Starting the week with a HOT article: sorting droplets by number of encapsulated particles

droplet sortingDroplet microfluidics has recently been used for encapsulating a number of different objects, including cells and microbeads. This enables analysis, detection and screening of the encapsulated material. The control of encapsulation to get droplets containing an equal number of particles is important, as often a researcher may need singly occupied droplets, but some of the resulting droplets may be empty or have one or more particles inside with current techniques.

Sorting by particle number would therefore be very helpful for isolating the needed droplets. For larger droplets, sorting by size doesn’t work as they remain the same size whether empty or encapsulating a particle. Fluorescence intensity is also often used as the basis for droplet sorting. However no technique currently sorts by particle number alone.

In this HOT article, collaborators in the USA and China led by Chang Lu develop a one-layer microfluidic device that sorts droplets based on number of particles.  It enables production of droplets with even number of encapsulated particles and the analysis of droplets with mixed particle numbers. It can sort up to 30 droplets a second very specifically. The droplets flow through a narrow channel and in this they are detected one-by-one by laser-induced fluorescence. A microcontroller uses this signal to make the sorting decision and a solenoid valve deflects the droplets into either waste or the collection channel. The microcontroller makes the decision based on the time interval between two travelling particles – if there are two particles in one droplet, the interval is smaller than the time taken for the droplet to pass through. It also counts the number of particles in one droplet.

This article free to access for the next 4 weeks* as with all of our HOT articles, so you can have a read and closer look at the technical diagrams by clicking on the link below:

Droplet sorting based on the number of encapsulated particles using a solenoid valve
Zhenning Cao, Fangyuan Chen, Ning Bao, Huacheng He, Peisheng Xu, Saikat Jana, Sunghwan Jung, Hongzhen Lian and Chang Lu
DOI: 10.1039/C2LC40950J

 *Free access to individuals is provided through an RSC Publishing personal account. Registration is quick, free and simple

Digg This
Reddit This
Stumble Now!
Share on Facebook
Bookmark this on Delicious
Share on LinkedIn
Bookmark this on Technorati
Post on Twitter
Google Buzz (aka. Google Reader)

10 Responses to “Starting the week with a HOT article: sorting droplets by number of encapsulated particles”

  1. Leola Zamora says:

    that would contain k particles. From Figure 3, both the single and double particle encapsulation efficiencies outperform random encapsulation efficiencies by over a factor of two and greatly reduce the number of drops with more than the desired number of particles. Figure 4 demonstrates the need for proper concentrations for high efficiency; that is, λ, a function of both particle concentration and drop volume, should be equal or close to the number of desired cells per drop to maximize correctly-encapsulated particles or cells. Note that a higher concentration of particles or cells is usually a good thing for full ordering as dense trains tend to spread out over time and fill emptier regions between trains. On the other hand, if concentration is too high, the high number of particles may cause interfacial instabilities that induce jetting at the nozzle. In specific studies (such as single-cell encapsulation, for example), it may be more advantageous to avoid multiple-cell droplets at the expense of introducing a few more empty droplets, so a slightly lower λ will be desired. This would also apply for studies aimed at interactions between two cells or between a cell and a particle, where single-particle or single-cell droplets are more tolerable than droplets with two or more of one kind of cell or particle.

  2. Put equivalent objects together. This particular can help you throughout unpacking. Eradicate worthless kitchen area objects or maybe foodstuffs. Don’t bring these people along with you.
    Packers and Movers Faridabad
    Movers and Packers in Faridabad
    Packers and Movers in Faridabad
    Movers and Packers Faridabad
    Packers and Movers Noida
    Packers and Movers Ghaziadabad

  3. Most are trying to find very best assistance generate or even very best item. With Transferring industry also they are seeking very best packers as well as movers inside of Chennai.
    Movers and Packers in Chennai
    Movers and Packers in Hyderabad
    Movers and Packers in Bangalore
    Movers and Packers in Delhi

  4. that concept caused by products youngster should be assures in addition to enables considering ample basically considering efficient amazing may gain in addition to purchases basically word-of-mouth other than claims in addition to dealings in addition to statements as well as statements and affirmations set
    Packers and Movers Navi Mumbai
    Packers and Movers in Navi Mumbai
    Movers and Packers Navi Mumbai
    Movers and Packers in Navi Mumbai
    wishes profits prior to improve. Women and men might actually typically upon that will schedule precisely what specifically substantially hereafter impressive greatest inside the retailers related to at this time definitive usually.
    http://top3th.co.in/packers-and-movers-thane/

  5. Packers in addition to Movers usually are the very best choice to own most suited in addition to trusted relocating in addition to trucking businesses.

    Great 5 Packers and Movers in Pune

    Great 5 Packers and Movers in Bangalore

    Great 5 Packers and Movers in Gurgaon

  6. Stick to in which level which could be restorative healing for people. Although producing your current moving over and also moving by using

    Great 5 Packers and Movers in Mumbai

    Great 5 Packers and Movers in Delhi

    Great 5 Packers and Movers in Noida

  7. Local packers together with movers in the course of Faridabad allows an abundance of people, members of the family together with practices move to a different advanced, neighborhood, town together with town.

    Packers and Movers Gurgaon
    Packers and Movers Pune
    Packers and Movers Mumbai
    Packers and Movers Hyderabad
    Packers and Movers Bangalore
    Packers and Movers Chennai
    Packers and Movers Delhi

  8. SEO EXPERT says:

    Owning some preferred photographs and desire
    to widespread them, then fail to be concerned as
    being an amazing method is patiently waiting that
    is definitely how to get 9k followers on instagram that you can allow you to be well known as well as your images well-known in a outstanding and easy approach.

  9. Thanks for information Export5th company top services for packing and moving services in India
    Packers and Movers in Gurgaon
    Packers and Movers in Pune
    Packers and Movers in Mumbai

Leave a Reply

*