By Laura Ruvuna, Web Writer
Morgane Moreau and Patrick Schaeffer at James Cook University in Australia have developed a qPCR based binding assay for detecting protein-DNA complexes. Understanding these binding interactions is both biologically vital but difficult to study using traditional methods. Alternatively, this simple procedure requires GFP-Tus, a termination replication protein, a GFP antibody, and multiple strands of DNA in a single reaction. After the immunoprecipitation of the protein-DNA complex, qPCR measures the quantification of each strand separately in polyplex. In addition to being quick and cheap, this assay has the versatility of combining with other common techniques, such as SPR, to increase overall sensitivity. To read more about this group’s new detection method, check out the article below.
A polyplex qPCR-based binding assay for protein–DNA interactions
Morgane J. J. Moreau and Patrick M. Schaeffer
Analyst, 2012, 137, 4111-4113