A method for automatically tracking single fluorescent molecules has been described by scientists developing new techniques in single molecule microscopy (SMM).
The ability to detect and follow single fluorescent molecules provides a unique insight into the diffusion processes occurring in material science and biological systems. SMM is fraught with technical challenges, however, with low signal-to-noise-ratios and ‘blinking’ of fluorescent molecules making them difficult to track.
To overcome these issues, a new method of data analysis is described, and made available to the scientific community as open-source Matlab code by the authors.
Read this HOT PCCP article in full:
A novel method for automatic single molecule tracking of blinking molecules at low intensities
Dominik Wöll, Christoph Kölbl, Beate Stempfle and Andreas Karrenbauer
DOI: 10.1039/C3CP44693J
This article is part of the PCCP web collection on biophysics and biophysical chemistry – take a look today!
