Jeonggi Seo and Meng H. Lean
Palo Alto Research Center, Palo Alto, CA, USA
Why is this useful?
Syringes are commonly used to supply or collect liquid samples for microfluidics. However, interfacing between a microfluidic chip and syringe is problematic due to their size difference. Plastic tube connectors or Luer stubs have been used as interfacing methods, but they increase sample dead volume and thus experimental cost. The goal of this tip is to provide an inexpensive and simple interface, with minimized sample dead volume, between a microfluidic device and syringe.
Figure 1.
What do I need?
- 18 gauge blunt needle
- PTFE microbore tubing (0.012″ID x 0.030″OD, Cole Parmer)
- Silicone (platinum-cured) tubing (1/50″ID x 1/12″OD, Cole Parmer)
- B-D Disposable Syringes 1 mL
What do I do?
1.Punch access holes into your PDMS device with an 18 gauge blunt needle.
2.Cut a segment of silicone tubing ~10 mm long. (Figure 1)
3.Cut a segment of PTFE microbore tubing with desirable length for your set-up.
Figure 2.
4.Insert the piece of silicone tubing inside the 1 ml syringe tip. Make sure the silicone tubing reaches the inner chamber of the syringe but doesn’t disturb piston movement. (Figure 2a-c)
5.Insert the PTFE tubing into the inserted silicone tubing. (Figure 2d)
6. Load your sample into the syringe
7. Insert the free end of the PTFE tubing into the access hole on your PDMS device (Figure 3). Since no adhesive is involved, the PTFE tubing can be disposed of or reused after cleaning.
References
S. Mohanty, G. Mensing and D. J. Beebe, PDMS connectors for macro to microfluidic interfacing, Chips & Tips (Lab on a Chip), 23 October 2006.
Movers packers In Gurgon do this execute very smartly and quickly to shift the house. The packers and movers in Bangalore handle the whole procedure as per the requirements of the consumer or according to need of client.
Expert 5 Packers and Movers Delhi go to
http://www.expert5th.in/packers-and-movers-delhi/
Expert 5 Packers and Movers Gurgaon go to
http://www.expert5th.in/packers-and-movers-gurgaon/
Expert 5 Packers and Movers Chennai go to
http://www.expert5th.in/packers-and-movers-chennai/
Top 6 Packers and Movers Pune go to
http://packersmoverspune.top3rd.in/
Expert 5 Packers and Movers Hyderabad go to
http://www.expert5th.in/packers-and-movers-hyderabad/
Expert 5 Packers and Movers Bangalore go to
http://www.expert5th.in/packers-and-movers-bangalore/
Expert 5 Packers and Movers Mumbai go to
http://www.expert5th.in/packers-and-movers-mumbai/
They offer his or her solutions not merely associated with home new home buy also for company shifting,
Packers and Movers in Hyderabad @ http://www.expert5th.in/packers-and-movers-hyderabad/
Packers and Movers in Mumbai @ http://www.expert5th.in/packers-and-movers-mumbai/
Packers and Movers in Pune @ http://packersmoverspune.top3rd.in/
Nevertheless there are many people who issue going packers moreover to movers being an outstanding luxury
Packers and Movers in Bangalore @ http://www.expert5th.in/packers-and-movers-bangalore/
Packers and Movers in Delhi @ http://www.expert5th.in/packers-and-movers-delhi/
Packers and Movers Gurgaon @ http://www.expert5th.in/packers-and-movers-gurgaon/
Create sure that a efficient movers provides a personal hassle-free as well as at convenience connection with breaking.
Packers and Movers Chennai @ http://www.expert5th.in/packers-and-movers-chennai/
Packers and Movers Noida @ http://www.expert5th.in/packers-and-movers-noida/
The harder top high quality such as assistance is usually, far more price you should pay.
Packers and Movers Chennai @ http://www.expert5th.in/packers-and-movers-chennai/
Packers and Movers Noida @ http://www.expert5th.in/packers-and-movers-noida/
Probably the most trial concerning going will be determining what you should acquire or even what you should aside.
Packers and Movers in Hyderabad @ http://www.expert5th.in/packers-and-movers-hyderabad/
Packers and Movers in Mumbai @ http://www.expert5th.in/packers-and-movers-mumbai/
Prepare acceptable items assistance such as vehicle associated with transport regarding aspects.
Movers and Packers in Mumbai
Movers and Packers in Hyderabad
This can certainly not assistance you to cut down going costs and also allow you to generate some sources.
Packers and Movers in Pune
Packers and Movers in Bangalore
Go along with movers providing an personal warehousing & safe-keeping assistance to your items without price or even a little price.
Packers and Movers in Pune
Packers and Movers in Bangalore
Thanks for sharing your info. I really appreciate your efforts and I will be waiting for your further write
Packers and Movers in Hyderabad
Packers and Movers in Bangalore
Packers and Movers in Mumbai
Thanks for sharing. I hope it will be helpful for too many people that are searching for this topic-
Packers and Movers in Chennai
Packers and Movers in Pune
Packers and Movers in Delhi
Packers and Movers in Gurgaon