Collaborators in The Netherlands have created a 3D well system integrated into biomaterials that can be used either in vitro or in vivo for molecule screening in this HOT Technical Innovation.
The research was led by Gustavo Higuera and Jeanine Hendriks at Screvo Ltd and CellCoTec, with a team at The University of Twente. Avoiding animal experimentation by using 3D systems that mimic the in vivo environment is desirable yet challenging. Optimising animal experiments to be as efficient and effective as possible is a common goal of much research.
This team envisioned the integration of such in vitro 3D cell culture mimicking methods with biocompatible materials into animal models. To do this, they create a 9-well system that can be implanted into four sites under the skin of one mouse for in vivo screening. This enables testing of up to thirty six different conditions at once.
The innovative platform was produced from a wide range of biomaterials chosen for biocompatibility instead of conventional materials like PDMS. The cell culture compatibility of wells made with copolymer PEOT-PBT was tested using human mesenchymal stem cells. They test the systemic effect of the device on surrounding tissue of twenty mice.
This innovative bridge between in vivo and in vitro experimentation has great potential to both increase throughput and minimise animal experimentation by reducing the number of animals needed. Read the article in full now as it’s free to access for the next 4 weeks*:
In vivo screening of extracellular matrix components produced under multiple experimental conditions implanted in one animal
Gustavo A. Higuera, Jeanine A. A. Hendriks, Joost van Dalum, Ling Wu, Roka Schotel, Liliana Moreira-Teixeira, Mirella van den Doel, Jeroen C. H. Leijten, Jens Riesle, Marcel Karperien, Clemens A. van Blitterswijk and Lorenzo Moroni
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