This month’s outside front cover features work from Jonathan Rocheleau and co-workers at the University of Toronto.
In their article, they introduce a novel method for flavoprotein autofluorescence imaging, to measure the dynamics of fatty acid oxidation linked to pancreatic islet metabolism. They were able to record real-time measurements from islets held stationary in flow using microfluidic devices.
Quantitative imaging of electron transfer flavoprotein autofluorescence reveals the dynamics of lipid partitioning in living pancreatic islets
Alan K. Lam, Pamuditha N. Silva, Svetlana M. Altamentova and Jonathan V. Rocheleau
Work from Konstantinos Konstantopoulos, Kathleen Stebe and colleagues from The Johns Hopkins University and the University of Pennsylvania, was the inspiration for the inside front cover this month.
The article focuses on receptor-mediated cell adhesion in shear flow, and uses an integrated experimental and mathematical approach to gain understanding of the process. Using a microfluidic device combined with a glass slide with P- or L-selectin immobilised onto specific sections, the critical patch length needed by HL-60 leukemic cells to start tethering under flow was investigated. The authors found that the length is prescribed by the minimum number of receptor–ligand bonds needed to start cell tethering and the tensile strength of the bonds.
Selectin-mediated adhesion in shear flow using micropatterned substrates: multiple-bond interactions govern the critical length for cell binding
ZiQiu Tong, Luthur Siu-Lun Cheung, Kathleen J. Stebe and Konstantinos Konstantopoulos
Lance Munn and collaborators at Massachusetts General Hospital and Harvard Medical School’s article features on the back cover of issue 8.
Their paper focuses on vascular anastomosis, and uses a microfluidic device to reproduce the process in vitro. Using a collagenous matrix, the vessels are able to sprout and grow together, forming perfused bridging connections. The authors feel that the device will ‘enable a new generation of studies of the mechanisms of angiogenesis and provide a novel and practical platform for drug screening’.
Anastomosis of endothelial sprouts forms new vessels in a tissue analogue of angiogenesis
Jonathan W. Song, Despina Bazou and Lance L. Munn
Also included in the issue is a review on the role of regucalcin in brain calcium signalling and papers focussing on a method to calculate the change of network metabolites for different time points of transcriptomic datasets and the differential effects of a soluble or immobilized VEGFR-binding peptide.