HOT Article: Using FTIR to diagnose cervical cancer

Correlation of p16INK4A expression and HPV copy number with cellular FTIR spectroscopic signatures of cervical cancer cells In this HOT Article a team from Dublin present an impressive study that links imunohistochemistry and fluorescence with FTIR to demonstrate the effectiveness of spectroscopic techniques in the diagnosis of cervical cancer.

Fluorescence microscopy and flow cytometry are used to deomonstrate that expression levels of the biomarker protein p16INK4A in cervical cancer cell lines correlates with HPV invection levels.  This confirms p16INK4A as a potential diagnostic marker of cervical cancer. FTIR imaging is then used to identify the specific spectral features of nuclear and cytoplasmic regions of the cervical cancer cells.  By correlating all the findings it was possible to construct a model which can predict the p16INK4A expression level based on a spectral fingerprint of a cell line, demonstrating the diagnostic potential of spectroscopic techniques.

Interested in knowing more?  Read the article here, free until March 11th.

Correlation of p16INK4A expression and HPV copy number with cellular FTIR spectroscopic signatures of cervical cancer cells
Kamila M. Ostrowska, Amaya Garcia, Aidan D. Meade, Alison Malkin, Ifeoluwapo Okewumi, John J. O’Leary, Cara Martin, Hugh J. Byrne and Fiona M. Lyng
Analyst, 2011, Advance Article
DOI: 10.1039/C0AN00910E

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3 Responses to “HOT Article: Using FTIR to diagnose cervical cancer”

  1. [...] This post was mentioned on Twitter by apotwi, Molecular Probes. Molecular Probes said: HOT Article: Using FTIR to diagnose cervical cancer: http://ow.ly/3ULFY [...]

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  2. Faisal says:

    This lethal disease needs awareness as it can be treated if it is detected early. Use of condoms can reduce but does not always prohibit the contraction of this disease. Thailand Cervical cancer hospital

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  3. FTIR says:

    Indeed a wonderful post. I have been studying the chromatography, spectroscopy etc. and do look over the web for latest and informative stuff and just landed on your blog. Thanks for writing such good posts and as I have subscribed to your blog, I do expect that you will be posting nice stuff like this on a regular basis.

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