Prof. Amar Basu from Wayne State University, USA, uses a tutorial video to explain his new freely available software tool Droplet Morphometry and Velocimetry (DMV):
A common problem for researchers in droplet microfluidics is the quantitative analysis of droplets. Developments in the droplet microfluidics literature have shown that there is a great deal of relevant information encoded into a droplet’s physical characteristics, such its size, shape, velocity and trajectory. Indeed, several techniques utilize such characteristics as measurements for label-free assays. Currently however, much of the video or image analysis must be done manually.
Droplet Morphometry and Velocimetry (DMV) is a software tool which uses image processing techniques to track droplets in digital videos, providing quantitative, time-resolved, label-free measurements. DMV tracks 20 different parameters, including size, shape, trajectory, velocity, pixel statistics and nearest neighbor spacing. Our recent Lab on a Chip paper provides details on DMV performance and how it can be used to analyze common droplet operations and systems reported by industry and academic labs, including: droplet generators, splitting and merging devices, cell encapsulation efficiency, serial dilutions, emulsion packing, size distributions and sorting efficiency.
Droplet Morphometry and Velocimetry (DMV): A video processing software for time-resolved, label-free tracking of droplet parameters
A. S. Basu
DOI:10.1039/C3LC50074H
DMV is available free of charge to researchers in the droplet microfluidics community. Currently >25 labs worldwide have requested the software. To obtain the software, please feel free to contact Prof. Amar Basu at abasu@eng.wayne.edu. Accompanying the software are a video training tutorial, installation tutorial and a playlist of videos showing the application of DMV in various operations.
This example video of DMV applied to drop splitting is one of eight available application example videos:
We welcome your feedback and new applications of DMV! Updates will be posted to our website at http://microfluidics.wayne.edu.
DMV video tutorial (58 minutes, HD): http://www.youtube.com/watch?v=XOaYX4Zk9mA
DMV Installation tutorial (4 minutes, HD): http://www.youtube.com/watch?v=lDpAIn2MsAQ
