum (lower intestine) of GRP from broccoli.  Simulated digestion in vitro confirmed that GRP is not destroyed by digestive enzymes therefore reaches the cecum intact.  Introduction of GRP directly to the cecum resulted in the appearance of SF and SF metabolites in the blood travelling away from the abdomen after 2 hours; in contrast, direct introduction of SF resulted in detection of SF and SF metabolites after only 15 minutes. 

These results show for the first time that SF can be absorbed by the cecum, they also indicate that GRP is broken down to SF in the cecum and then absorbed into the bloodstream.  Finally, an ex vivo study showed that GRP was hydrolysed by the rat’s gut bacteria, however, the hydrolysis product was not SF; reasons for the difference in GRP breakdown in and ex vivo are discussed.

Interested in knowing more? Read the full article here:

Glucoraphanin hydrolysis by microbiota in the rat cecum results in sulforaphane absorption
Ren-Hau Lai, Michael Miller and Elizabeth Jeffery
Food Funct., 2010, DOI:10.1039/C0FO00110D

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Should superfoods be consumed in moderation?

Ronald L. Prior and co-workers in the USA calim that inclusion of cranberry in the diet is effective in modulating some aspects of the metabolic parameters associated with metabolic syndrome.  However, a high dose of cranberries does not neccessarily result in a metabolic response.

Metabolic syndrome refers to the clustering of cardiometabolic risk factors, although it was believed initially to be associated with increased risk of cardiovascular disease; metabolic syndrome has a stronger association with type 2 diabetes.  A characteristic of type 2 diabetes and metabolic syndrome is insulin resistance, a condition where insulin becomes less effective at reducing glucose levels in the body.  Effects of dietary consumption of cranberry powder on metabolic parameters in growing rats fed high fructose diets

Recently, cinnamon has been shown to reduce plasma glucose levels in diabetic patients and a class of phenolic phytochemicals called A-type procyanidins are responsible for this.  A-type procyanidins are only found in a limited number of foods and other than cinnamon, cranberry has the highest concentration.

The team from the U.S. Department of Agriculture and the University of Arkansas set out to determine if phytochemicals in cranberry were effective in normalising selected metabolic parameters associated with metabolic syndrome in high fructose (HF) fed rats.  Rats were fed on low, medium or high levels of cranberry powder (CP).  Fasting plasma glucose and triglycerides were higher in HF fed rats than control rats and were reduced by feeding CP; similarly, oral glucose tolerance test responses were improved and similar to control animals when fed low or medium levels of CP.  Insulin resistance and β-cell function were reduced by CP with medium levels being most effective, furthermore, kidney weight was higher in the HF fed group but feeding with CP decreased kidney weight to normal levels.  More importantly, Prior et al. highlights the importance of dose-response studies and that more is not always better.

Interested in knowing more? Read the full article here:

Effects of dietary consumption of cranberry powder on metabolic parameters in growing rats fed high fructose diets
Ramesh C. Khanal, Theodore J. Rogers, Samuel E. Wilkes, Luke R. Howard and Ronald L. Prior
Food & Funct., 2010, Advance Article
DOI: 10.1039/C0FO00089B , Paper

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Interview: We are what we eat

Gary Williamson, Editor in Chief of Food & Function, talks to Kathleen Too about low dose long term nutrition studies, EU regulators and challenges faced by scientists in food industry and academia.

Gary Williamson Gary Williamson is Professor of Functional Food at the University of Leeds, UK where his research interests lie in nutritional and food biochemistry. Before joining the university in 2007, he was head of the nutrient bioavailability group at the Nestle Research Center in Lausanne, Switzerland. He is also editor-in-chief for Food & Function, a new journal from RSC Publishing.
 

How did you become interested in food science research?

After completing my PhD and postdoc in biochemistry, I went to work for the Institute of Food Research in Norwich and gradually became more and more interested in plants and secondary metabolites and their biological activity. Eventually, this led to more studies on functional foods and their biochemical aspects. I have to say also, that I really enjoy good food. So food research has always been one of my main personal interests.

Your research focuses on functional foods and nutraceuticals, can you explain what these terms mean?

Functional foods are foods that have an activity above and beyond basic nutrition. Nutraceuticals tend to cover food extracts and supplements with a biological activity or with a proposed biological activity.

You’ve worked in both industry and academia, what are the different challenges facing scientists in these areas?

In industry, one problem is that there is a perceived bias in your research. If a company is doing a study on a particular product, then it is seen by the outside world that the company will do all it can for this product to succeed. But, actually, when I was at Nestlé, I can safely say that a lot more evidence was required to prove the benefit of a particular food to convince the managers to believe in the product. So in industry, the scientists are a lot more critical in the initial stages of a discovery and the perceived bias from the outside world is often not correct. In industry, you have less freedom to do research but more resources are available compared to academia. Sometimes, in industry, the scientists themselves can have different goals to the company goals. In academia, the biggest challenge is the money. Always money!

What kind of research do you think food industry should be funding?

Generally, the food industry has a preference for short term studies. But these may not give them the results that they like. It would be really good if industry could fund longer term studies, lasting years rather than weeks, in human nutrition. It is important to do long term low dose studies which are more difficult to conduct and to obtain measurable effects. Most scientists do pharmaceutical-type studies which are acute and high dose studies. The main challenge is to find new techniques that would allow us to do these long term low dose studies. People eat and drink all the time, so how do we study the effects of what they have been eating or drinking all their life? This is the main challenge.

How long does it take from the conception of an idea to its commercialisation?

What are the main barriers to overcome? It always takes too long to commercialise a product especially from the point of view of non-scientists in industry, such as managers and marketing people. It can take anywhere between 2 and 20 years. The main issue is not how long it takes but the different expectations of the different people on the project. That’s why marketing people have to understand that science is slow and painstaking and the scientists have to understand that marketing people cannot wait as long as they want them to wait. The other barrier is the regulators who are becoming stricter all the time. They are trying to apply the rules from the pharmaceutical industry to that of nutrition and I think that it is never going to work like that. Regulatory hurdles are becoming greater and if they are not careful, they may well strangle the science.

Why did you leave industry?

I left industry because I was keener to work on my own research and not necessarily on a specific product. I wanted to be in a university environment with PhD students and surrounded by the general ‘university expertise’. Also I reached the level I could in industry without going into more managerial roles and away from research, which I did not want to do.

What are your tips to become a successful scientist?

The bottom line is look after the people that work for you and motivate them to be at their best. The success of every project is a team effort. If you do not invest time in the people you work with, then they do not get the best out of the work and they do not get to discuss their ideas with you. My approach is to try to get them to do the best job they can and to do a first class thesis. This would benefit them for the rest of their life and also me in advancing my research.

As the editor-in-chief for the new RSC journal Food & Function, could you comment on the aims of this journal?

There is a real need for a new journal focussing on the novel aspects of food and nutrition and not just the conventional nutrition research that has been around for decades but covers some of the newer ideas of how food affects the health and how we can modify its structure to improve its health aspects.

If you weren’t a scientist, what would you do?

I am a keen photographer so maybe I would have my own photography company. Alternatively, I would like to think that I could have been a good barrister.

Also of interest

Food & Function: A new peer-reviewed journal linking the chemistry and physics of food with health and nutrition. Simply register to access all the Food & Function articles for free.

Chemistry for a healthy diet: Interview with Cesar Fraga, Associate Editor of Food & Function

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How do onions help our heart?

Hypocholesterolemic activity of onion is mediated by enhancing excretion of fecal sterols in hamstersThey may make us cry but onions are actually good for us, and researchers in China are beginning to explain why…

Lei Guan, Hau Yin Chung*, Yalun Su,
Rui Jiao, Cheng Peng and Zhen Yu Chen*
Food & Funct., 2010, Advance Article
DOI: 10.1039/C0FO00036A, Paper

 Levels of plasma total cholesterol (TC) correlate directly with the risk of coronary heart disease, one of the biggest killers worldwide.  Previous studies have shown that onion favourably modifies TC levels, but the underlying mechanism is not understood. 

Hau Yin Chung, Zhen Yu Chen and co-workers studied the effect of dietary onion powder with the protein expression of key receptors and enzymes involved in cholesterol metabolism.  Results demonstrated that onion decreased plasma TC in a dose-dependent manner, accompanied by enhanced excretion of fecal sterols.

Expression analysis demonstrated an upregulation in the expression of liver X receptor-alpha (LXRα), a protein which activates the production of cholesterol-7α-hydroxylase (CYP7A1).  CYP7A1 is a rate-limiting enzyme in the conversion of cholesterol to bile acids and is responsible for the elimination of excessive cholesterol in the liver.  This study shows that it is the upregulation of CYP7A1 which is most likely to explain the decreased plasma TC and enhanced fecal sterol excretion.

Interested in knowing more?  Read the full article here.

Simply register to access all the Food & Function articles for free.

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