The combination of intravital microscopy (IVM) and fluorescence imaging is the focus of this Critical Review. IVM allows us to look at biological processes as they happen and fluorescence imaging gives advantages of subcellular-resolution and detection capability.
Viviany Taqueti and Farouc Jaffer at Harvard Medical School, USA, look at the advances in IVM technology and methods in this Critical Review, concentrating on its application in studying vascular disease. They include:
1. Agents for molecular specificity
2. Challenges of imaging vessel-based diseases atherosclerosis and thrombosis
3. Unique in vivo biological insights obtained using IVM
4. Brief comments on clinical application in the future
High-resolution molecular imaging via intravital microscopy: illuminating vascular biology in vivo
Viviany R. Taqueti and Farouc A. Jaffer