In this Catalysis Science & Technology Hot article Nigel S. Scrutton and coworkers from the University of Manchester look at pentaerythritol tetranitrate reductase (PETN reductase) and how site-directed mutagenesis can effect its biocatalytic action.
Scrutton uses PETN, which is a member of the Old Yellow Enzyme family of enzymes. Old Yellow Enzymes were first isolated from brewers’ bottom yeast back in 1932, and they have been extensively studied ever since.
Read the full article to find out more about the role of key active sites in PETN reductase…
Active site modifications in pentaerythritol tetranitrate reductase can lead to improved product enantiopurity, decreased by-product formation and altered stereochemical outcome in reactions with α,β-unsaturated nitroolefins
Anna Fryszkowska, Helen Toogood, Michiyo Sakuma, Gill M. Stephens, John M. Gardiner and Nigel S. Scrutton
Catal. Sci. Technol., 2011, Advance Article
DOI: 10.1039/C0CY00092B, Paper
FREE TO READ!
