Archive for January, 2012

Analyst issue 4, 2012 – now online!

The latest issue of Analyst is now available online!

In this issue, the front cover features work by Aaron Timperman and colleagues from West Virginia University, USA, who report the fabrication and performance of a microfluidic traveling-wave electrophoresis system, a promising new separation technique.  It uses a locally defined traveling electric field wave within a microfluidic channel in order to achieve band transport and separation.  The authors anticipate that this technique will open up many future lab-on-a-chip applications, processes and systems.

Fabrication and performance of a microfluidic traveling-wave electrophoresis system, Kyoo D. Jo, Jarrod E. Schiffbauer, Boyd E. Edwards, R. Lloyd Carroll and Aaron T. Timperman, Analyst, 2012, 137, 875-883

The inside front cover of this issue highlights work by Hui-Fen Wu and Kamlesh Shrivas from the National Sun Yat-Sen University, Taiwan, who report the rapid and sensitive protein extraction by cobalt oxide nanoparticle-based liquid-liquid microextraction coupled with MALDI-MS.  The show for the first time that the metal oxide nanoparticles prepared in the organic phase can be successfully applied to the extraction and preconcentration of proteins, and their approach offers distinct advantages including high sensitivity for washing and separation-free MALDI-MS analysis of proteins.

Rapid and highly sensitive protein extraction viacobalt oxide nanoparticle-based liquid–liquid microextraction coupled with MALDI mass spectrometry, Kamlesh Shrivas and Hui-Fen Wu, Analyst, 2012, 137, 890-895

These articles have been made free to access until the 8th March 2012, so why not take a look…

See also the list of Hot articles appearing in this issue which will be free to access for 2 weeks!

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Top ten most accessed articles in December

This month sees the following articles in Analyst that are in the top ten most accessed:-

Stable fluorescent gold nanoparticles for detection of Cu2+ with good sensitivity and selectivity
Yongming Guo, Zhuo Wang, Huawu Shao and Xingyu Jiang 
Analyst, 2012, 137, 301-304 DOI: 10.1039/C1AN15877E  

Colorimetric assay for parallel detection of Cd2+, Ni2+ and Co2+ using peptide-modified gold nanoparticles 
Min Zhang, Yu-Qiang Liu and Bang-Ce Ye 
Analyst, 2012, 137, 601-607 DOI: 10.1039/C1AN15909G 

Modern analytical techniques in metabolomics analysis 
Aihua Zhang, Hui Sun, Ping Wang, Ying Han and Xijun Wang 
Analyst, 2012, 137, 293-300 DOI: 10.1039/C1AN15605E  

Graphene and graphene-based nanomaterials: the promising materials for bright future of electroanalytical chemistry 
Xiao-mei Chen, Geng-huang Wu, Ya-qi Jiang, Yi-ru Wang and Xi Chen 
Analyst, 2011, 136, 4631-4640 DOI: 10.1039/C1AN15661F  

Graphene versus carbon nanotubes for chemical sensor and fuel cell applications 
Douglas R. Kauffman and Alexander Star 
Analyst, 2010, 135, 2790-2797 DOI: 10.1039/C0AN00262C  

Recent advances in electrochemical sensing for hydrogen peroxide: a review 
Wei Chen, Shu Cai, Qiong-Qiong Ren, Wei Wen and Yuan-Di Zhao 
Analyst, 2012, 137, 49-58 DOI: 10.1039/C1AN15738H
  

Advances in microfluidics for environmental analysis 
Jana C. Jokerst, Jason M. Emory and Charles S. Henry 
Analyst, 2012, 137, 24-34 DOI: 10.1039/C1AN15368D  

Hg2+-mediated aggregation of gold nanoparticles for colorimetric screening of biothiols 
Hu Xu, Yiwen Wang, Xiaomei Huang, Yan Li, Hua Zhang and Xinhua Zhong 
Analyst, 2012, 137, 924-931 DOI: 10.1039/C2AN15926K  

Label-free and amplified electrochemical detection of cytokine based on hairpin aptamer and catalytic DNAzyme 
Haixia Zhang, Bingying Jiang, Yun Xiang, Yaqin Chai and Ruo Yuan 
Analyst, 2012, 137, 1020-1023 DOI: 10.1039/C2AN15962G 

Inorganic–organic Ag-rhodamine 6G hybrid nanorods: “Turn on” fluorescent sensors for highly selective detection of Pb2+ ions in aqueous solution 
A. K. Tyagi, Jayshree Ramkumar and O. D. Jayakumar 
Analyst, 2012, 137, 760-764 DOI: 10.1039/C1AN15604G  

Why not take a look at the articles today and blog your thoughts and comments below.

Fancy submitting an article to Analyst? Then why not submit to us today  or alternatively email us your suggestions.

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Hot articles in Issue 4, 2012

The latest issue of Analyst is now online and below are the Hot articles included in this issue.  These articles have all been made free to access until the 13th February 2012, so why not make the most of this and take a look…

And don’t forget to stay informed of all our latest content by signing up to our e-alert or following us on Twitter: @analystrsc

Development of an electrochemical immunosensor for the detection of HbA1c in serum, Guozhen Liu, Sook Mei Khor, Sridhar G. Iyengar and J. Justin Gooding, Analyst, 2012, 137, 829-832     (From the Future Electroanalytical Developments collection)

A microfluidic device for the automated derivatization of free fatty acids to fatty acid methyl esters, Cindy T. Duong and Michael G. Roper, Analyst, 2012, 137, 840-846

Activity monitoring of functional OprM using a biomimetic microfluidic device, Wei Wang, Laura Monlezun, Martin Picard, Philippe Benas, Olivier Français, Isabelle Broutin and Bruno Le Pioufle, Analyst, 2012, 137, 847-852

Electric field standing wave artefacts in FTIR micro-spectroscopy of biological materials, Jacob Filik, Mark D. Frogley, Jacek K. Pijanka, Katia Wehbe and Gianfelice Cinque, Analyst, 2012, 137, 853-861

Recognize three different human telomeric G-quadruplex conformations by quinacrine, Hongxia Sun, Junfeng Xiang, Qian Li, Yan Liu, Lin Li, Qian Shang, Guangzhi Xu and Yalin Tang, Analyst, 2012, 137, 862-867

Resonance Rayleigh scattering spectra of Cu2+–adenine–WO42− system and its analytical application, Qianying Xu, Zhongfang Liu, Xiaoli Hu, Ling Kong and Shaopu Liu, Analyst, 2012, 137, 868-874

Determination of selected natural hormones and endocrine disrupting compounds in domestic wastewater treatment plants by liquid chromatography electrospray ionization tandem mass spectrometry after solid phase extraction, Melis Muz, M. Selcen Sönmez, Okan Tarık Komesli, Sezgin Bakırdere and Celal Ferdi Gökçay, Analyst, 2012, 137, 884-889

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Editorial Board member Duncan Graham to receive Coblentz Society Craver Award

Professor Duncan Graham

Professor Duncan Graham

The Coblentz Society has announced that Professor Duncan Graham, Reviews Editor of Analyst, has been selected as recipient of the 2012 Craver Award.

The Craver Award was established in 2006 to recognize the efforts of young professional spectroscopists that have made significant contributions in applied analytical vibrational spectroscopy. The society has named this award for Clara D. Craver in recognition of her pioneering efforts in promoting the practice of infrared vibrational spectroscopy and her many years of service to the Coblentz Society.  Previous winners of the Award include another Analyst Editorial Board member, Boris Mizaikoff.

This award is presented to Professor Graham in recognition of his pioneering work in surface enhanced Raman scattering (SERS) to generate ultra-sensitive and highly selective methods of detection for a range of analytes, especially bio-analytical targets.

The Craver Award will be presented at the FACSS annual meeting, newly re-named SCIX, to be held in Kansas City, USA, 30 September – 5 October 2012.

Duncan will present the Coblentz Society’s Craver Award Plenary Lecture in Applied Vibrational Spectroscopy and a separate half-day award symposium of six invited presentations will be held following his lecture at this conference.

Take a look at Duncan’s recent Grand Challenges review below, and contact us if you’d like to discuss the submission of a review to his office.

Critical Review: Surface enhanced optical spectroscopies for bioanalysis
Iain A. Larmour and Duncan Graham
Analyst, 2011, 136, 3831-3853
DOI: 10.1039/C1AN15452D

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FACSS Innovation Award Winners

FACSS logoThe Federation of Analytical Chemistry & Spectroscopy Societies (FACSS), of which the RSC is a member society, has announced the winners of its Innovation Awards.

The FACSS Innovation Awards showcase the newest and most creative science debuted orally at a FACSS-organised conference.  At last year’s conference, held in October in Reno, Nevada, USA, the panel selected four equal awardees:

Ultrasound Enhanced ATR mid-IR Fibre Optic Probe for Spectroscopy of Particles in Suspensions
Cosima Koch, Markus Brandstetter, Stefan Radel, Bernhard Lendl
Vienna University of Technology, Austria

Large-Area Standoff Planetary Raman Measurements Using a Novel Spatial Heterodyne Fourier Transform Raman Spectrometer
S. Michael Angel1, Nathaniel R. Gomer1, Shiv K. Sharma2, J. Chance Carter3
1University of South Carolina, 2University of Hawaii, 3Lawrence Livermore National Laboratory

Single Molecule Fluorescence Imaging Studies of Dynamic Processes in Reversed Phase Chromatographic Materials
Justin Cooper, Eric Peterson, Joel Harris
University of Utah

Laser Ablation Molecular Isotopic Spectrometry – New Dimension of LIBS
Alexander Bol’shakov1, Richard Russo1,2, Xianglei Mao2, Dale Perry2, Osman Sorkhabi2, Chris McKay3
1Applied Spectra, Inc., Fremont, CA 94538, 2LBNL, Berkeley, CA 94720, 3NASA-Ames Research Center

Each awardee will be presented with a cash prize and commemorative plaque, and each awardee has been invited to return to the conference in 2012 to become a point of emphasis in the scientific program. The awards will be formally presented at FACSS’s SCIX conference, which will be held in Kansas City, 30 September – 04 October, 2012.

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FACSS Award winners announced

Prof. Peter Griffiths

Professor Peter Griffiths, recipient of the 2012 ANACHEM Award

The Federation of Analytical Chemistry & Spectroscopy Societies (FACSS), of which the RSC is a member society, has announced the winners of two awards.

The 2012 ANACHEM Award will be presented to Peter Griffiths, Emeritus Professor at the University of Idaho, USA.

Professor Griffiths completed his Ph.D. at Oxford University in 1967, and has worked in many different aspects of vibrational spectroscopy since then.  The research in his laboratory has been largely centered on the application of infrared and Raman spectrometry to the solution of problems of analytical, environmental and structural chemistry.

The Association of Analytical Chemists, ANACHEM, is a non-profit organization dedicated to the general welfare and education of analytical chemists and to the advancement of the theory and practice of analytical chemistry as a profession; it was a founding member of FACSS.

Dr Don Pivonka

Dr Don Pivonka, recipient of the 2012 Charles Mann Award

The 2012 Charles Mann Award for Analytical Raman Spectroscopy will be presented to Don Pivonka, a senior principal chemist at  Incyte Pharmaceuticals.

Dr Pivonka received his Ph.D. in analytical chemistry from Kansas State University, and then went on to work for many organisations including ICI and AstraZeneca.  He has been a leader in the use of infrared and Raman spectroscopy within the area of pharmaceutical discovery, and has championed novel applications for understanding relationships between drug candidates and their ligand/receptor interaction in biological systems.

The Charles Mann award for Applied Raman spectroscopy was instituted in 2002 by FACSS following the untimely death of Professor Charles (Charlie) Mann. Professor Mann was a long-standing faculty member at Florida State University, and contributed significantly to the development of analytical Raman spectroscopy.

FACSS logo

SCIX 2012, 30 Sep - 04 Oct, 2012

As award recipients, both Peter and Don will give a plenary lectures at the SCIEX conference in Kansas City later this year.

Congratulations!

You may also be interested in:

Minireview: Non-invasive analysis of turbid samples using deep Raman spectroscopy
Kevin Buckley and Pavel Matousek
Analyst, 2011, 136, 3039-3050
DOI: 10.1039/C0AN00723D

Follow us on Twitter: @analystrsc

He has been a leader in the use of infrared and Raman spectroscopy within the area of pharmaceutical discovery, and has championed novel applications for understanding relationships between drug candidates and their ligand/receptor interaction in biological systems.

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Latest papers in our web theme on Future Electroanalytical Developments

Analyst, 2012, Issue 1Our web theme on Future Electroanalytical Developments continues to grow, and further papers were featured in the most recent issues of Analyst:

Issue 1

Communication: Alkaline phosphatase enzymatic signal amplification for fast, sensitive impedimetric DNA detection
Miriam Kaatz, Holger Schulze, Ilenia Ciani, Fred Lisdat, Andrew R. Mount and Till T. Bachmann; Analyst, 2012, 137, 59-63
DOI: 10.1039/C1AN15767A

A glucose/oxygen enzymatic fuel cell based on redox polymer and enzyme immobilisation at highly-ordered macroporous gold electrodes
Susan Boland and Dónal Leech; Analyst, 2012, 137, 113-117
DOI: 10.1039/C1AN15537G

Electrocatalytic reaction of hydrogen peroxide and NADH based on poly(neutral red) and FAD hybrid film
Kuo Chiang Lin, Yu Ching Lin and Shen Ming Chen; Analyst, 2012, 137, 186-194
DOI: 10.1039/C1AN15739F

Electrochemical sensor for neurotransmitters at physiological pH using a heterocyclic conducting polymer modified electrode
S. Brillians Revin and S. Abraham John; Analyst, 2012, 137, 209-215
DOI: 10.1039/C1AN15746A

Critical Review: Recent advances in electrochemical sensing for hydrogen peroxide
Wei Chen, Shu Cai, Qiong-Qiong Ren, Wei Wen and Yuan-Di Zhao; Analyst, 2012, 137, 49-58
DOI: 10.1039/C1AN15738H

Issue 2Analyst, 2012, Issue 2

Communication: Sensitive and selective voltammetric measurement of Hg2+ by rational covalent functionalization of graphene oxide with cysteamine
Heng Zhou, Xiang Wang, Ping Yu, Xiaoming Chen and Lanqun Mao; Analyst, 2012, 137, 305-308
DOI: 10.1039/C1AN15793K

Study on electrochemical oxidation behaviors and the diffusion mechanism of hydroquinone at pre-anodized carbon paste electrode by cyclic voltammetry
Linjin Ma, Zhenhui Wang and Quanmin Li; Analyst, 2012, 137, 432-436
DOI: 10.1039/C1AN15865A

Resolution of phenolic antioxidant mixtures employing a voltammetric bio-electronic tongue
Xavier Cetó, Francisco Céspedes, Maria Isabel Pividori, Juan Manuel Gutiérrez and Manel del Valle; Analyst, 2012, 137, 349-356
DOI: 10.1039/C1AN15456G

Electropolymerized network of polyamidoamine dendron-coated gold nanoparticles as novel nanostructured electrode surface for biosensor construction
Reynaldo Villalonga, Paula Díez, Santiago Casado, Marcos Eguílaz, Paloma Yáñez-Sedeño and José M. Pingarrón; Analyst, 2012, 137, 342-348
DOI: 10.1039/C1AN15850C

Redox magnetohydrodynamics enhancement of stripping voltammetry of lead(II), cadmium(II) and zinc(II) ions using 1,4-benzoquinone as an alternative pumping species
Ali A. Ensafi, Z. Nazari and I. Fritsch; Analyst, 2012, 137, 424-431
DOI: 10.1039/C1AN15700K

Graphene electroanalysis: Inhibitory effects in the stripping voltammetry of cadmium with surfactant free graphene
Dale A. C. Brownson, Alexandre C. Lacombe, Dimitrios K. Kampouris and Craig E. Banks; Analyst, 2012, 137, 420-423
DOI: 10.1039/C1AN15967D

Issue 3Analyst, 2012, Issue 3

All-solid-state potassium-selective electrode using graphene as the solid contact
Fenghua Li, Junjin Ye, Min Zhou, Shiyu Gan, Qixian Zhang, Dongxue Han and Li Niu; Analyst, 2012, 137, 618-623
DOI: 10.1039/C1AN15705A

A stannum–bismuth composite film electrode for simultaneous determination of zinc(II) and cadmium(II) using differential pulse anodic stripping voltammetry
Nian Bing Li, Wei Wei Zhu, Jun Hua Luo and Hong Qun Luo; Analyst, 2012, 137, 614-617
DOI: 10.1039/C1AN15753A

Transition metal ion-substituted polyoxometalates entrapped in polypyrrole as an electrochemical sensor for hydrogen peroxide
Nargis Anwar, Mikhail Vagin, Fathima Laffir, Gordon Armstrong, Calum Dickinson and Timothy McCormac; Analyst, 2012, 137, 624-630
DOI: 10.1039/C1AN15665A

Towards a detailed in situ characterization of non-stationary electrocatalytic systems
Minghua Huang, John B. Henry, Balázs B. Berkes, Artjom Maljusch, Wolfgang Schuhmann and Alexander S. Bondarenko; Analyst, 2012, 137, 631-640
DOI: 10.1039/C1AN15671C

Non-enzymatic electrochemical detection of glycerol on boron-doped diamond electrode
Aniela Pop, Florica Manea, Ciprian Radovan, Dana Dascalu, Nicolae Vaszilcsin and Joop Schoonman; Analyst, 2012, 137, 641-647
DOI: 10.1039/C2AN15645H

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Top ten most accessed articles in November

This month sees the following articles in Analyst that are in the top ten most accessed:

Stable fluorescent gold nanoparticles for detection of Cu2+ with good sensitivity and selectivity
Yongming Guo, Zhuo Wang, Huawu Shao and Xingyu Jiang
Analyst, 2012, 137, 301-304 DOI:10.1039/c1an15877e

Recent advances and applications in QDs-based sensors
Qiang Ma and Xingguang Su
Analyst, 2011, 136, 4883-4893 DOI:10.1039/c1an15741h

Recent advances in the use of ionic liquids for electrochemical sensing
Debbie S. Silvester
Analyst, 2011, 136, 4871-4882 DOI:10.1039/c1an15699c

Graphene and graphene-based nanomaterials: the promising materials for bright future of electroanalytical chemistry
Xiao-mei Chen, Geng-huang Wu, Ya-qi Jiang, Yi-ru Wang and Xi Chen
Analyst, 2011, 136, 4631-4640 DOI:10.1039/c1an15661f

Sensitive and selective voltammetric measurement of Hg2+ by rational covalent functionalization of graphene oxide with cysteamine
Heng Zhou, Xiang Wang, Ping Yu, Xiaoming Chen and Lanqun Mao
Analyst, 2012, 137, 305-308 DOI:10.1039/c1an15793k

Microbiosensors based on DNA modified single-walled carbon nanotube and Pt black nanocomposites
Jin Shi, Tae-Gon Cha, Jonathan C. Claussen, Alfred R. Diggs, Jong Hyun Choi and D. Marshall Porterfield
Analyst, 2011, 136, 4916-4924 DOI:10.1039/c1an15179g

Electrochemical fabrication of metallic nanostructured electrodes for electroanalytical applications
Blake J. Plowman, Suresh K. Bhargava and Anthony P. O’Mullane
Analyst, 2011, 136, 5107-5119 DOI:10.1039/c1an15657h

Recent advances in electrochemical sensing for hydrogen peroxide: a review
Wei Chen, Shu Cai, Qiong-Qiong Ren, Wei Wen and Yuan-Di Zhao
Analyst, 2012, 137, 49-58 DOI:10.1039/c1an15738h

Graphene electrochemistry: an overview of potential applications
Dale A. C. Brownson and Craig E. Banks
Analyst, 2010, 135, 2768-2778 DOI:10.1039/c0an00590h

A rapid method for detection of genetically modified organisms based on magnetic separation and surface-enhanced Raman scattering
Burcu Guven, İsmail Hakkı Boyacı, Ugur Tamer and Pınar Çalık
Analyst, 2012, 137, 202-208 DOI:10.1039/c1an15629b

Why not take a look at the articles today and leave your thoughts and comments below.

Fancy submitting an article to Analyst? Then why not submit today.

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HOT articles in Issue 3

Analyst, 2012, Issue 3, cover imagesHere at Analyst we like to make it as easy as possible for you to access the latest high-impact analytical science.  So, from now on we’ll let you know which HOT articles feature in each new issue.  Look out for updates, and for longer posts on some of the most exciting new research published.

In Issue 3, you can enjoy the following HOT papers, which will be free to access for 2 weeks:

Minireview: Surface enhanced Raman scattering for multiplexed detection
Jennifer A. Dougan and Karen Faulds
Analyst, 2012, 137, 545-554
DOI: 10.1039/C2AN15979A

Communication: Nonradiative deactivation of europium(III) luminescence as a detection scheme for moisture
Astrid C. Knall, Martin Tscherner, Nadja Noormofidi, Andreas Pein, Robert Saf, Kurt Mereiter, Volker Ribitsch, Franz Stelzer and Christian Slugovc
Analyst, 2012, 137, 563-566
DOI: 10.1039/C1AN15987A

Novel cathodic electrochemiluminescence of tris(bipyridine) ruthenium(II) on a gold electrode in acidic solution
Xiaoquan Lu, Dong liu, Jie Du, Haifeng Wang, Zhonghua Xue, Xiuhui Liu and Xibin Zhou
Analyst, 2012, 137, 588-594
DOI: 10.1039/C1AN15862G

Quantitative analysis of urea in human urine and serum by 1H nuclear magnetic resonance
Lingyan Liu, Huaping Mo, Siwei Wei and Daniel Raftery
Analyst, 2012, 137, 595-600
DOI: 10.1039/C2AN15780B

Colorimetric assay for parallel detection of Cd2+, Ni2+ and Co2+ using peptide-modified gold nanoparticles
Min Zhang, Yu-Qiang Liu and Bang-Ce Ye
Analyst, 2012, 137, 601-607
DOI: 10.1039/C1AN15909G

Hollow mesoporous silica microspheres as sensitive labels for immunoassay of prostate-specific antigen
Dan Wu, Ru Li, Haixia Wang, Shiquan Liu, Huan Wang, Qin Wei and Bin Du
Analyst, 2012, 137, 608-613
DOI: 10.1039/C2AN16033A

A stannum–bismuth composite film electrode for simultaneous determination of zinc(II) and cadmium(II) using differential pulse anodic stripping voltammetry
Nian Bing Li, Wei Wei Zhu, Jun Hua Luo and Hong Qun Luo
Analyst, 2012, 137, 614-617
DOI: 10.1039/C1AN15753A
From a web theme on Future Electroanalytical Developments

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Analyst Issue 3, 2012: now online

Analyst 2012, Issue 3, front cover

Analyst (2012), 137(3): 529-788

Take a look at the latest issue of Analyst, which features three excellent cover articles.

Featured on the front cover is work from Martin Pumera and colleagues of Nanyang Technological University, Singapore.  They studied the performances of several chemically modified graphene nanomaterials as sensing platforms by using the electrochemical impedance spectroscopy technique for the detection and believe that their findings may present a foundation for further research and development in graphene-based impedimetric biosensing.

Detection of DNA hybridization on chemically modified graphene platforms
Marcella Giovanni, Alessandra Bonanni and Martin Pumera
Analyst, 2012, 137, 580-583
DOI: 10.1039/C1AN15910K

The inside front cover features a communication from Andreas Manz of KIST Europe, Germany and his collaborators.  They present a label free quantitative detection method for DNA samples amplified by polymerase chain reaction (PCR) in aqueous medium using terahertz-time domain spectroscopy (THz-TDS)

Analyst 2012, Issue 3, inside front cover

Inside front cover

Communication: Terahertz-time domain spectroscopy for the detection of PCR amplified DNA in aqueous solution
Arun Arora, Trung Quan Luong, Matthias Krüger, Young Jun Kim, Chang-Hoon Nam, Andreas Manz and Martina Havenith
Analyst, 2012, 137, 575-579
DOI: 10.1039/C2AN15820E

Our back cover highlights research from Sangmin Jeon of Pohang University of Science and Technology (POSTECH), Korea, and his co-workers.  They adopted a dynamic light scattering (DLS) method to measure the corrosion of iron nanoparticles.  The nanoparticle corrosion rate determined by DLS was found to be almost identical to the value obtained by conventional immersion tests.

A rapid and facile method for measuring corrosion rates using dynamic light scattering
Jinmyoung Joo, Hyejung Seo, Changho Chun, Kunwoo Han, Hwangyo Jung, Sungjee Kim and Sangmin Jeon
Analyst, 2012, 137, 584-587
DOI: 10.1039/C2AN15975A

All these cover articles will be free to access for 6 weeks so let your colleagues know!

Analyst 2012, Issue 3, back cover

Back cover

This issue also features a number of HOT articles, which will be free for 2 weeks, and 5 papers from our continuing web theme on Future Electroanalytical Developments.

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