Click’n lock: rapid exchange between unsymmetric tetrazines and thiols for reversible, chemoselective functionalisation of biomolecules with on-demand bioorthogonal locking

About this article:

Click reactions play a crucial role in efficiently modifying complex biomolecules, particularly in the realm of biotherapeutics. The continuous challenge lies in their reversible and irreversible transformations, with chemists seeking ultimate control over molecular structures in dilute conditions and crowded environments.

In this groundbreaking article, we introduce the Click’n Lock principle, describing a novel reaction system capable of seamlessly switching from reversible to irreversible click transformations. Termed ‘TeTEx’ for ‘tetrazine – thiol exchange,’ this concept enables on-demand locking of products using bioorthogonal stimuli (dienophiles), providing a transformative switch from reversible to irreversible attachment.




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RSC Chemical Biology

Royal Society of Chemistry