The front cover of this month’s issue highlights work by Valerică Raicu et al. on determining the dynamic structure of an important transmembrane transporter protein complex using FRET. This interesting HOT article is also the subject of the previous IBiology blog post.
Determination of the quaternary structure of a bacterial ATP-binding cassette (ABC) transporter in living cells
Deo R. Singh, Mohammad M. Mohammad, Suparna Patowary, Michael R. Stoneman, Julie A. Oliver, Liviu Movileanu and Valerică Raicu
The first critical review in Issue 2 comes from Vadim Mozhayskiy and Ilias Tagkopoulos at the UC Davis Genome Center, USA. They take a critical look at in vivo and in vitro methods of studying microbial evolution and this absorbing review was also highlighted on the IBiology blog very recently.
Microbial evolution in vivo and in silico: methods and applications
Vadim Mozhayskiy and Ilias Tagkopoulos
Viviany Taqueti and Farouc Jaffer at Harvard Medical School, USA, review the developments in using high-resolution molecular imaging and intravital microscopy to investigate vascular disease in their fascinating critical review, which was also highlighted on the blog this January.
High-resolution molecular imaging via intravital microscopy: illuminating vascular biology in vivo
Viviany R. Taqueti and Farouc A. Jaffer
Marco Cecchini et al. critically review how mechanotransduction affects cell biology, using the mechanical inputs that can regulate mesenchymal stromal cells as a case in point. MSCs have great potential in biomedical applications, including transplantation; therefore this review is of importance as it covers the controversy in the literature surrounding their characterisation and use in different tissues.
Endothelial differentiation of mesenchymal stromal cells: when traditional biology meets mechanotransduction
Orazio Vittorio, Emanuela Jacchetti, Simone Pacini and Marco Cecchini
Pedro Roda-Navarro at Universidad Complutense de Madrid, Spain summarises how microspectroscopy can now unveil the complexities of the molecular dynamics at the immunological synapse. This review also extends to how we can obtain quantitative information about the biochemical reactions in living cells, including quantitative imaging and theory.
Microspectroscopy reveals mechanisms of lymphocyte activation
There are of course plenty of innovative primary research articles in this month’s issue, including HOT articles such as:
Theoretical conditions for the stationary reproduction of model protocells
Fabio Mavelli and Kepa Ruiz-Mirazo