Archive for the ‘Hot Article’ Category

HOT articles in Analyst

Take a look at our recent HOT Analyst articles, these are now free to access for the next few weeks!

Designing and fabricating double resonance substrate with metallic nanoparticles–metallic grating coupling system for highly intensified surface-enhanced Raman spectroscopyDesigning and fabricating double resonance substrate with metallic nanoparticles–metallic grating coupling system for highly intensified surface-enhanced Raman spectroscopy
Ying Zhou, Xuanhua Li, Xingang Ren, Liangbao Yang and Jinhuai Liu  
Analyst, 2014, Advance Article
DOI: 10.1039/C4AN00540F

Bimetallic Pd–Pt supported graphene promoted enzymatic redox cycling for ultrasensitive electrochemical quantification of microRNA from cell lysates
Fang-Fang Cheng, Jing-Jing Zhang, Ting-Ting He, Jian-Jun Shi, E. S. Abdel-Halim and Jun-Jie Zhu
Analyst, 2014, Advance Article
DOI: 10.1039/C4AN00777H

Recent developments in proteomic methods and disease biomarkers
Nina Bergman and Jonas Bergquist  
Analyst, 2014, Advance Article
DOI: 10.1039/C4AN00627E

An Ru(II)–Fe(III) bimetallic complex as a multifunctional device for detecting, signal amplifying, and degrading oxalate
Cheuk-Fai Chow, Pui-Yu Ho and Cheng-Bin Gong  
Analyst, 2014, Advance Article
DOI: 10.1039/C4AN00350K

High specific detection of osteopontin using a three-dimensional copolymer layer support based on electrochemical impedance spectroscopy
Hongxia Chen, Qiaohan Mei, Shengsong Jia, Kwangnak Koh, Keming Wang and Xinjian Liu
Analyst, 2014, Advance Article
DOI: 10.1039/C4AN00576G

Fluorometric/colorimetric logic gates based on BODIPY-functionalized mesoporous silica
Heekyoung Choi, Ji Ha Lee and Jong Hwa Jung  
Analyst, 2014, Advance Article
DOI: 10.1039/C4AN00251B

Monitoring UVR induced damage in single cells and isolated nuclei using SR-FTIR microspectroscopy and 3D confocal Raman imaging
Ewelina Lipiec, Keith R. Bambery, Philip Heraud, Wojciech M. Kwiatek, Don McNaughton, Mark J. Tobin, Christian Vogel and Bayden R. Wood  
Analyst, 2014, Advance Article
DOI: 10.1039/C4AN00838C

Up-regulating pyocyanin production by amino acid addition for early electrochemical identification of Pseudomonas aeruginosa
Hunter J. Sismaet, Thaddaeus A. Webster and Edgar D. Goluch  
Analyst, 2014, Advance Article
DOI: 10.1039/C4AN00756E

Analysis of ethyl and methyl centralite vibrational spectra for mapping organic gunshot residues
Jianbo Zeng, Ji Qi, Fuquan Bai, Jorn Chi Chung Yu and Wei-Chuan Shih  
Analyst, 2014, Advance Article
DOI: 10.1039/C4AN00657G

Separation and sensitive determination of sphingolipids at low femtomole level by using HPLC-PIESI-MS/MS
Chengdong Xu, Eduardo Costa Pinto and Daniel W. Armstrong
Analyst, 2014, Advance Article
DOI: 10.1039/C4AN00775A

Affinity-based precipitation via a bivalent peptidic hapten for the purification of monoclonal antibodiesParameters affecting ion intensities in transmission-mode direct analysis in real-time mass spectrometry
Michael W. Handlogten, Jared F. Stefanick, Peter E. Deak and Basar Bilgicer  
Analyst, 2014, Advance Article
DOI: 10.1039/C4AN00780H

Parameters affecting ion intensities in transmission-mode direct analysis in real-time mass spectrometry
Lindsay P. Harding, Gareth M. B. Parkes and James D. Townend  
Analyst, 2014, Advance Article
DOI: 10.1039/C4AN00859F

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Identifying DNA stucture using SERS

Detecting G-quadruplexes using SERS of silver nanoparticles

Differentiating quadruplexes from duplexes using silver nanoparticles and SERS

A promising drug target and ligand-binding site, G-quadruplexes form via Hoogsteen hydrogen bonding in guanine bases. These quartets are rich in guanine and are stabilized through π-π stacking interactions and a cation such as potassium or sodium. As researchers explore new compounds to target this region to treat diseases such as cancer, the structure of these quadruplexes and their response to ligands compared to standard DNA remains understudied. 

Researchers at the University of Strathclyde have used the sensitivity of surface-enhanced Raman spectroscopy (SERS) to detect the formation of G-quadruplexes in the presence of stabilizing ligands and silver nanoparticles. They successfully differentiated the quadruplex DNA structure from the duplex based on the SERS signal, and demonstrated the capability of SERS in identifying higher order DNA structures. To read more about the qualitative detection of these structures, click the link below. It will be free to read until June 30. 

Qualitative SERS analysis of G-quadruplex DNAs using selective stabilizing ligands
K. Gracie, V. Dhamodharan, P. I. Pradeepkumar, K. Faulds and   D. Graham
Analyst
, 2014, Advance Article
DOI: 10.1039/C4AN00551A
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Graphene Hybrids Join the Hunt for Mercury

Graphene-polyfuran hybrids for Hg sensing

Graphene-polyfuran hybrids for Hg sensing

Commonly associated with dental fillings, mercury has a wide range of industrial and domestic applications. However, it can be toxic to humans and the environment even in small concentrations, and has been linked to a number of fatal diseases including pulmonary edema and cyanosis.

Previously developed methods of mercury detection include photochemical, colorimetric and oligonucleotide-based approaches. However, these methods can be slow, expensive and associated with low sensitivity and selectivity for the analyte. Graphene composite materials, with their unusual and enhanced conductivity properties, are an attractive prospect for metal ion analysis.

Researchers led by Professor Jyongsik Jang at the Seoul National University, Korea, have developed a new class of graphene oxide-polyfuran nanotube hybrids. In this “HOT” Analyst paper, the authors test the potential of their graphene-based materials as high performance sensors for mercury ion detection. This method has proved to be highly selective for Hg2+ over other metal ions and has excellent sensitivity even at low mercury concentrations.

This article will be free to read until 30th June.

High-performance Hg2+ FET-type sensors based on reduced graphene oxide–polyfuran nanohybrids
Jin Wook Park, Seon Joo Park, Oh Seok Kwon, Choonghyen Lee and Jyongsik Jang
Analyst, 2014, Advance Article
DOI: 10.1039/C4AN00403E

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HOT articles in Analyst

Take a look at our new HOT articles just published in Analyst and free for you for the next couple of weeks:

Two-dimensional MoS2 nanosheets as a capillary GC stationary phase for highly effective molecular screening
Jia Jia, Fujian Xu, Shanling Wang, Xue Jiang, Zhou Long and Xiandeng Hou 
Analyst, 2014, Advance Article
DOI: 10.1039/C4AN00332B, Communication

On the optimization of operating conditions for Taylor dispersion analysis of mixtures
Hervé Cottet, Jean-Philippe Biron and Michel Martin  
Analyst, 2014, Advance Article
DOI: 10.1039/C4AN00192C, PaperHot articles in Analyst

Capillary electrophoresis based on the nucleic acid detection in the application of cancer diagnosis and therapy
Dong-Sheng Lian and Shu-Jin Zhao  
Analyst, 2014, Advance Article
DOI: 10.1039/C4AN00400K, Minireview

Micropatterning neuronal networks
Heike Hardelauf, Sarah Waide, Julia Sisnaiske, Peter Jacob, Vanessa Hausherr, Nicole Schöbel, Dirk Janasek, Christoph van Thriel and Jonathan West  
Analyst, 2014, Advance Article
DOI: 10.1039/C4AN00608A, Paper

BRET-linked ATP assay with luciferase
Golnaz Borghei and Elizabeth A. H. Hall  
Analyst, 2014, Advance Article
DOI: 10.1039/C4AN00436A, Paper

An ultra-high-throughput spiral microfluidic biochip for the enrichment of circulating tumor cells
Majid Ebrahimi Warkiani, Bee Luan Khoo, Daniel Shao-Weng Tan, Ali Asgar S. Bhagat, Wan-Teck Lim, Yoon Sim Yap, Soo Chin Lee, Ross A. Soo, Jongyoon Han and Chwee Teck Lim  
Analyst, 2014, Advance Article
DOI: 10.1039/C4AN00355A, Paper

A purge and trap integrated microGC platform for chemical identification in aqueous samples
Muhammad Akbar, Shree Narayanan, Michael Restaino and Masoud Agah  
Analyst, 2014, Advance Article
DOI: 10.1039/C4AN00254G, PaperHot articles in Analyst

Hybridization chain reaction-based fluorescence immunoassay using DNA intercalating dye for signal readout
Yan Deng, Ji Nie, Xiao-hui Zhang, Ming-Zhe Zhao, Ying-Lin Zhou and Xin-Xiang Zhang  
Analyst, 2014, Advance Article
DOI: 10.1039/C4AN00190G, Paper

Evaluating the sensitivity of hybridization-based epigenotyping using a methyl binding domain protein
Brandon W. Heimer, Tatyana A. Shatova, Jungkyu K. Lee, Kaja Kaastrup and Hadley D. Sikes  
Analyst, 2014, Advance Article
DOI: 10.1039/C4AN00667D, Communication

Qualitative SERS analysis of G-quadruplex DNAs using selective stabilising ligands
K. Gracie, V. Dhamodharan, P. I. Pradeepkumar, K. Faulds and D. Graham 
Analyst, 2014, Advance Article
DOI: 10.1039/C4AN00551A, Paper

A water-soluble sulfonate-BODIPY based fluorescent probe for selective detection of HOCl/OCl in aqueous media
Jiyoung Kim and Youngmi Kim 
Analyst, 2014,139, 2986-2989
DOI: 10.1039/C4AN00466C, Communication

A coumarin-based fluorescent probe for differential identification of sulfide and sulfite in CTAB micelle solution
Haiyu Tian, Junhong Qian, Qian Sun, Chenjia Jiang, Runsheng Zhang and Weibing Zhang 
Analyst, 2014, Advance Article
DOI: 10.1039/C4AN00478G, Paper

Upconversion nanoparticles for ratiometric fluorescence detection of nitrite
Junfen Han, Cheng Zhang, Fei Liu, Bianhua Liu, Mingyong Han, Wensheng Zou, Liang Yang and Zhongping Zhang 
Analyst, 2014,139, 3032-3038
DOI: 10.1039/C4AN00402G, Paper

Split aptazyme-based catalytic molecular beacons for amplified detection of adenosine
Jin Huang, Yong He, Xiaohai Yang, Kemin Wang, Ke Quan and Xiaoping Lin 
Analyst, 2014,139, 2994-2997
DOI: 10.1039/C4AN00454J, CommunicationHot articles in Analyst

High-performance Hg2+ FET-type sensors based on reduced graphene oxide–polyfuran nanohybrids
Jin Wook Park, Seon Joo Park, Oh Seok Kwon, Choonghyen Lee and Jyongsik Jang 
Analyst, 2014, Advance Article
DOI: 10.1039/C4AN00403E, Communication

One-step prepared fluorescent copper nanoclusters for reversible pH-sensing
Wei Wang, Fei Leng, Lei Zhan, Yong Chang, Xiao Xi Yang, Jing Lan and Cheng Zhi Huang  
Analyst, 2014,139, 2990-2993
DOI: 10.1039/C4AN00113C, Communication

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What Affects the Coffee Ring of Biofluids?

When a drop is applied to a surface and dries, a number of factors control the formation of the dried drop. Essentially as the fluid or solvent evaporates, capillary flow transports molecules to the contact line of the drop with the surface and the drop dries as a ring with components coarsely separated, like a coffee ring.

Images of sessile drop formation

Drop deposition of biofluids is being investigated worldwide as a possible diagnostic tool. However, the parameters that affect the formation of the biofluids coffee ring are not fully understood and this is a barrier that needs to be overcome in order to realise the clinical potential of drop deposited biofluids. Drop deposition is particularly well suited for examining low abundance biofluids such as tears and synovial fluids but is also widely investigated for blood plasma and serum.

A team of US researchers, based at the University of Michigan, have recently published a HOT article characterising biofluids prepared by drop deposition. The researchers studied two model biofluids, blood plasma and synovial fluid, when deposited onto slightly hydrophilic substrates with a contact angle of 50 – 90 degrees. The researchers showed that under most circumstances the model biofluids followed the piling model, as suggested by Deegan et al. and that an increased understanding of the time-dependent rheology and intermolecular forces that occur during evaporation would provide a better approximation. Importantly, from molecular analysis of the drop via Raman spectroscopy, that whilst the morphology of the dried drop changed the chemical composition and molecular structure of the dried proteins within the outer ring were unaffected.

Karen Esmonde-White, one of the authors, comments “The formation of a ring-like structure and compatibility of the drop deposition technique with multiple analytical technologies are well-known features of drop deposition. In this study, we aimed to formalize what is mostly observational data regarding the underlying fluid dynamics of ring formation in drying biofluids. We hope that this work will improve our understanding of the underlying fluid dynamics and their effect on the dried deposit shape and chemical composition. These fundamental studies allow us to define sources of experimental variability in the drop deposition technique and improve its reproducibility. The eventual aim of these studies is clinical translation for examining rheological and chemical changes in synovial fluid associated with joint diseases”.

Characterization of biofluids prepared by sessile drop formation
Karen A. Esmonde-White, Francis W. L. Esmonde-White, Michael D. Morris and Blake J. Roessler
Analyst, 2014,139, 2734-2741
DOI: 10.1039/C3AN02175K
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HOT articles in Analyst

Take a look at our new HOT articles just published in Analyst and free for you for the next couple of weeks: Graphical abstract: Quantum dots in diagnostics and detection: principles and paradigms

Quantum dots in diagnostics and detection: principles and paradigms
T. R. Pisanic II, Y. Zhang and T. H. Wang
Analyst, 2014, Advance Article
DOI: 10.1039/C4AN00294F, Tutorial Review

Cotton fabric-based electrochemical device for lactate measurement in saliva
Radha S. P. Malon, K. Y. Chua, Dedy H. B. Wicaksono and Emma P. Córcoles
Analyst, 2014, Advance Article
DOI: 10.1039/C4AN00201F, Paper

Flow cytometric microsphere-based immunoassay as a novel non-radiometric method for the detection of glutamic acid decarboxylase autoantibodies in type 1 diabetes mellitus
Luciano L. Guerra, Aldana Trabucchi, Natalia I. Faccinetti, Ruben F. Iacono, Daniela B. Ureta, Edgardo Poskus and Silvina N. Valdez
Analyst, 2014, Advance Article
DOI: 10.1039/C3AN02243A, Paper

Enhanced Raman multigas sensing – a novel tool for control and analysis of 13CO2 labeling experiments in environmental research
Robert Keiner, Torsten Frosch, Tara Massad, Susan Trumbore and Jürgen Popp
Analyst, 2014, Advance Article
DOI: 10.1039/C3AN01971C, Paper

Simple sample processing enhances malaria rapid diagnostic test performance
K. M. Davis, L. E. Gibson, F. R. Haselton and D. W. Wright
Analyst, 2014, Advance Article
DOI: 10.1039/C4AN00338A, Paper

To view these article for free, please register for a free RSC account here

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HOT articles in Analyst

Take a look at our new HOT articles just published in Analyst and free for you for the next couple of weeks: Graphical abstract: An electrochemiluminescent microRNA biosensor based on hybridization chain reaction coupled with hemin as the signal enhancer

Fabrication of disposable electrochemical devices using silver ink and office paper
W. R. de Araujo and T. R. L. C. Paixão
Analyst, 2014, Advance Article
DOI: 10.1039/C4AN00097H, Paper

A hybrid paper and microfluidic chip with electrowetting valves and colorimetric detection
Fei He, Jeff Grimes, Samuel D. Alcaine and Sam R. Nugen
Analyst, 2014, Advance Article
DOI: 10.1039/C3AN01516E, Paper

An electrochemiluminescent microRNA biosensor based on hybridization chain reaction coupled with hemin as the signal enhancer
Pu Zhang, Xiaoyan Wu, Yaqin Chai and Ruo Yuan
Analyst, 2014, Advance Article
DOI: 10.1039/C4AN00284A, Paper

Investigating drug induced changes in single, living lymphocytes based on Raman micro-spectroscopy
Iwan W. Schie, Lucas Alber, Amy L. Gryshuk and James W. ChanGraphical abstract: Characterization of biofluids prepared by sessile drop formation
Analyst, 2014, Advance Article
DOI: 10.1039/C4AN00250D, Paper

Single vs. two-photon microscopy for label free intrinsic tissue studies in the UV light region
Vitalijs Zubkovs, Frédéric Jamme, Slavka Kascakova, Franck Chiappini, François Le Naour and Matthieu Réfrégiers
Analyst, 2014, Advance Article
DOI: 10.1039/C4AN00203B, Communication

Characterization of biofluids prepared by sessile drop formation
Karen A. Esmonde-White, Francis W. L. Esmonde-White, Michael D. Morris and Blake J. Roessler
Analyst, 2014, Advance Article
DOI: 10.1039/C3AN02175K, Paper

DNA hybridization detection with 100 zM sensitivity using piezoelectric plate sensors with an improved noise-reduction algorithm
Ceyhun E. Kirimli, Wei-Heng Shih and Wan Y. Shih
Analyst, 2014, Advance Article
DOI: 10.1039/C4AN00215F, Paper

Detection of zeptomole quantities of nonfluorescent molecules in a 101 nm nanochannel by thermal lens microscopy
T. H. H. Le, K. Mawatari, H. Shimizu and T. Kitamori
Analyst, 2014, Advance Article
DOI: 10.1039/C4AN00344F, Paper Graphical abstract: Detection of zeptomole quantities of nonfluorescent molecules in a 101 nm nanochannel by thermal lens microscopy

Microfluidic-SERS devices for one shot limit-of-detection
Donghyuk Kim, Antonio R. Campos, Ashish Datt, Zhe Gao, Matthew Rycenga, Nathan D. Burrows, Nathan G. Greeneltch, Chad A. Mirkin, Catherine J. Murphy, Richard P. Van Duyne and Christy L. Haynes
Analyst, 2014, Advance Article
DOI: 10.1039/C4AN00357H, Paper

Touch spray mass spectrometry for in situ analysis of complex samples
Kevin S. Kerian, Alan K. Jarmusch and R. Graham Cooks
Analyst, 2014, Advance Article
DOI: 10.1039/C4AN00548A, Paper

Criteria for sensitivity enhancement by compressed sensing: practical application to anisotropic NAD 2D-NMR spectroscopy
Krzysztof Kazimierczuk, Olivier Lafon and Philippe Lesot
Analyst, 2014, Advance Article
DOI: 10.1039/C4AN00381K, Paper

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Lights! Camera! ePetri!

Tracking the activity of viruses has never been so easy thanks to the latest development from Chao Han and Changhuei Yang, researchers at the California Institute of Technology, USA.  The duo has developed ePetri, a high performance imaging platform capable of monitoring the formation of viral plaques, an event that occurs when a virus infects a host cell. Unlike conventional plaque assays, ePetri provides high resolution, time-lapse imaging for precise monitoring of the dynamics of viral plaque formation and growth. In addition to enabling better analysis of viral behaviour, the capabilities of ePetri may also facilitate future applications in antiviral drug discovery. Learn more by accessing the link below. 

ePetri Instrument for Viral Plaque Analysis

ePetri Instrument for Viral Plaque Analysis

 

Viral plaque analysis on a wide field-of-view, time-lapse, on-chip imaging platform 

Chao Han and Changhuei Yang 

Analyst, 2014, Advance Article

DOI: 10.1039/C3AN02323K, Paper

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Point-of-Care Paper Based Rapid Allergy Diagnostics

Recent surveys from Allergy UK have shown that the rates of allergy are increasing throughout the world, affecting up to 30-35 % of people at some point during their lives, in some cases with fatal consequences. In vitro testing of specific Immunoglobin E (IgE) reactivities is thought to be able to significantly improve diagnostic accuracy and management in primary care. IgE plays a significant role in allergic conditions. Current methods for the sensitive and accurate detection of specific IgE reactivities are expensive; require significant sample preparation and investment in equipment. There is a need to provide a cheap, rapid, sensitive and specific test for IgE reactivities.

In a recent HOT article, a group of Swedish and Dutch Scientists describe a paper based lateral flow microarray for rapid allergy diagnostics. One of the authors, Jesper Gantelius of the Science for Life Laboratory, highlights “There is a rapid worldwide increase in atopic disorders and asthma without a clear understanding of the underlying cause. We have developed a paper-based microarray assay that could be used to rapidly and cost-efficiently evaluate a large number of specific IgE sensitisation’s in a primary care setting. The device may come to aid doctors in the personalised management of individual patients, as well as allow public health bodies to gain a better insight into the rapid increase of allergy-related conditions.”

The group, led by Prof. Helene Andersson Svahn, describe exciting research that was able to detect the responses of 15 specific IgE responses from 35 clinical serum samples with an inter-assay variability of 12 % revealing good general agreement with a current multiplexed array-based methodology. Interestingly this process is fully scalable and all that is required for data feedback is a consumer-grade flatbed scanner or smartphone camera enabling point-of-care diagnostics.

A lateral flow paper microarray for rapid allergy point of care diagnostics

Thiruppathiraja ChinnasamyLoes I. Segerink, Mats Nystrand, Jesper Gantelius and Helene Andersson Svahn

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HOT articles in Analyst

Take a look at our new HOT articles just published in Analyst and free for you for the next couple of weeks: Graphical abstract: Electrochemiluminescence resonance energy transfer between graphene quantum dots and gold nanoparticles for DNA damage detection

Electrochemiluminescence resonance energy transfer between graphene quantum dots and gold nanoparticles for DNA damage detection
Qian Lu, Wei Wei, Zhenxian Zhou, Zhixin Zhou, Yuanjian Zhang and Songqin Liu
Analyst, 2014, Advance Article
DOI: 10.1039/C4AN00020J, Paper

A lateral flow paper microarray for rapid allergy point of care diagnostics
Thiruppathiraja Chinnasamy, Loes I. Segerink, Mats Nystrand, Jesper Gantelius and Helene Andersson Svahn
Analyst, 2014, Advance Article
DOI: 10.1039/C3AN01806G, Paper

Nanoparticles in sensing applications: on what timescale do analyte species adsorb on the particle surface?
Enno Kätelhön and Richard G. Compton
Analyst, 2014, Advance Article
DOI: 10.1039/C4AN00363B, Paper

The use of EEM fluorescence data and OPLS/UPLS-DA algorithm to discriminate between normal and cancer cell lines: a feasibility study
Ana Carolina de Oliveira Neves, Raimundo Fernandes de Araújo Júnior, Ana Luiza Cabral de Sá Leitão Oliveira, Aurigena Antunes de Araújo and Kássio Michell Gomes de Lima Graphical abstract: A lateral flow paper microarray for rapid allergy point of care diagnostics
Analyst, 2014, Advance Article
DOI: 10.1039/C4AN00296B, Paper

Enhancing permanganate chemiluminescence detection for the determination of glutathione and glutathione disulfide in biological matrices
Zoe M. Smith, Jessica M. Terry, Neil W. Barnett, Laura J. Gray, Dean J. Wright and Paul S. Francis
Analyst, 2014, Advance Article
DOI: 10.1039/C4AN00255E, Paper

Mass spectrometric investigation of the role of the linking polypeptide chain in DNA polymerase I
Taeho Yeom, Jungyoon Lee, Seonghyun Lee, Sunah Kang, Kyung Rok Kim, Byungwoo Han, Hyun Soo Lee and Kyubong Jo
Analyst, 2014, Advance Article
DOI: 10.1039/C4AN00107A, Paper

Optimizing detection of RDX vapors using designed experiments for remote sensing
Robert G. Ewing, Alejandro Heredia-Langner and Marvin G. WarnerGraphical abstract: Nanoparticles in sensing applications: on what timescale do analyte species adsorb on the particle surface?
Analyst, 2014, Advance Article
DOI: 10.1039/C4AN00125G, Paper

Mixed hydrogel bead-based tumor spheroid formation and anticancer drug testing
Yaolei Wang and Jinyi Wang
Analyst, 2014, Advance Article
DOI: 10.1039/C4AN00015C, Paper

Carbon nanobelts as a novel sensing platform for fluorescence-enhanced DNA detection
Xuping Sun, Zhicai Xing, Rui Ning, Abdullah M. Asiri and Abdullah Y. Obaid
Analyst, 2014, Advance Article
DOI: 10.1039/C3AN02364H, Communication

To view these article for free, please register for a free RSC account here

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