Archive for February, 2014

Shedding New Light on Old Treasures

Analytical techniques are shedding new light on old treasures.

Madame Leon Clapisson, 1883

Analytical chemists are making breakthroughs in the science of heritage and conservation. Using new techniques, previously lost nuances in the works of cultural artifacts are being rediscovered, enriching our understanding of our shared culture, and recasting our relationship with the past.

Dr Richard P. Van Duyne recently used Raman spectroscopy to reveal the stunning original colours of Renoir’s “Madame Valentine Clapisson” painted over a century ago. Using Surface Enhanced Raman Spectroscopy (SERS), a technique widely used in biomedical research that Van Duyne has spent the past few years adapting for the study of organic paints, the lush reds of the origiGraphical abstract: Mapping of egg yolk and animal skin glue paint binders in Early Renaissance paintings using near infrared reflectance imaging spectroscopynal could be acurately recreated. SERS involves adsorbing tiny paint samples to metal nanoparticles, which enhances the Raman signal so trace amounts of the pigment molecules can be detected. Routine art analysis techniques like x-ray fluorescence scanning are unsuitable for this kind of paint, as they rely on elemental signatures from metals, without Dr Van Duyne’s developments the original colours envisioned by Renoir would remain unknown.

In a recent themed collection in Analyst and Analytical Methods cutting edge developments in analytical science used in heritage and conservation were drawn together. Guest edited by Dr Francesca Casadio and Dr Richard P. Van Duyne, the collection of articles highlights work on historic buildings, illuminated manuscripts and paintings by artists such as Dali and Matisse, as well as developments on the underpinning analytical techniques that are revealing ground-breaking insights into our rich cultural heritage.

In their editorial, Dr Francesca Casadio and Dr Richard P. Van Duyne set out the case for the cooperation between the chemical sciences and heritage work, and highlight the need for future research into this area to guarantee the conservation and understanding of our shared history.

The collection is open for submissions. Submit your work to the collection, or read it online today.

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SPEC 2014

logo

The aim of this international conference series is to bring together clinicians and scientists who have joined forces in the quest for novel biomedical applications of Infrared and Raman spectroscopy to improve patient care. Recent advances in the biological sciences and medicine have led to an increasing demand for real time and minimally invasive chemical and structural information on biological materials. Due to its unique fingerprinting capability, vibrational spectroscopy plays a significant role in histopathology, cytology, biopsy targeting, surgical targets, treatment monitoring and drug studies. This, the 8th in the series, aims to highlight further advances in state of the art and emerging biomedical applications of Vibrational Spectroscopy, while critically reviewing the challenges in the context of other emerging technologies.

Date: 17-22 August 2014
Location: Krakow, Poland
Contact: Katarzyna Marzec
Email: marzec@chemia.uj.edu.pl
Website

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Novel analytical technique for profiling enteric coated drugs

An image of enteric coated drug delivery spheres dissolving while being profiled by Broadband Acoustic Resonance Dissolution Spectroscopy (BARDS)

The authors work features on the front cover of Issue 5

This blog has been written by Adam Davis, a Guest Web-Writer for Analyst and Analytical Methods.

Enteric coatings are used to protect acid labile active pharmaceutical ingredients upon entry into the stomach, allowing for controlled and sustained release of active ingredients to the small intestine later. Enteric coated formulations typically consist of a sugar core, a layer of active ingredient followed by the enteric coating. Using current analysis methods determining the thickness of these layers can take up to 24hrs.

In this article, which appears in Issue 5 of Analyst, researchers from University College Cork, Ireland and Kinetox, Netherlands, have demonstrated how Broadband Acoustic Resonance Dissolution Spectroscopy (BARDS) can be used for rapid profiling of enteric coated spheres. The BARDS technique is a novel approach which utilises the acoustic signature of cores spheres and enteric coating as they dissolve, which arise as result of the changes in compressibility of the solvent as a compound dissolves in it. This alters the speed of sound through the solvent, which in turn changes the frequency in solution. The use of BARDS has important implications for manufacturing, quality assurance and stability testing for pharmaceuticals.

Rapid profiling of enteric coated drug delivery spheres via Broadband Acoustic Resonance Dissolution Spectroscopy (BARDS)
D. Fitzpatrick, R. Evans-Hurson, Y. Fu, T. Burke, J. Krüse, B. Vos, S. G. McSweeney, P. Casaubieilh and J. J. Keating
Analyst, 2014, 139, 1000-1006
DOI: 10.1039/C3AN01809A

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HOT articles in Analyst

Take a look at our new HOT articles just published in Analyst and free for you for the next couple of weeks: Graphical abstract: An antibody-free microfluidic paper-based analytical device for the determination of tear fluid lactoferrin by fluorescence sensitization of Tb3+

An antibody-free microfluidic paper-based analytical device for the determination of tear fluid lactoferrin by fluorescence sensitization of Tb3+
Kentaro Yamada, Shunsuke Takaki, Nobutoshi Komuro, Koji Suzuki and Daniel Citterio
Analyst, 2014, Advance Article
DOI: 10.1039/C3AN01926H, Paper

Protein binding for detection of small changes on a nanoparticle surface
Shang Zeng, Yu-ming M. Huang, Chia-en A. Chang and Wenwan Zhong
Analyst, 2014,139, 1364-1371
DOI: 10.1039/C3AN02155F, Paper

Proteomic evaluation of citrate-coated silver nanoparticles toxicity in Daphnia magna
Louis-Charles Rainville, Darragh Carolan, Ana Coelho Varela, Hugh Doyle and David Sheehan
Analyst, 2014, Advance Article
DOI: 10.1039/C3AN02160B, Paper

Oxidized polyethylene films for orienting polar molecules for linear dichroism spectroscopy
Kasra Razmkhah, Nikola Paul Chmel, Matthew I. Gibson and Alison Rodger
Analyst, 2014,139, 1372-1382
DOI: 10.1039/C3AN02322B, Paper Graphical abstract: Making a big thing of a small cell – recent advances in single cell analysis

Solid-phase extraction and purification of membrane proteins using a UV-modified PMMA microfluidic bioaffinity μSPE device
Katrina N. Battle, Joshua M. Jackson, Małgorzata A. Witek, Mateusz L. Hupert, Sally A. Hunsucker, Paul M. Armistead and Steven A. Soper
Analyst, 2014,139, 1355-1363
DOI: 10.1039/C3AN02400H, Paper

Non-invasive mouthguard biosensor for continuous salivary monitoring of metabolites
Jayoung Kim, Gabriela Valdés-Ramírez, Amay J. Bandodkar, Wenzhao Jia, Alexandra G. Martinez, Julian Ramírez, Patrick Mercier and Joseph Wang
Analyst, 2014, Advance Article
DOI: 10.1039/C3AN02359A, Paper

Making a big thing of a small cell – recent advances in single cell analysis
Kerstin Galler, Katharina Bräutigam, Christina Große, Jürgen Popp and Ute Neugebauer
Analyst, 2014,139, 1237-1273
DOI: 10.1039/C3AN01939J, Critical Review

Advances in nanowire transistors for biological analysis and cellular investigation
Bor-Ran Li, Chiao-Chen Chen, U. Rajesh Kumar and Yit-Tsong Chen
Analyst, 2014, Advance Article
DOI: 10.1039/C3AN01861J, Critical Review

High resolution magic angle spinning NMR as a tool for unveiling the molecular enantiorecognition of omeprazole by amylose-based chiral phase
Juliana Cristina Barreiro, Tiago de Campos Lourenço, Lorena Mara A. Silva, Tiago Venâncio and Quezia Bezerra Cass
Analyst, 2014,139, 1350-1354
DOI: 10.1039/C3AN02029K, Paper Graphical abstract: Intra-molecular reactions as a new approach to investigate bio-radical reactivity: a case study of cysteine sulfinyl radicals

Intra-molecular reactions as a new approach to investigate bio-radical reactivity: a case study of cysteine sulfinyl radicals
Kirt L. Durand, Xiaoxiao Ma and Yu Xia
Analyst, 2014,139, 1327-1330
DOI: 10.1039/C3AN02307A, Communication

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Top ten most accessed Analyst articles from October to December 2013

Here are the top ten most accessed Analyst articles for October, November and December 2013.

Imaging live cells grown on a three dimensional collagen matrix using Raman microspectroscopy
F. Bonnier, P. Knief, B. Lim, A. D. Meade, J. Dorney, K. Bhattacharya, F. M. Lyng and H. J. Byrne
Analyst, 2010,135, 3169-3177
DOI: 10.1039/C0AN00539H, Paper

Detection of calcium phosphate crystals in the joint fluid of patients with osteoarthritis – analytical approaches and challenges
Alexander Yavorskyy, Aaron Hernandez-Santana, Geraldine McCarthy and Gillian McMahon
Analyst, 2008,133, 302-318
DOI: 10.1039/B716791A, Critical Review

Gold-coated polycarbonate membrane filter for pathogen concentration and SERS-based detection
Krista Rule Wigginton and Peter John Vikesland
Analyst, 2010,135, 1320-1326
DOI: 10.1039/B919270K, Paper

Capillary zone electrophoresis-multiple reaction monitoring from 100 pg of RAW 264.7 cell lysate digest
Liangliang Sun, Yihan Li, Matthew M. Champion, Guijie Zhu, Roza Wojcik and Norman J. Dovichi
Analyst, 2013,138, 3181-3188
DOI: 10.1039/C3AN00287J, Paper

Upconversion nanoparticles in biological labeling, imaging, and therapy
Feng Wang, Debapriya Banerjee, Yongsheng Liu, Xueyuan Chen and Xiaogang Liu
Analyst, 2010,135, 1839-1854
DOI: 10.1039/C0AN00144A, Critical Review

Single-channel electrophysiology of cell-free expressed ion channels by direct incorporation in lipid bilayers
Mark S. Friddin, Natalie P. Smithers, Maïwenn Beaugrand, Isabelle Marcotte, Philip T. F. Williamson, Hywel Morgan and Maurits R. R. de Planque
Analyst, 2013,138, 7294-7298
DOI: 10.1039/C3AN01540H, Communication

A non enzymatic glucose biosensor based on an ultrasensitive calix[4]arene functionalized boronic acid gold nanoprobe for sensing in human blood serum
Alok Pandya, Pinkesh G. Sutariya and Shobhana K. Menon
Analyst, 2013,138, 2483-2490
DOI: 10.1039/C3AN36833E, Paper

Mid-infrared spectroscopy for gases and liquids based on quantum cascade technologies
Pierre Jouy, Markus Mangold, Béla Tuzson, Lukas Emmenegger, Yu-Chi Chang, Lubos Hvozdara, Hans Peter Herzig, Philip Wägli, Alexandra Homsy, Nico F. de Rooij, Alexander Wirthmueller, Daniel Hofstetter, Herbert Looser and Jérôme Faist
Analyst, 2014, Advance Article
DOI: 10.1039/C3AN01462B, Paper

A pyrene-based simple but highly selective fluorescence sensor for Cu2+ ions via a static excimer mechanism
Soma Sarkar, Swapnadip Roy, Anindita Sikdar, R. N. Saha and Sujit S. Panja
Analyst, 2013,138, 7119-7126
DOI: 10.1039/C3AN00928A, Paper

A novel 2,6-diformyl-4-methylphenol based chemosensor for Zn(II) ions by ratiometric displacement of Cd(II) ions and its application for cell imaging on human melanoma cancer cells
Atanu Jana, Pradip K. Sukul, Sushil K. Mandal, Saugata Konar, Sangita Ray, Kinsuk Das, James A. Golen, Arnold L. Rheingold, Sudipa Mondal, Tapan K. Mondal, Anisur R. Khuda-Bukhsh and Susanta K. Kar
Analyst, 2014,139, 495-504
DOI: 10.1039/C3AN01750H, Paper

We hope you enjoyed reading these articles, and if you have any thoughts or comments, please leave these in the comment box below.

Fancy submitting an article to Analyst? Why not submit to us here today or alternatively email us your suggestions!

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The Ongoing Hunt for Deadly Microbes – A New Lateral Flow Assay for Pathogen Detection

Early and accurate monitoring of pathogens such as Escherichia coli is critical for food and water safety, clinical diagnosis, and biosecurity. However there are a number of limitations to current detection methods such as cell culturing, and immunoassays. These drawbacks include non-specificity, insensitivity, as well as the requirement of long analysis time, intensive labor, and extensive sample purification. To address some of these limitations, Christopher Pöhlmann and his colleagues from the University of Bayreuth in Germany have developed a lateral flow device based on a sandwich assay design using gold nanoparticle-oligonucleotides to detect ribosomal RNA of E. coli. The new device is able to provide specific detection of E. coli within 25 minutes and requires no signal amplification step.

A Lateral Flow Assay for Detection of E. coli Ribosomal RNA

A Lateral Flow Assay for Detection of E. coli Ribosomal RNA

Find out more about this discovery by reading the full paper below, which is free to download until March 7th:

A lateral flow assay for identification of Escherichia coli by ribosomal RNA hybridization
Christopher Pöhlmann, Irina Dieser and Mathias Sprinzl
Analyst, 2014, 139, 1063-1071
DOI: 10.1039/C3AN02059B

Let us know what your thoughts are by leaving a comment!

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Saliva information

Scientists in the US have developed a mouthguard sensor that could be used to monitor metabolites in saliva to provide real-time information on the health status of the wearer.

Real-time health monitoring is an attractive goal for biomedical, sport and military fields. A range of sensors have been developed that monitor physical signs such as heart rate, but recently interest has moved to detecting biomarkers non-invasively in body fluids to obtain more detailed information.

Saliva is an easily obtainable body fluid and contains analytes that closely correlate to those of blood, providing a good indication of metabolism and hormone levels. Previous saliva sensors have included dental tattoos and dentures but the new mouthguard sensor from Joseph Wang and colleagues at the University of California, San Diego, is much less invasive.

To read the full article by Harriet Brewerton, please go to Chemistry World.

Non-invasive mouthguard biosensor for continuous salivary monitoring of metabolites
Jayoung Kim, Gabriela Valdés-Ramírez, Amay J. Bandodkar, Wenzhao Jia, Alexandra G. Martinez, Julian Ramírez, Patrick Mercier and Joseph Wang
Analyst, 2014, Advance Article
DOI: 10.1039/C3AN02359A, Paper

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11th European FTMS Conference

11th European FTMS Conference

Deadline for abstract submission: February 17th, 2014 (Midnight, CET)

This bi-annual conference aims to bring together leading academic and industrial scientists working in the field of state-of-the-art high resolution mass spectrometry – Ion Cyclotron Resonance and Orbitrap Fourier Transform Mass Spectrometry.

The program of the conference will cover all the aspects of FTMS from fundamentals and technical innovations to cutting-edge applications in the life and environmental sciences.

Topics:

FTMS and other High Resolution MS: Instrumentation
Top-down Mass Spectrometry
Data Analysis
Developments in biomolecular analysis
Post Translational Modifications
Complex mixture analysis
Imaging
Gas phase structure

Date: 22-25th April 2014
Location: Institut Pasteur, Paris
Contact: Ms Elodie Pysson
Email: eftms2014@pasteur.fr
Webiste: http://www.eftms2014.org/

Submit online here

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HOT articles in Analyst

Take a look at our new HOT articles just published in Analyst and free for you for the next couple of weeks:

reMelting curve analysis as a tool for enrichment monitoring in the SELEX process
Jeroen Vanbrabant, Karen Leirs, Katrijn Vanschoenbeek, Jeroen Lammertyn and Luc Michiels
Analyst, 2014,139, 589-595
DOI: 10.1039/C3AN01884A, Paper

Graphical abstract: reMelting curve analysis as a tool for enrichment monitoring in the SELEX process

Nanostructured silver–gold bimetallic SERS substrates for selective identification of bacteria in human blood
Arumugam Sivanesan, Evelin Witkowska, Witold Adamkiewicz, Łukasz Dziewit, Agnieszka Kamińska and Jacek Waluk
Analyst, 2014,139, 1037-1043
DOI: 10.1039/C3AN01924A, Paper

Graphical abstract: Nanostructured silver–gold bimetallic SERS substrates for selective identification of bacteria in human blood

Investigation of non-specific signals in nanoporous flow-through and flow-over based sensors
Neeraj Kumar, Elena Froner, Romain Guider, Marina Scarpa and Paolo Bettotti
Analyst, 2014, Advance Article
DOI: 10.1039/C3AN01996A, Paper

Graphical abstract: Investigation of non-specific signals in nanoporous flow-through and flow-over based sensors

Selective detection of complementarity-determining regions of monoclonal antibody by limiting protease access to the substrate: nano-surface and molecular-orientation limited proteolysis
Noriko Iwamoto, Takashi Shimada, Yukari Umino, Chikage Aoki, Yutaka Aoki, Taka-Aki Sato, Akinobu Hamada and Hitoshi Nakagama
Analyst, 2014,139, 576-580
DOI: 10.1039/C3AN02104A, Communication

Graphical abstract: Selective detection of complementarity-determining regions of monoclonal antibody by limiting protease access to the substrate: nano-surface and molecular-orientation limited proteolysis

A model system for targeted drug release triggered by biomolecular signals logically processed through enzyme logic networks
Shay Mailloux, Jan Halámek and Evgeny Katz
Analyst, 2014,139, 982-986
DOI: 10.1039/C3AN02162A, Communication

Graphical abstract: A model system for targeted drug release triggered by biomolecular signals logically processed through enzyme logic networks

Biocatalytic analysis of biomarkers for forensic identification of gender
Saira Bakshi, Lenka Halámková, Jan Halámek and Evgeny Katz
Analyst, 2014,139, 559-563
DOI: 10.1039/C3AN02055J, Communication

Graphical abstract: Biocatalytic analysis of biomarkers for forensic identification of gender

Real-time trace gas sensing of fluorocarbons using a swept-wavelength external cavity quantum cascade laser
Mark C. Phillips, Matthew S. Taubman, Bruce E. Bernacki, Bret D. Cannon, Robert D. Stahl, John T. Schiffern and Tanya L. Myers
Analyst, 2014, Advance Article
DOI: 10.1039/C3AN01642K, Paper

Graphical abstract: Real-time trace gas sensing of fluorocarbons using a swept-wavelength external cavity quantum cascade laser

Frequency-encoded laser-induced fluorescence for multiplexed detection in infrared-mediated quantitative PCR
Adrian M. Schrell and Michael G. Roper
Analyst, 2014, Advance Article
DOI: 10.1039/C3AN02334F, Paper

Graphical abstract: Frequency-encoded laser-induced fluorescence for multiplexed detection in infrared-mediated quantitative PCR

Rapid profiling of enteric coated drug delivery spheres via Broadband Acoustic Resonance Dissolution Spectroscopy (BARDS)
D. Fitzpatrick, R. Evans-Hurson, Y. Fu, T. Burke, J. Krüse, B. Vos, S. G. McSweeney, P. Casaubieilh and J. J. Keating
Analyst, 2014,139, 1000-1006
DOI: 10.1039/C3AN01809A, Paper

Graphical abstract: Rapid profiling of enteric coated drug delivery spheres via Broadband Acoustic Resonance Dissolution Spectroscopy (BARDS)

Highly enhanced electrochemiluminescence based on pseudo triple-enzyme cascade catalysis and in situ generation of co-reactant for thrombin detection
Lijuan Xiao, Yaqin Chai, Ruo Yuan, Haijun Wang and Lijuan Bai
Analyst, 2014,139, 1030-1036
DOI: 10.1039/C3AN02134C, Paper

Graphical abstract: Highly enhanced electrochemiluminescence based on pseudo triple-enzyme cascade catalysis and in situ generation of co-reactant for thrombin detection

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Label-free biosensing using photonic crystals and single nanoparticles

Photonic crystal enhanced microscopy (PCEM) is a relatively new technique that utilizes a grating like substrate composed of a high refractive index material coating a low refractive index one. When irradiated with light at a resonant wavelength, this photonic crystal reflects all of the light, and is very sensitive to any changes on the surface.

Photonic Crystal Enhanced Microscopy of a nanoparticle on a photonic crystal

Photonic Crystal Enhanced Microscopy of a nanoparticle on a photonic crystal

Researchers at the University of Illinois in the United States studied the effects of gold nanoparticles binding to  photonic crystals composed of a liquid epoxy polymer and TiO2.  Although PCEM has been used to detect biological analytes without labels, they observed how a nanoparticle tag can reduce  the signal of a photonic crystal. Furthermore, they determined the distance required between the nanoparticles for detection, and this could be used in future amplification schemes such as in single molecule sensing.

To read more about this topic, click the link below. It will be free to read until February 28.

Single nanoparticle detection using photonic crystal enhanced microscopy
Yue Zhuo,  Huan Hu, Weili Chen,  Meng Lu,  Limei Tian,  Hojeong Yu, Kenneth D. Long,  Edmond Chow,  William P.   King, Srikanth Singamaneni  and  Brian T. Cunningham
Analyst, 2014, Advance Article
DOI: 10.1039/C3AN02295A

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