Analyst Blog

Chemical interactions and fate of nanoprobes upon entering living cells

Take a look at our new HOT papers free for you to read until May 13^th !

Do you know what happens to nanoprobes once they enter living cells? And did you hear about  in-column molecularly imprinted solid phase extraction concentrators for capillary electrophoresis?

To know more about the latest research published in Analyst, click on the links below and enjoy the reading!

LED-induced in-column molecular imprinting for solid phase extraction/capillary electrophoresis
Xinfeng Zhang ,  Shuxia Xu ,  Yong-Ill Lee and Steven A. Soper
Analyst, 2013, Advance Article
DOI: 10.1039/C3AN00257H

New light emitting diode polymerization technology

A thin-layered chromatography plate prepared from naphthalimide-based receptor immobilized SiO2 nanoparticles as a portable chemosensor and adsorbent for Pb2+
Sunhong Park ,  Jin Hyeok Lee and Jong Hwa Jung
Analyst, 2013, Advance Article
DOI: 10.1039/C3AN00292F

Off-line reaction monitoring of the oxidation of alkenes in water using drop coating deposition Raman (DCDR) spectroscopy
Shaghayegh Abdolahzadeh ,  Nicola M. Boyle ,  Apparao Draksharapu ,  Andrew C. Dennis ,  Ronald Hage ,  Johannes W. de Boer and Wesley R. Browne
Analyst, 2013, Advance Article
DOI: 10.1039/C3AN00330B

Probing site-exclusive binding of aqueous QDs and their organelle-dependent dynamics in live cells by single molecule spectroscopy
Chaoqing Dong,a   Basudev Chowdhurya and   Joseph Irudayaraj*a  
Analyst, 2013, Advance Article
DOI: 10.1039/C3AN36906D

FTIR based classification of carcinoma regions in brain tissue

Cancer is one of the top causes of death in the world, particularly for developed countries. Regardless of the cancer type, up to 40% of all cases metastasize to the brain during disease progression. Indeed, better survival rates are possible with early and accurate cancer detection.

While histopathologic evaluation involving staining of brain tissue is the current gold standard method for diagnosis, major drawbacks include the complexity of analysis and the non-specific staining of some dyes for non-cancerous cells. Moreover, histopathological staining, and other screening methods often cannot identify the primary tumor of brain metastasis. Without knowledge of the cancer origin, determination of the optimal treatment strategy can be difficult.

Christoph Krafft and colleagues from the Institute of Photonic Technology of Jena, Germany, have developed a strategy to help identify the primary tumor by using Fourier transform infrared (FTIR) and associated software. By analysing brain metastasis tissue, the “molecular fingerprint”, or vibrational spectra characteristic of the primary tumor can be found to deduce the cancer source.

Learn more about this exciting discovery by accessing the link below. This paper will be free to read until April 29^th:

Tumor margin identification and prediction of the primary tumor from brain metastases using FTIR imaging and support vector machines
Norbert Bergner ,  Bernd F. M. Romeike ,  Rupert Reichart ,  Rolf Kalff ,  Christoph Krafft and Jürgen Popp
Analyst, 2013, Advance Article
DOI: 10.1039/C3AN00326D

Take a look at our new HOT papers free for you to discover all about!
They cover a wide range of topics including the development a ruthenium-based smart probe for selective recognition of cations using a single optical technique and the optimization of a neuraminidase assay successfully integrated into a single-step operation. To read the full papers, please access the links below.
They will be free to read until April 29th.

A stimuli-responsive “smart probe” for selective monitoring of multiple-cations via differential analyses
Anup Kumar ,  Alok K. Singh and Tarkeshwar Gupta
Analyst, 2013, Advance Article
DOI: 10.1039/C3AN00087G

A dual channel optical detector for trace water chemodosimetry and imaging of live cells
Guangwen Men ,  Guirong Zhang ,  Chunshuang Liang ,  Huiling Liu ,  Bing Yang ,  Yuyu Pan ,  Zhenyu Wang and Shimei Jiang
Analyst, 2013, Advance Article
DOI: 10.1039/C3AN36887D

Spectral cytopathology: new aspects of data collection, manipulation and confounding effects
Miloš Miljković ,  Benjamin Bird ,  Kathleen Lenau ,  Antonella I. Mazur and Max Diem
Analyst, 2013, Advance Article
DOI: 10.1039/C3AN00185G

Integration of neuraminidase inhibitor assay into a single-step operation using a combinable poly(dimethylsiloxane) capillary sensor
Tadashi Ishimoto ,  Kaede Jigawa ,  Terence G. Henares ,  Tatsuro Endo and Hideaki Hisamoto
Analyst, 2013, Advance Article
DOI: 10.1039/C3AN36785A

A high-performance waveguide-mode biosensor for detection of factor IX using PEG-based blocking agents to suppress non-specific binding and improve sensitivity
Thangavel Lakshmipriya ,  Makoto Fujimaki ,  Subash C. B. Gopinath ,  Koichi Awazu ,  Yukichi Horiguchi and Yukio Nagasaki
Analyst, 2013, Advance Article
DOI: 10.1039/C3AN00298E

Take a look at the HOT papers recently published in Analyst! They include a wide variety of topics, from the use of Raman spectroscopy for detection of cancer and malaria to the development of new analytical techniques to probe gas phase reactions and temperatures. These papers will be free to read until April 19th. Have a read now!

SERS analysis blood test for non invasive cervical cancer detection

Blood plasma surface-enhanced Raman spectroscopy for non-invasive optical detection of cervical cancer
Shangyuan Feng ,  Duo Lin ,  Juqiang Lin ,  Buhong Li ,  Zufang Huang ,  Guannan Chen ,  Wei Zhang ,  Lan Wang ,  Jianji Pan ,  Rong Chen and Haishan Zeng
Analyst, 2013, Advance Article
DOI: 10.1039/C3AN36890D

Raman spectroscopic analysis of malaria disease progression via blood and plasma samples
Alison J. Hobro ,  Aki Konishi ,  Cevayir Coban and Nicholas I. Smith
Analyst, 2013, Advance Article
DOI: 10.1039/C3AN00255A

Raman spectroscopiuc analysis of malaria disease

Radical induced disulfide bond cleavage within peptides via ultraviolet irradiation of an electrospray plume
Craig A. Stinson and Yu Xia
Analyst, 2013, Advance Article
DOI: 10.1039/C3AN00303E

An in situ spatially resolved analytical technique to simultaneously probe gas phase reactions and temperature within the packed bed of a plug flow reactor
Jamal Touitou ,  Robbie Burch ,  Christopher Hardacre ,  Colin McManus ,  Kevin Morgan ,  Jacinto Sá and Alexandre Goguet
Analyst, 2013, Advance Article
DOI: 10.1039/C3AN00250K

Detection of cardiac troponin with a surface acustive wave immunosensor

Youn-Suk Choi and Soo Suk Lee from the Samsung Advanced Institute of Technology, Republic of Korea, have developed a point of care diagnostic test for cardiac troponin I (cTnI).

The researchers fabricated a bubble-free microfluidics device based on a gold-nanoparticle immunoassay and a surface acoustic wave immunosensor. The authors highlight a limit of detection of  6.7 pg mL-1 as a clinically relevant result and a first for centrifugally-based microfluidic devices. In this work, the researchers developed the device for the detection of cTnI, a biomarker for acute myocardial infarction for which rapid analysis would be beneficial for patient prognosis. The group is now extending studies to alternative systems.

To read more about the current results, access this Analyst HOT article clicking on the link below. This paper will be free to read for the next 10 days.

A centrifugally actuated point-of-care testing system for the surface acoustic wave immunosensing of cardiac troponin I
Woochang Lee ,  Jaeyeon Jung ,  Young Ki Hahn ,  Sang Kyu Kim ,  Yeolho Lee ,  Joonhyung Lee ,  Tae-Han Lee ,  Jin-Young Park ,  Hyejung Seo ,  Jung Nam Lee ,  Jin Ho Oh ,  Youn-Suk Choi and Soo Suk Lee
Analyst, 2013,138, 2558-2566
DOI: 10.1039/C3AN00182B

Enzyme electrodes for glucose biosensing

Glucose oxidase (GOx) is responsible for breaking down glucose and produces a detectable secondary signal used in a variety of biological assays. The primary electrical response from this catalytic process is difficult to detect because of the distance of the signal from within the enzyme to the electrode. To overcome these limitations, Jingquan Liu and researchers at the Qingdao University, China, have developed an enzyme-based electrode with pyrene functionalized GOx, which self assembles onto graphene sheets. The large surface area and high conductivity of graphene sheets enhance the electron transfer from the GOx via interaction with the pyrene, down to the electrode. In addition, increasing alternating layers of the pyrene-GOx and graphene enhances the biocatalytic activity in glucose solutions.

To know more about this research, please access the link below. This paper will be free to read until April 12^th.

Graphene bridged enzyme electrodes for glucose biosensing application
Jingquan Liu ,  Na Kong ,  Aihua Li ,  Xiong Luo ,  Liang Cui ,  Rui Wang and Shengyu Feng
Analyst, 2013, Advance Article
DOI: 10.1039/C3AN36929C

Take a look at these selected HOT articles just published in Analyst. Analysis of skin damage due to solar radiation, a new technique enabling “condom brand spectroscopic fingerprint” and the development of a testing system for the surface acoustic wave immunosensing of cardiac troponin I are among the HOT topics reported in Analyst. All the papers below will be free for you to read until April 12^th. Have a read now!

Raman spectroscopic mapping for the analysis of solar radiation induced skin damage
S. M. Ali ,  F. Bonnier ,  K. Ptasinski ,  H. Lambkin ,  K. Flynn ,  F. M. Lyng and H. J. Byrne
Analyst, 2013, Advance Article
DOI: 10.1039/C3AN36617K

Probing site-exclusive binding of aqueous QDs and their organelle-dependent dynamics in live cells by single molecule spectroscopy
Chaoqing Dong ,  Basudev Chowdhury and Joseph Irudayaraj
Analyst, 2013, Advance Article
DOI: 10.1039/C3AN36906D

Spectroscopic imaging based approach for condom identification in condom contaminated fingermarks
Robert Bradshaw ,  Rosalind Wolstenholme ,  Leesa Susanne Ferguson ,  Chris Sammon ,  Kerstin Mader ,  Emmanuelle Claude ,  Robert D. Blackledge ,  Malcolm R. Clench and Simona Francese
Analyst, 2013, Advance Article
DOI: 10.1039/C3AN00195D

Surface acoustic wave immunosensing of cardiac troponin I

Highly sensitive grating coupler-based surface plasmon-coupled emission (SPCE) biosensor for immunoassay
Jong Seol Yuk ,  Ernest F. Guignon and Michael A. Lynes
Analyst, 2013, Advance Article
DOI: 10.1039/C3AN00135K

Graphene bridged enzyme electrodes for glucose biosensing application
Jingquan Liu ,  Na Kong ,  Aihua Li ,  Xiong Luo ,  Liang Cui ,  Rui Wang and Shengyu Feng
Analyst, 2013, Advance Article
DOI: 10.1039/C3AN36929C

A centrifugally actuated point-of-care testing system for the surface acoustic wave immunosensing of cardiac troponin I
Woochang Lee ,  Jaeyeon Jung ,  Young Ki Hahn ,  Sang Kyu Kim ,  Yeolho Lee ,  Joonhyung Lee ,  Tae-Han Lee ,  Jin-Young Park ,  Hyejung Seo ,  Jung Nam Lee ,  Jin Ho Oh ,  Youn-Suk Choi and Soo Suk Lee
Analyst, 2013, Advance Article
DOI: 10.1039/C3AN00182B

Suwan Jayasinghe, Analyst, 2013, 138, 2215-2223

The Issue 8 of Analyst is now online. Take a look at our beautiful covers and discover all about the latest research in the field of analytical chemistry.

The outside front cover introduces the interesting minireview written by Suwan Jayasinghe from the University College London, featuring the most recent tools developed to generate fibers, scaffolds and membranes for application in regenerative biology and medicine. According to the author, these structures have important implications in both the laboratory and clinical settings, as they mimic the native extracellular matrix and can be used to directly reconstruct a three-dimensional functional tissue.

Cell electrospinning: a novel tool for functionalising fibres, scaffolds and membranes with living cells and other advanced materials for regenerative biology and medicine
Suwan N. Jayasinghe
Analyst, 2013,138, 2215-2223
DOI: 10.1039/C3AN36599A

Chen at al., Analyst, 2013, 138, 2392-2397

Our inside front cover features the work of Zhong-Ping Yao and colleagues from the Hong Kong Polytechnic University, who reported a new tool for detection and quantitation of ketamine and norketamine. In this study, drugs are detected in urine and oral fluids using wooden-tip electrospray ionization mass spectrometry (WT-ESI-MS). Compared to other methods, this approach has the advantage of little sample preparation and no chromatographic separation.

Rapid detection and quantitation of ketamine and norketamine in urine and oral fluid by wooden-tip electrospray ionization mass spectrometry
Pui-Kin So ,  Tsz-Tsun Ng ,  Haixing Wang ,  Bin Hu and Zhong-Ping Yao
Analyst, 2013,138, 2239-2243
DOI: 10.1039/C3AN36641C

Along with our two new covers, we have some HOT articles for you to enjoy. To know more, click on the links below. All these papers will be free to read until April 4^th.

Near-infrared to near-infrared upconverting NaYF4:Yb3+,Tm3+ nanoparticles-aptamer-Au nanorods light resonance energy transfer system for the detection of mercuric(II) ions in solution
Hong-Qi Chen ,  Fei Yuan ,  Shao-Zhen Wang ,  Juan Xu ,  Yi-Yan Zhang and Lun Wang
Analyst, 2013,138, 2392-2397
DOI: 10.1039/C3AN36921H

Determination of nitrite and glucose in water and human urine with light-up chromogenic response based on the expeditious oxidation of 3,3′,5,5′-tetramethylbenzidine by peroxynitrous acid
Jia Zhang ,  Cheng Yang ,  Chuanxia Chen and Xiurong Yang
Analyst, 2013,138, 2398-2404
DOI: 10.1039/C3AN00080J

Corona discharge radical emission spectroscopy: a multi-channel detector with nose-type function for discrimination analysis
Yunfei Tian ,  Peng Wu ,  Xi Wu ,  Xiaoming Jiang ,  Kailai Xu and Xiandeng Hou
Analyst, 2013,138, 2249-2253
DOI: 10.1039/C3AN36859A

Reversible binding and quantification of heparin and chondroitin sulfate in water using redox-stable biferrocenylene SAMs
Kun Chen and Michael Schmittel
Analyst, 2013,138, 2405-2410
DOI: 10.1039/C3AN36781A

Porphyrin-anion transmembrane transport using PAMPA

The Parallel Artificial Membrane Permeability Assay (PAMPA) is a popular tool used in pharmaceutical industry to screen new drugs and evaluate their permeability across membranes. Vladimir Kral and colleagues from the Institute of Chemical Technology Prague, propose a low cost approach for screening transmembrane transport of supermolecular complexes using PAMPA. According to the authors, inorganic anions bound to a porphyrin receptor can be efficiently transported across membranes.

To know more about the study, please click on the link below. This paper will be free to read until April 4^th.

Study of receptor mediated selective anion transmembrane transport using parallel artificial membrane permeability assay
Lenka Veverková ,  Kamil Záruba and Vladimír Král
Analyst, 2013, Advance Article
DOI: 10.1039/C3AN36760F

Photomicrograph of a nifedipine particle and photermal spectra

Duncan Craig from the University College London and collaborators from the Universities of Cambridge and East Anglia have applied Photothermal Infrared Microscopy (PTMS) to study drug degradation.

A combination of Fourier Transform Infrared Spectroscopy (FT-IR) and a modification of atomic force microscopy, PTMS, was used to pick up microparticles of a drug known for its photo-instability and analyse its degradation by changes in the FT-IR spectrum after exposure to light.  In this HOT new Analyst paper the researchers not only show the application of this new technique but discuss some limitations and areas for future development.

To know more about the study, please access the link below. This paper will be free to read until April 4^th.

Analysis of single particle photodegradation using photothermal infrared microspectroscopy
Jonathan G. Moffat ,  Mark D. Eddleston ,  Peter S. Belton ,  William Jones and Duncan Q. M. Craig
Analyst, 2013, Advance Article
DOI: 10.1039/C3AN36686C