Filming the motions of individual lipids

Thomas Just Sørensen is a guest web-writer for PCCP. He is currently a post-doctoral researcher at the University of Copenhagen, Denmark.

Image of observation area, lipid bilayer and cover glassSuper resolution microscopy breaks the diffraction limit. Professor Dr Stefan Hell is credited for the development of STED or stimulated emission depletion microscopy. In this paper arising from the Faraday Discussion on Lipids & Membrane Biophysics STED is used in combination with FCS or fluorescence correlation spectroscopy to follow the diffusion of dye labeled lipids in membrane model systems on glass and mica.

Super resolution microscopy is used to study the diffusion of labelled lipids, two labels are employed, one that localises in the liquid ordered and one that localises in the liquid disordered phase of the lipid membrane model. Through STED, and with the two new probes introduced, the rate of 2D diffusion in the membrane can be measured. As the localisation of the two probes is different, a map of the disordered and ordered domains can be generated.

by Dr Thomas Just Sørensen

Read this fascinating article which was presented as part of the Faraday Discussion on Lipids & Membrane Biophysics:

STED microscopy detects and quantifies liquid phase separation in lipid membranes using a new far-red emitting fluorescent phosphoglycerolipid analogue
Alf Honigmann, Veronika Mueller, Stefan W. Hell and Christian Eggeling
DOI: 10.1039/C2FD20107K

Read all the results and Discussion in the Lipids & Membrane Biophysics Discussion volume.

We’d love you to join us at a future meeting: more details on the Faraday Discussions events website.

Digg This
Reddit This
Stumble Now!
Share on Facebook
Bookmark this on Delicious
Share on LinkedIn
Bookmark this on Technorati
Post on Twitter
Google Buzz (aka. Google Reader)

Leave a Reply