Analyst Blog

FTIR micro-spectral images to monitor cell division

Take a look at our New HOT Articles free to read until June 3^rd. Click on the links below and discover all about the latest research published in Analyst:

Employing aqueous CdTe quantum dots with diversified surface functionalities to discriminate between heme (Fe(II)) and hemin (Fe(III))
Jishu Han, Ziwei Zhou, Xinyuan Bu, Shoujun Zhu, Hao Zhang, Haizhu Sun and   Bai Yang 
Analyst, 2013, Advance Article
DOI: 10.1039/C3AN00310H

A disposable electrochemical immunosensor for the determination of leptin in serum and breast milk
Irene Ojeda, María Moreno-Guzmán, Araceli González-Cortés, Paloma Yáñez-Sedeño and   José M. Pingarrón 
Analyst, 2013, Advance Article
DOI: 10.1039/C3AN00183K

Microfluidic assay to monitor anticoagulation therapy

Mepanipyrim haptens and antibodies with nanomolar affinity
Francesc Albert Esteve-Turrillas,   Josep Vicent Mercader,   Consuelo Agulló,   Antonio Abad-Somovilla and   Antonio Abad-Fuentes  
Analyst, 2013, Advance Article
DOI: 10.1039/C3AN00228D

Exploring the spectroscopic differences of Caki-2 cells progressing through the cell cycle while proliferating in vitro
M. Jimenez-Hernandez, C. Hughes, P. Bassan, F. Ball, M. D. Brown, N. W. Clarke and   P. Gardner
Analyst, 2013, Advance Article
DOI: 10.1039/C3AN00507K

A microfluidic anti-Factor Xa assay device for point of care monitoring of anticoagulation therapy
Leanne F. Harris, Paul Rainey, Vanessa Castro-López, James S. O’Donnell and   Anthony J. Killard
Analyst, 2013, Advance Article
DOI: 10.1039/C3AN00401E

Researchers have adapted a gold electrode to enhance electrochemical dopamine measurements and overcome the fouling problems that typically occur on the surface when using this technique.

Cells on doped overoxidised PPy modified electrodes

Many diseases such as Parkinson’s and schizophrenia are caused by irregularities in the neurotransmitter dopamine. Each disease affects dopamine production differently, however in all cases studying the process both inside and outside the body has presented many challenges.

Jenny Emnéus at the Technical University of Denmark and collaborators in Italy improved the detection of dopamine by placing a doped overoxidised polypyrrole (PPy) film on the electrode surface. The film was doped with different counter ions to inhibit dopamine polymerisation and the binding of negatively charged species. Although the overoxidation of PPy did affect the conductivity of the film, it also became more sensitive to dopamine, suggesting that doped overoxidised PPy can be used as sensors for dopamine.

To learn more about the techniques the authors used to reduce surface fouling and detect dopamine release from live cells, check out the article below. It will be free to read until May 28^th .

Doped overoxidized polypyrrole microelectrodes as sensors for the detection of dopamine released from cell populations
Luigi Sasso, Arto Heiskanen, Francesco Diazzi, Maria Dimaki, Jaime Castillo-León, Marco Vergani, Ettore Landini, Roberto Raiteri, Giorgio Ferrari, Marco Carminati, Marco Sampietro, Winnie E. Svendsen and   Jenny Emnéus  
Analyst, 2013, Advance Article
DOI: 10.1039/C3AN00085K

Visible image, infrared image, propidium iodide fluorescence image of a cell smear

Erik Goormaghtigh and co-workers from the Centre for Structural Biology and Bioinformatics at the Unversité Libre de Bruxelles have investigated the use of Fourier transformfo infrared (FTIR) spectroscopy r studying the effect of an anti-cancer drug, paclitaxel, on a human prostate cancer cell line.

The researchers combined fluorescence spectroscopy with FTIR spectroscopy using a fluorescent DNA intercalator to identify cell cycle stage.  By doing so, they sought to understand the drug’s effect on cell cycle and/or metabolic perturbation. The authors discuss both the application and limitation of this approach to cell studies.

Read more about this study in this HOT Analyst paper, free for you until May 24^th .

FTIR spectral signature of anticancer drug effects on PC-3 cancer cells: is there any influence of the cell cycle?
Allison Derenne, Alix Mignolet and   Erik Goormaghtigh  
Analyst, 2013, Advance Article
DOI: 10.1039/C3AN00225J

Synthesis, characterisation, tomography imaging of bismuth sulfide nanoparticles

Take a look at our HOT papers now available online . Tomography imaging applications, serum micro-Raman spectroscopy and photoelectrochemical studies are among the topics featured in these articles.

To read the full papers, please access the links below. They will be free for you until  May 20th.

Dendrimer-stabilized bismuth sulfide nanoparticles: synthesis, characterization, and potential computed tomography imaging applications
Yi Fang, Chen Peng, Rui Guo, Linfeng Zheng, Jinbao Qin, Benqing Zhou, Mingwu Shen, Xinwu Lu, Guixiang Zhang and   Xiangyang Shi  
Analyst, 2013, Advance Article
DOI: 10.1039/C3AN00237C

Diagnosis of hepatocellular carcinoma in cirrhotic patients: a proof-of-concept study using serum micro-Raman spectroscopy
I. Taleb, G. Thiéfin, C. Gobinet, V. Untereiner, B. Bernard-Chabert, A. Heurgué, C. Truntzer, P. Hillon, M. Manfait, P. Ducoroyc and   G. D. Sockalingum  
Analyst, 2013, Advance Article
DOI: 10.1039/C3AN00245D

Carbon nanotubes biosensors for malondialdehyde detection

Tagging the rolling circle products with nanocrystal clusters for cascade signal increase in the detection of miRNA
Jingjing Yao, Kenneth Flack, Liangzi Ding and   Wenwan Zhong
Analyst, 2013, Advance Article
DOI: 10.1039/C3AN00398A

Label-free and facile electrochemical biosensing using carbon nanotubes for malondialdehyde detection
Ling Yuan, Yaqian Lan, Min Han, Jianchun Bao, Wenwen Tu and   Zhihui Dai
Analyst, 2013, Advance Article
DOI: 10.1039/C3AN00041A

Tailoring zinc porphyrin to the Ag nanostructure substrate: an effective approach for photoelectrochemical studies in the presence of mononucleotides
Farkhondeh Fathi, Cong Kong, Yueqiang Wang, Yongshu Xie, Yi-Tao Long and   Heinz-Bernhard Kraatz  
Analyst, 2013, Advance Article
DOI: 10.1039/C3AN00156C

Isotachophoresis with ionic spacer and two-stage separation for high sensitivity DNA hybridization assay
Charbel Eid, Giancarlo Garcia-Schwarz and   Juan G. Santiago 
Analyst, 2013, Advance Article
DOI: 10.1039/C3AN00374D

Biological and biomedical 14C-accelerator mass spectrometry and graphitization of carbonaceous samples
Ill-Min Chung and   Seung-Hyun Kim
Analyst, 2013, Advance Article
DOI: 10.1039/C3AN00077J

An AC electrokinetics facilitated biosensor cassette for rapid pathogen identification
Mengxing Ouyang, Ruchika Mohan, Yi Lu, Tingting Liu, Kathleen E. Mach, Mandy L. Y. Sin, Mason McComb, Janhvi Joshi, Vincent Gau, Pak Kin Wong and   Joseph C. Liao 
Analyst, 2013, Advance Article
DOI: 10.1039/C3AN00259D

Visible and FTIR images showing plaques and lipids in mice tissues

Alzheimer’s disease (AD) affects millions of patients worldwide, and with much still unknown, few treatments options are available for the long duration of this disease. Although opinions differ on the cause of AD, histological staining shows the presence of neurotoxic plaques and tangles in deceased patients and serves as the primary diagnostic method.

Researchers at the University of Manitoba, Canada, used high resolution Fourier Transform Infrared Spectroscopy (FTIR) to image brain tissue in mice and human samples in order to study plaque formation at the sub cellular level. Different components in the cell such as DNA, proteins or sugars, produce unique chemical signatures which correspond to vibrational bands. The researchers discovered infiltration of lipid membrane components surrounding the plaques, which increase the signal as the plaques become larger. The spatial resolution of the system used here enables detection of lipids that a staining would miss, but it is important to understand disease progression. Although the relationship between plaque formation and lipid concentration remains unclear, the authors have developed a new method towards understanding plaque formation in AD.

To read the full article, please access the link below. This paper will be free to read until May 14^th .

Synchrotron FTIR reveals lipid around and within amyloid plaques in transgenic mice and Alzheimer’s disease brain
Catherine R. Liao, Margaret Rak, Jillian Lund, Miriam Unger, Eric Platt, Benedict C. Albensi, Carol J. Hirschmugl and   Kathleen M. Gough
Analyst, 2013, Advance Article
DOI: 10.1039/C3AN00295K

Enjoy our new HOT papers free for you to read until May 15^th. Click on the links below:

Analyst Issue 10

Study of receptor mediated selective anion transmembrane transport using parallel artificial membrane permeability assay
Lenka Veverková, Kamil Zárubaa and   Vladimír Král
Analyst, 2013,138, 2804-2807
DOI: 10.1039/C3AN36760F

LED-induced in-column molecular imprinting for solid phase extraction/capillary electrophoresis
Xinfeng Zhang, Shuxia Xu, Yong-Ill Lee and   Steven A. Soper
Analyst, 2013,138, 2821-2824
DOI: 10.1039/C3AN00257H

Radical induced disulfide bond cleavage within peptides via ultraviolet irradiation of an electrospray plume
Craig A. Stinson and   Yu Xia
Analyst, 2013,138, 2840-2846
DOI: 10.1039/C3AN00303E

Detection of factor IX using PEG-based blocking agents

A dual channel optical detector for trace water chemodosimetry and imaging of live cells
Guangwen Men, Guirong Zhang, Chunshuang Liang, Huiling Liu, Bing Yang, Yuyu Pan, Zhenyu Wang and   Shimei Jiang
Analyst, 2013,138, 2847-2857
DOI: 10.1039/C3AN36887D

An in situ spatially resolved analytical technique to simultaneously probe gas phase reactions and temperature within the packed bed of a plug flow reactor
Jamal Touitou, Robbie Burch, Christopher Hardacre, Colin McManus, Kevin Morgan, Jacinto Sá and   Alexandre Goguet
Analyst, 2013,138, 2858-2862
DOI: 10.1039/C3AN00250K

A high-performance waveguide-mode biosensor for detection of factor IX using PEG-based blocking agents to suppress non-specific binding and improve sensitivity
Thangavel Lakshmipriya, Makoto Fujimaki, Subash C. B. Gopinath, Koichi Awazu, Yukichi Horiguchi and   Yukio Nagasaki  
Analyst, 2013,138, 2863-2870
DOI: 10.1039/C3AN00298E

Probing site-exclusive binding of aqueous QDs and their organelle-dependent dynamics in live cells by single molecule spectroscopy
Chaoqing Dong, Basudev Chowdhury and   Joseph Irudayaraj 
Analyst, 2013,138, 2871-2876
DOI: 10.1039/C3AN36906D

A thin-layered chromatography plate prepared from naphthalimide-based receptor immobilized SiO2 nanoparticles as a portable chemosensor and adsorbent for Pb2+
Sunhong Park, Jin Hyeok Lee and   Jong Hwa Jung  
Analyst, 2013,138, 2812-2815
DOI: 10.1039/C3AN00292F

Chemical interactions and fate of nanoprobes upon entering living cells

Take a look at our new HOT papers free for you to read until May 13^th !

Do you know what happens to nanoprobes once they enter living cells? And did you hear about  in-column molecularly imprinted solid phase extraction concentrators for capillary electrophoresis?

To know more about the latest research published in Analyst, click on the links below and enjoy the reading!

LED-induced in-column molecular imprinting for solid phase extraction/capillary electrophoresis
Xinfeng Zhang ,  Shuxia Xu ,  Yong-Ill Lee and Steven A. Soper
Analyst, 2013, Advance Article
DOI: 10.1039/C3AN00257H

New light emitting diode polymerization technology

A thin-layered chromatography plate prepared from naphthalimide-based receptor immobilized SiO2 nanoparticles as a portable chemosensor and adsorbent for Pb2+
Sunhong Park ,  Jin Hyeok Lee and Jong Hwa Jung
Analyst, 2013, Advance Article
DOI: 10.1039/C3AN00292F

Off-line reaction monitoring of the oxidation of alkenes in water using drop coating deposition Raman (DCDR) spectroscopy
Shaghayegh Abdolahzadeh ,  Nicola M. Boyle ,  Apparao Draksharapu ,  Andrew C. Dennis ,  Ronald Hage ,  Johannes W. de Boer and Wesley R. Browne
Analyst, 2013, Advance Article
DOI: 10.1039/C3AN00330B

Probing site-exclusive binding of aqueous QDs and their organelle-dependent dynamics in live cells by single molecule spectroscopy
Chaoqing Dong,a   Basudev Chowdhurya and   Joseph Irudayaraj*a  
Analyst, 2013, Advance Article
DOI: 10.1039/C3AN36906D

FTIR based classification of carcinoma regions in brain tissue

Cancer is one of the top causes of death in the world, particularly for developed countries. Regardless of the cancer type, up to 40% of all cases metastasize to the brain during disease progression. Indeed, better survival rates are possible with early and accurate cancer detection.

While histopathologic evaluation involving staining of brain tissue is the current gold standard method for diagnosis, major drawbacks include the complexity of analysis and the non-specific staining of some dyes for non-cancerous cells. Moreover, histopathological staining, and other screening methods often cannot identify the primary tumor of brain metastasis. Without knowledge of the cancer origin, determination of the optimal treatment strategy can be difficult.

Christoph Krafft and colleagues from the Institute of Photonic Technology of Jena, Germany, have developed a strategy to help identify the primary tumor by using Fourier transform infrared (FTIR) and associated software. By analysing brain metastasis tissue, the “molecular fingerprint”, or vibrational spectra characteristic of the primary tumor can be found to deduce the cancer source.

Learn more about this exciting discovery by accessing the link below. This paper will be free to read until April 29^th:

Tumor margin identification and prediction of the primary tumor from brain metastases using FTIR imaging and support vector machines
Norbert Bergner ,  Bernd F. M. Romeike ,  Rupert Reichart ,  Rolf Kalff ,  Christoph Krafft and Jürgen Popp
Analyst, 2013, Advance Article
DOI: 10.1039/C3AN00326D

Take a look at our new HOT papers free for you to discover all about!
They cover a wide range of topics including the development a ruthenium-based smart probe for selective recognition of cations using a single optical technique and the optimization of a neuraminidase assay successfully integrated into a single-step operation. To read the full papers, please access the links below.
They will be free to read until April 29th.

A stimuli-responsive “smart probe” for selective monitoring of multiple-cations via differential analyses
Anup Kumar ,  Alok K. Singh and Tarkeshwar Gupta
Analyst, 2013, Advance Article
DOI: 10.1039/C3AN00087G

A dual channel optical detector for trace water chemodosimetry and imaging of live cells
Guangwen Men ,  Guirong Zhang ,  Chunshuang Liang ,  Huiling Liu ,  Bing Yang ,  Yuyu Pan ,  Zhenyu Wang and Shimei Jiang
Analyst, 2013, Advance Article
DOI: 10.1039/C3AN36887D

Spectral cytopathology: new aspects of data collection, manipulation and confounding effects
Miloš Miljković ,  Benjamin Bird ,  Kathleen Lenau ,  Antonella I. Mazur and Max Diem
Analyst, 2013, Advance Article
DOI: 10.1039/C3AN00185G

Integration of neuraminidase inhibitor assay into a single-step operation using a combinable poly(dimethylsiloxane) capillary sensor
Tadashi Ishimoto ,  Kaede Jigawa ,  Terence G. Henares ,  Tatsuro Endo and Hideaki Hisamoto
Analyst, 2013, Advance Article
DOI: 10.1039/C3AN36785A

A high-performance waveguide-mode biosensor for detection of factor IX using PEG-based blocking agents to suppress non-specific binding and improve sensitivity
Thangavel Lakshmipriya ,  Makoto Fujimaki ,  Subash C. B. Gopinath ,  Koichi Awazu ,  Yukichi Horiguchi and Yukio Nagasaki
Analyst, 2013, Advance Article
DOI: 10.1039/C3AN00298E

Take a look at the HOT papers recently published in Analyst! They include a wide variety of topics, from the use of Raman spectroscopy for detection of cancer and malaria to the development of new analytical techniques to probe gas phase reactions and temperatures. These papers will be free to read until April 19th. Have a read now!

SERS analysis blood test for non invasive cervical cancer detection

Blood plasma surface-enhanced Raman spectroscopy for non-invasive optical detection of cervical cancer
Shangyuan Feng ,  Duo Lin ,  Juqiang Lin ,  Buhong Li ,  Zufang Huang ,  Guannan Chen ,  Wei Zhang ,  Lan Wang ,  Jianji Pan ,  Rong Chen and Haishan Zeng
Analyst, 2013, Advance Article
DOI: 10.1039/C3AN36890D

Raman spectroscopic analysis of malaria disease progression via blood and plasma samples
Alison J. Hobro ,  Aki Konishi ,  Cevayir Coban and Nicholas I. Smith
Analyst, 2013, Advance Article
DOI: 10.1039/C3AN00255A

Raman spectroscopiuc analysis of malaria disease

Radical induced disulfide bond cleavage within peptides via ultraviolet irradiation of an electrospray plume
Craig A. Stinson and Yu Xia
Analyst, 2013, Advance Article
DOI: 10.1039/C3AN00303E

An in situ spatially resolved analytical technique to simultaneously probe gas phase reactions and temperature within the packed bed of a plug flow reactor
Jamal Touitou ,  Robbie Burch ,  Christopher Hardacre ,  Colin McManus ,  Kevin Morgan ,  Jacinto Sá and Alexandre Goguet
Analyst, 2013, Advance Article
DOI: 10.1039/C3AN00250K