Archive for 2012

HOT article: Protein binding to multiple DNA targets in a one-pot reaction

Moreau & Schaeffer, Analyst, 2012, 137, 4111

By Laura Ruvuna, Web Writer

Morgane Moreau and Patrick Schaeffer at James Cook University in Australia have developed a qPCR based binding assay for detecting protein-DNA complexes. Understanding these binding interactions is both biologically vital but difficult to study using traditional methods. Alternatively, this simple procedure requires GFP-Tus, a termination replication protein, a GFP antibody, and multiple strands of DNA in a single reaction. After the immunoprecipitation of the protein-DNA complex, qPCR measures the quantification of each strand separately in polyplex. In addition to being quick and cheap, this assay has the versatility of combining with other common techniques, such as SPR, to increase overall sensitivity. To read more about this group’s new detection method, check out the article below.

A polyplex qPCR-based binding assay for protein–DNA interactions
Morgane J. J. Moreau and Patrick M. Schaeffer
Analyst, 2012, 137, 4111-4113
DOI: 10.1039/C2AN35703H

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HOT articles in Analyst this August

Check out some of the HOT articles published this last month in Analyst that you may have missed. These papers will be free to access for 2 weeks!

Sensitive detection of H2O2 and H2O2-related reactant with Ru(bipy)2(7,8-dimethyl-dipyridophenazine)2+ and oligodeoxyribonucleotide
Cen Xiong, Zhiyou Xiao, Manjun Zhang and Liansheng Ling
Analyst, 2012, 137, 4428-4434
DOI: 10.1039/C2AN35519A

Ultrasensitive aptamer biosensor for arsenic(III) detection in aqueous solution based on surfactant-induced aggregation of gold nanoparticles
Yuangen Wu, Le Liu, Shenshan Zhan, Faze Wang and Pei Zhou
Analyst, 2012, 137, 4171-4178
DOI: 10.1039/C2AN35711A

Highly selective fluorescence turn-on sensing of gold ions by a nanoparticle generation/C–I bond cleavage sequence
Jihye Park, Sohee Choi, Tae-Il Kim and Youngmi Kim
Analyst, 2012, 137, 4411-4414
DOI: 10.1039/C2AN35351B

Zhou et al., Analyst, 2012, 137, 4171

Endonuclease V-assisted accurate cleavage of oligonucleotide probes controlled by deoxyinosine and deoxynucleoside phosphorothioate for sequencing-by-ligation
Yanqiang Li, Zhiqiang Pan, Jing Tang, Dan Pu, Pengfeng Xiao and Zuhong Lu
Analyst, 2012, 137, 4421-4424
DOI: 10.1039/C2AN35595G

Development of a pH-activatable fluorescent probe and its application for visualizing cellular pH change
Rong Huang, Shengyong Yan, Xiaolong Zheng, Fengling Luo, Minggang Deng, Boshi Fu, Yijie Xiao, Xi Zhao and Xiang Zhou
Analyst, 2012, 137, 4418-4420
DOI: 10.1039/C2AN35671F

Analysis of layered assays and volume microarrays in stratified media
Homanaz Ghafari and Quentin S. Hanley
Analyst, 2012, Advance Article
DOI: 10.1039/C2AN35767D

Ghafari & Hanley, Analyst, 2012, Advance Article

Assessing the responses of cellular proteins induced by hyaluronic acid-modified surfaces utilizing a mass spectrometry-based profiling system: Over-expression of CD36, CD44, CDK9, and PP2A
Ming-Hui Yang, Shiang-Bin Jong, Chi-Yu Lu, Yu-Fen Lin, Pei-Wen Chiang, Yu-Chang Tyan and Tze-Wen Chung
Analyst, 2012, Advance Article
DOI: 10.1039/C2AN35368G

Gold nanoparticles-based nanoconjugates for enhanced enzyme cascade and glucose sensing
Dongdong Zeng, Weijie Luo, Jiang Li, Huajie Liu, Hongwei Ma, Qing Huang and Chunhai Fan
Analyst, 2012, 137, 4435-4439
DOI: 10.1039/C2AN35900F

Functionalized shell-isolated nanoparticle-enhanced Raman spectroscopy for selective detection of trinitrotoluene
Kai Qian, Honglin Liu, Liangbao Yang and Jinhuai Liu
Analyst, 2012, Advance Article
DOI: 10.1039/C2AN35947B

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HOT articles in Analyst

Screening for D-amino-acid oxidase inhibitors

Qi et al., Analyst, 2012, 137, 4235

A team from China have been working on a capillary electrophoresis (CLE-CE) system for the enantioseparation of dansyl amino acids. Their method was tested on the inhibition efficiency of D-amino acid oxidase (DAAO) inhibitors, which could pave a the way for high-throughput screening of enzyme inhibitors and relevant drug discovery.

A novel chiral ligand exchange capillary electrophoresis system with amino acid ionic liquid as ligand and its application in screening D-amino-acid oxidase inhibitors
Xiaoyu Mu, Li Qi, Ying Shen, Haizhi Zhang, Juan Qiao and Huimin Ma
Analyst, 2012, 137, 4235-4240
DOI: 10.1039/C2AN35753D

A turn-on and reversible fluorescence sensor for zinc ion

Wu et al., Analyst, 2012, Advance Article

A new zinc sensor has been developed by a team working at the National Changhua University of Education and Chung Shan Medical University, Taiwan. Zinc is the second most abundant transition metal in the human body, and although it is considered to be relatively nontoxic, toxicity symptoms can occur with extremely high intake.

A turn-on and reversible fluorescence sensor for zinc ion
Hsiang-Yi Lin, Pi-Yun Cheng, Chin-Feng Wan and An-Tai Wu
Analyst, 2012, Advance Article
DOI: 10.1039/C2AN35752F

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HOT articles in Issue 18

lectrochemical impedance spectroscopy as novel technology for the management of patients with diabetes mellitus

Adamson et al., Analyst, 2012, 137, 4179-4187

A number of HOT papers feature in our latest issue, including a qPCR-based binding assay to measure protein–DNA interactions in polyplex, a comparison of the different modes of interrogation for surface plasmon resonance (SPR) sensors, and the development of electrochemical impedance spectroscopy to offer a unique frequency detection of glucose (and other targets) for better diabetes management.

All these papers will be free to access until 31 August.

Communication: A polyplex qPCR-based binding assay for protein–DNA interactions
Morgane J. J. Moreau and Patrick M. Schaeffer
Analyst, 2012, 137, 4111-4113
DOI: 10.1039/C2AN35703H

EOT or Kretschmann configuration? Comparative study of the plasmonic modes in gold nanohole arrays
Maxime Couture, Ludovic S. Live, Anuj Dhawan and Jean-Francois Masson
Analyst, 2012, 137, 4162-4170
DOI: 10.1039/C2AN35566C

The promise of electrochemical impedance spectroscopy as novel technology for the management of patients with diabetes mellitus
Teagan Leigh Adamson, Francis Ang Eusebio, Curtiss B. Cook and Jeffrey T. LaBelle
Analyst, 2012, 137, 4179-4187
DOI: 10.1039/C2AN35645G

See what else is HOT below.

(more…)

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Issue 18 now online

Analyst 2012, Issue 18, front cover

Front cover: Hong et al., Analyst, 2012, 137, 4140-4149

Our latest issue is now available, so do take a look.

Featured on the front cover, Qingdong Zheng from the Fujian Institute of Research on the Structure of Matter, Fuzhou, China, and co-workers, report on the synthesis and characterization of two silylated BODIPY dyes and their use in dye-encapsulated silica nanoparticles with switchable emitting wavelengths for cellular imaging. In order to realise multiplexed imaging, they doped two different dyes into the same nanoparticle, which is able to emit light with different colors based on a change in excitation wavelengths.

Silylated BODIPY dyes and their use in dye-encapsulated silica nanoparticles with switchable emitting wavelengths for cellular imaging
Xuehua Hong, Zhuyuan Wang, Jing Yang, Qingdong Zheng, Shenfei Zong, Yu Sheng, Deqin Zhu, Changquan Tang and Yiping Cui
Analyst, 2012, 137, 4140-4149
DOI: 10.1039/C2AN35389J

On the inside front cover, Natalie Cann and colleagues from Queen’s University, Kingston, Canada present full-dimensional computational fluid dynamics (CFD) simulations for nano electrospray ionization (ESI) with various emitter designs as a step towards an integrated approach to emitter design and characterization.

Analyst 2012, Issue 18, inside front cover

Inside front cover: Wu et al., Analyst, 2012, 137, 4150-4161

Characterization of microstructured fibre emitters: in pursuit of improved nano electrospray ionization performance
Xinyun Wu, Richard D. Oleschuk and Natalie M. Cann
Analyst, 2012, 137, 4150-4161
DOI: 10.1039/C2AN35249D

Both these articles will be free to access for 6 weeks.

Don’t forget to see which HOT articles also feature in this issue.

Follow Analyst on Twitter: @analystrsc

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Advances in Clinical Analysis 2012 Meeting

The Chromatographic Society and the Separation Science Group of the Analytical Division of the Royal Society of Chemistry are pleased to announce their next meeting: Advances in Clinical Analysis 2012

30th October 2012
Robens Suite,
29th Floor,
Guy’s Hospital Tower,
St Thomas’ Street, London, SE1 9RT, UK

Clinical analysis is a wide ranging and complex subject area, but as we move to more complex and difficult analyses, much is now being asked of the techniques in terms of the specificity, sensitivity, simplicity and productivity.

The meeting sets out to look at separation and complementary/alternative techniques along with case studies from the hospital and academic areas. The program has been designed to give attendee’s an update for both routine and problem solving in clinical analysis.

For more information please contact Adrian Clarke.
Tel +41 616963955 e-mail:adrian.clarke@novartis.com

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FACSS welcomes three new member Societies!

The Federation of Analytical Chemistry and Spectroscopy Societies (FACSS) is pleased to announce that at their recent Governing Board meeting in March three new member organizations, namely the AES Electrophoresis Society, the Spectroscopical Society of Japan (SpSJ), and the North American Society for Laser Induced Breakdown Spectroscopy (NASLIBS), have been accepted to the federation as full member organizations.

The Federation of Analytical Chemistry and Spectroscopy Societies (FACSS) is an organization designed to further the interests of analytical chemistry and spectroscopy on behalf of its member organizations.

In 1973, FACSS was founded as a federation of member organizations for the exchange of ideas at the forefront of analytical sciences. Over the last thirty nine years FACSS has pursued five critical objectives to realize the Federation’s goals:

A. Education and Training
B. Advancement of Knowledge
C. Advancement of Technology
D. Social and Professional Peer Networking
E. Promotion / Enabling of Interests of Member Organizations

With the addition of the AES Electrophoresis Society, the Spectroscopical Society of Japan (SpSJ), and the North American Society for Laser Induced Breakdown Spectroscopy (NASLIBS), to the federation’s existing member organizations [Division of Analytical Chemistry of the American Chemical Society, American Society for Mass Spectrometry (ASMS), ANACHEM, the Coblentz Society, the International Society for Automation (ISA) – Analysis Division, the Royal Society of Chemistry (RSC) Analytical Division, and the
Society for Applied Spectroscopy (SAS)], FACSS is now 10-member societies strong. This expansion reflects a unique element of FACSS and its annual North American meeting, SciX, in that all areas and aspects of analytical chemistry and spectroscopy are welcome, bringing together leading scientists from among many disciplines for scientific exchange.

FACSS believes that the addition of these three member organizations will enhance its ability to serve the analytical chemistry and spectroscopy community.

Future North American conferences presented by FACSS will carry the name SciX – the Great Scientific eXchange. Additional information on SciX and the 2012 SciX conference is available at www.scixconference.org.

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HOT articles in Analyst

Take a look below at some wonderful HOT articles we’ve recently published in Analyst. There are papers covering a large variety of fields, including electrochemiluminescence, metabolomics, diabetes research, Raman spectroscopy, and fluorescent probes. These papers will be free to read for two weeks.

Amplified electrochemiluminescence detection of nucleic acids by hairpin probe-based isothermal amplification
Xiaoming Zhou and Da Xing
Analyst, 2012, Advance Article
DOI: 10.1039/C2AN35367A

The surface-enhanced Raman spectra of aflatoxins: spectral analysis, density functional theory calculation, detection and differentiation
Xiaomeng Wu, Simin Gao, Jia-Sheng Wang, Hongyan Wang, Yao-Wen Huang and Yiping Zhao
Analyst, 2012, Advance Article
DOI: 10.1039/C2AN35378D

Eliminating the dication-induced intersample chemical-shift variations for NMR-based biofluid metabonomic analysis
Limiao Jiang, Jing Huang, Yulan Wang and Huiru Tang
Analyst, 2012, Advance Article
DOI: 10.1039/C2AN35392J

Exploratory urinary metabolic biomarkers and pathways using UPLC-Q-TOF-HDMS coupled with pattern recognition approach
Aihua Zhang, Hui Sun, Ying Han, Ye Yuan, Ping Wang, Gaochen Song, Xiaoxia Yuan, Miao Zhang, Ning Xie and Xijun Wang
Analyst, 2012, Advance Article
DOI: 10.1039/C2AN35780A

Use of imaging multivariate analysis to improve biochemical and anatomical discrimination in desorption electrospray ionisation mass spectrometry imaging
Wei Rao, David J. Scurr, James Burston, Morgan R. Alexander and David A. Barrett
Analyst, 2012, 137, 3946-3953
DOI: 10.1039/C2AN35430F

EOT or Kretschmann configuration? Comparative study of the plasmonic modes in gold nanohole arrays
Maxime Couture, Ludovic S. Live, Anuj Dhawan and Jean-Francois Masson
Analyst, 2012, Advance Article
DOI: 10.1039/C2AN35566C

The promise of electrochemical impedance spectroscopy as novel technology for the management of patients with diabetes mellitus
Teagan Leigh Adamson, Francis Ang Eusebio, Curtiss B. Cook and Jeffrey T. LaBelle
Analyst, 2012, Advance Article
DOI: 10.1039/C2AN35645G

Highly selective preconcentration of ultra-trace cadmium by yeast surface engineering
Ting Yang, Xiao-Xing Zhang, Ming-Li Chen and Jian-Hua Wang
Analyst, 2012, Advance Article
DOI: 10.1039/C2AN35755K

Raman microspectroscopy of live cells under autophagy-inducing conditions
Stanislav O. Konorov, Mario A. Jardon, James M. Piret, Michael W. Blades and Robin F. B. Turner
Analyst, 2012, Advance Article
DOI: 10.1039/C2AN35477B

Line shape distortion effects in infrared spectroscopy
Miloš Miljković, Benjamin Bird and Max Diem
Analyst, 2012, 137, 3954-3964
DOI: 10.1039/C2AN35582E

Cd(II)-triggered excimer–monomer conversion of a pyrene derivative: time dependent red-shift of monomer emission with cell staining application
Animesh Sahana, Arnab Banerjee, Sisir Lohar, Subarna Guha, Sudipta Das, Subhra Kanti Mukhopadhyay and Debasis Das
Analyst, 2012, 137, 3910-3913
DOI: 10.1039/C2AN35731C

Protein-templated gold nanoclusters as fluorescence probes for the detection of methotrexate
Zhanguang Chen, Sihua Qian, Xi Chen, Wenhua Gao and Yuejuan Lin
Analyst, 2012, Advance Article
DOI: 10.1039/C2AN35786K

Cavity-enhanced Raman spectroscopy with optical feedback cw diode lasers for gas phase analysis and spectroscopy
Robert Salter, Johnny Chu and Michael Hippler
Analyst, 2012, Advance Article
DOI: 10.1039/C2AN35722D

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HOT Article: A facile graphene oxide-based DNA polymerase assay

Wang et al., Analyst, 2012, 137, 3989

By Laura Ruvuna, Web Writer

In this HOT article, Analyst Advisory Board member Kemin Wang and co-workers from Hunan University, China, use graphene oxide acting as an optical switch in a DNA polymerase assay for the first time. DNA polymerase is important in molecular biological studies and functions as a key drug target. Typically, these assays use expensive and restrictive radioactive labels, which are unsuitable for a high throughput system. Unlike other fluorescence-based assays that require designed template sequences or expensive reagents, here the graphene oxide quenches the fluorescently labeled ssDNA. When the complementary strand binds, the DNA has less of an affinity for the graphene and the resulting fluorescence signal increases. To read more about this new and exciting assay, click the link below. It will be free to read for 2 weeks.

A facile graphene oxide-based DNA polymerase assay
Fengzhou Xu, Hui Shi, Xiaoxiao He, Kemin Wang, Xiaosheng Ye, Lv’an Yan and Shuyong Wei
Analyst, 2012, 137, 3989-3994
DOI: 10.1039/C2AN35585J

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Top ten most accessed articles in June

This month sees the following articles in Analyst that are in the top ten most accessed:-

Easy Dual-Mode Ambient Mass Spectrometry with Venturi Self-Pumping, Canned Air, Disposable Parts and Voltage-Free Sonic-Spray Ionization
Nicolas V. Schwab ,  Andreia M. Porcari ,  Mirela B. Coelho ,  Eduardo M. Schmidt ,  Jose L. Jara ,  Jesui V. Visentainer and Marcos N. Eberlin
Analyst, 2012,137, 2537-2540 DOI: 10.1039/C2AN16312H  

   
A Ratiometric Fluorescent Chemosensor for Iron: Discrimination of Fe2+ and Fe3+ and Living Cell Application 
Supriti Sen ,  Sandipan Sarkar ,  Basab Chattopadhyay ,  Anuradha Moirangthem ,  Anupam Basu ,  Koushik Dhara and Pabitra Chattopadhyay 
Analyst, 2012,137, 3335-3342 DOI: 10.1039/C2AN35258C     

Ultrasensitive Colorimetric Detection of Heparin Based on Self-assembly of Gold Nanoparticles on Graphene Oxide 
Xiuli Fu ,  Lingxin Chen and Jinhua Li  
Analyst, 2012,137, 3653-3658 DOI: 10.1039/C2AN35552C     

A new fluorescent and colorimetric probe for Cu2+ in live cells 
Wei-Yong Liu ,  Hai-Ying Li ,  Bao-Xiang Zhao and Jun-Ying Miao  
Analyst, 2012,137, 3466-3469 DOI: 10.1039/C2AN35559K     

A colorimetric sensor based on catechol-terminated mixed self-assembled monolayers modified gold nanoparticles for ultrasensitive detections of copper ions 
Siqiu Ye ,  Xinhao Shi ,  Wei Gu ,  Yixuan Zhang and Yuezhong Xian 
Analyst, 2012,137, 3365-3371 DOI: 10.1039/C2AN35311C    

Upconversion Nanoparticles in Biological Labeling, Imaging, and Therapy 
Feng Wang ,  Debapriya Banerjee ,  Yongsheng Liu ,  Xueyuan Chen and Xiaogang Liu  
Analyst, 2010,135, 1839-1854 DOI: 10.1039/C0AN00144A     

Bacteriophage based probes for pathogen detection 
Amit Singh ,  Denis Arutyunov ,  Christine M. Szymanski and Stephane Evoy  
Analyst, 2012,137, 3405-3421 DOI: 10.1039/C2AN35371G     

A sensitive dual signal amplification method in western blotting based on antibody-functionalised graphene oxide and gold nanoparticles 
Hongjun Lin ,  Jingrui Huo ,  Aihong Zhang ,  Yingfu Liu ,  Qingming Wang ,  Yun Cai ,  Wantao Ying ,  Weijie Qin ,  Yangjun Zhang and Xiaohong Qian  
Analyst, 2012,137, 3620-3623 DOI: 10.1039/C2AN35242G    

Micro- and nanostructures and their application in gas chromatography 
M. Mittermüller and D. A. Volmer  
Analyst, 2012,137, 3195-3201 DOI: 10.1039/C2AN35184F     

A Fluorescent Probe Directly Detect Peroxynitrite Based on Boronate Oxidation and Its Applications for Fluorescence Imaging in Living Cells 
Fabiao Yu ,  Ping Song ,  Peng Li ,  Bingshuai Wang and Keli Han  
Analyst, 2012,137, 3740-3749 DOI: 10.1039/C2AN35246J     

Why not take a look at the articles today and blog your thoughts and comments below.

Fancy submitting an article to Analyst? Then why not submit to us today or alternatively email us  your suggestions.

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