Being able to determine the concentration of the plasma protein human serum albumin (HSA) in biological media and pharmaceuticals is important because of its key role in various types of human medicine.
Frank Eertmans and colleagues from FertiPro N.V. in Belgium present a robust HPLC assay to accurately and quickly measure HSA in media used for assisted reproductive techniques. This method overcomes the inter-assay variabilities and accuracy issues of currently used colorimetric, electrophoretic and immunological assays.
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Development and validation of a high-performance liquid chromatography (HPLC) method for the determination of human serum albumin (HSA) in medical devices
Frank Eertmans, Veerle Bogaert and Barbara Puype
Anal. Methods, 2011