The potential of microchip electrophoresis for the rapid and highly sensitive analysis of mRNA expression in cells by using a Cy5-labeled antisense riboprobe in the RNA protection assay has been shown by researchers from Japan.
Shouki Yatsushiro and colleagues from National Institute of Advanced Industrial Science and Technology (AIST), Takamatsu, show that results can be obtained in 120 seconds, using the same amount of riboprobe as used in the conventional method, with eight-times more sensitive detection of mRNA.
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Ribonuclease protection assay on microchip electrophoresis
Yuka Yamaguchi, Shouki Yatsushiro, Shohei Yamamura, Hiroko Abe, Kaori Abe, Masahiro Watanabe, Kazuaki Kajimoto, Yasuo Shinohara, Yoshinobu Baba and Masatoshi Kataoka
Analyst, 2011, Advance Article
DOI: 10.1039/C0AN01044H
