Microarrays offer the opportunity for the high-throughput study of protein-protein interactions that may uncover drug targets and provide early detection of various diseases. Most current technologies rely on fluorescent labelling of proteins, but this is costly, time-consuming and can be inaccurate due to steric interference, quenching and photo-bleaching.
Hatice Altug, Boston University, and colleagues from MIT have now found a way to accomplish label free detection by using a dual-colour filter method to image large scale plasmonic nanohole arrays. Their high-density detection platform has over 1 million sensors and has reliably demonstrated massively multiplexed detection of antibody bindings with reduced image acquisition time.
Download the paper to read how they did it, it’s free to access for the next 4 weeks:
Large-scale plasmonic microarrays for label-free high-throughput screening
Tsung-Yao Chang, Min Huang, Ahmet Ali Yanik, Hsin-Yu Tsai, Peng Shi, Serap Aksu, Mehmet Fatih Yanik and Hatice Altug
Lab Chip, 2011, Advance Article