A cheap PDMS biochip has been developed by Roland Thuenauer and Alois Sonnleitner, Center for Advanced Bioanalysis, Austria, that enables the recording of individual vesicle fusion events at the apical membrane of live cells.
The cells can be grown to polarize directly on the device and then correctly placed by an integrated micro-positioning system in order to perform apical TIRF microscopy, without the need for an additional weight to force the apical membrane of the cells into the region of the evanescent wave.
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A PDMS-based biochip with integrated sub-micrometre position control for TIRF microscopy of the apical cell membrane
Roland Thuenauer, Kata Juhasz, Reinhard Mayr, Thomas Frühwirth, Anna-Maria Lipp, Zsolt Balogi and Alois Sonnleitner