Spectroscopic methods can be used to evaluate distribution of metals in the mitochondria of yeast cells.
Paul Lindahl and colleagues at the Texas A&M University, USA, investigated the function of the Mtm1p, a carrier protein found in the yeast Saccharomyces cervisiae. Deleting the Mtm1p gene, they evaluated the iron and manganese presence in the cells and discovered that iron and manganese accumulate in mitochondria and inactivate the enzyme Mn-superoxide dismutase. Metal concentrations in cells and mitochondria were efficiently measured using inductively coupled plasma mass spectrometry.
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Insights into the iron-ome and manganese-ome of Δmtm1 Saccharomyces cerevisiae mitochondria Jinkyu
Park, Sean P. McCormick, Mrinmoy Chakrabarti and Paul A. Lindahl
Metallomics, 2013, Advance Article