This research is from Michael W. Linscheid group from Humboldt-Universitaet zu Berlin, Department of Chemistry, Germany in collaboration with Jurgen Thomale, University of Duisburg-Essen, Institute of Cell Biology, Germany. The authors present a methodology to measure the complex adduct spectrum caused by the interaction of Cisplatin with DNA using HPLC/ESI-MS and HPLC/ICP-MS. The research shows that the adduct spectrum in in vivo duplex DNA is much more complex than described so far in the literature and after the initial formation of adducts a rearrangement occurs on the DNA-strands leading to significant changes in adduct patterns over time. This methodology provides new options to characterize and understand the biological consequences of the wide variety of detected Cisplatin–DNA adducts. This paper will be free to read until Oct 22nd.
On the complexity and dynamics of in vivo Cisplatin–DNA adduct formation using HPLC/ICP-MS
Matthias Ziehe, Diego Esteban-Fernández, Ulrike Hochkirch, Jürgen Thomale and Michael W. Linscheid
Metallomics, 2012, 4, 1098-1104