Protein phosphorylation is an important post-translational modification that plays a vital role in cellular processes. It is necessary to separate phosphopeptides from complex mixtures and enrich the samples before mass spectrometry (MS) analysis. Xiuling Li, Xinmiao Liang and colleagues from the Dalian Institute of Chemical Physics, China, have developed a way to improve phosphopeptide enrichment efficiency, using a hydrophilic interaction liquid chromatography (HILIC) based material, cyclodextrin (CD) bonded silica (Click OEG-CD), which was synthesized and applied to phosphopeptide enrichment and fractionation.
Phosphopeptide enrichment and fractionation by using Click OEG-CD matrix
Yanyan Zhao, Xiuling Li, Jingyu Yan, Zhimou Guo and Xinmiao Liang
Anal. Methods, 2012, Advance Article
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