HOT article: Testing drug metabolism

Permentier et al., Analyst, 2012, Advance Article

By Jennifer A. Dougan, Imperial College London, Web Writer

Testing drug metabolism by in vitro electrochemistry as a mimic for the in vivo action of enzymes is an established technique in drug metabolism and toxicological studies. These investigations are an important part of the drug discovery and development process.  Using lidocaine as a model substrate to test the applicability of their new electrochemical techniques, Hjalmar P. Permentier‘s group from the Analytical Biochemistry and Mass Spectrometry Core Facility at the University of Groningen have successfully expanded their electrochemical toolbox to more closely mimic the action of Cytochrome P450s.  Using a Pt electrode and electrocatalytic oxidation of H2O2, 3-hydroxylation of lidocaine has been observed, thereby extending the scope and validity of in vitro electrochemical-imitation of Cytochrome P450 enzymatic action.

Electrocatalytic oxidation of hydrogen peroxide on a platinum electrode in the imitation of oxidative drug metabolism of lidocaine
Eslam Nouri-Nigjeh, Andries P. Bruins, Rainer Bischoff and Hjalmar P. Permentier
Analyst, 2012, Advance Article
DOI: 10.1039/C2AN35388A

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