Poul Nielsen and his group at University of Southern Denmark describe the synthesis of new double-headed nucleosides, using the CuAAC reaction, and their influence on the stability of the hybridization of oligonucleotides.
In this HOT paper they describe a new type of nucleosides that are able to stabilize three-way junctions. If you want to find out more about these interesting stabilising contacts download the article, which is free to access until the 8th February.
The synthesis of double-headed nucleosides by the CuAAC reaction and their effect in secondary nucleic acid structures
Anna S. Jørgensen, Khalil I. Shaikh, Gerald Enderlin, Elise Ivarsen, Surender Kumar and Poul Nielsen
Org. Biomol. Chem., 2011, Advance Article
To give you a better overview on Nielsen’s previous research, we have outlined below a collection of their latest OBC published papers. Enjoy!
Stabilisation of nucleic acid secondary structures by oligonucleotides with an additional nucleobase; synthesis and incorporation of 2′-deoxy-2′-C-(2-(thymine-1-yl)ethyl)uridine
Søren Ljungberg Pedersen and Poul Nielsen
Org. Biomol. Chem., 2005, 3, 3570-3575
Synthesis and modelling of DNA junction and minor groove zipper motifs incorporating the double-headed nucleoside 5′(S)–C-(thymine-1-ylmethyl)thymidine
Mikkel S. Christensen, Charlotte M. Madsen and Poul Nielsen
Org. Biomol. Chem., 2007, 5, 1586-1594
Nucleic acid secondary structures containing the double-headed nucleoside 5′(S)-C-(2-(thymin-1-yl)ethyl)thymidine
Charlotte Andersen, Pawan K. Sharma, Mikkel S. Christensen, Signe I. Steffansen, Charlotte M. Madsen and Poul Nielsen
Org. Biomol. Chem., 2008, 6, 3983-3988