Archive for November, 2013

Immobilized phage proteins for detection of staphylococci

The rapid and specific detection of pathogenic bacteria, such as staphylococci, is an important worldwide concern. Staphylococci cause serious infections in humans and animals and certain species such as S. aureus and methicillin resistant S. aureus (MRSA) are developing antibiotic resistance that is quickly becoming a global crisis. The ability to detect these bacteria rapidly and in situ would allow for early treatment even in countries with poor access to healthcare.

Immobilized phage proteins for specific detection of staphylococci

Canadian researchers from McGill University and Polytechnique Montreal have recently shown the use of immobilized bacteriophage proteins to develop a biosensor for the specific detection of 8 clinical isolates of staphylococciHicham Chibli and coworkers examined several purified phage proteins from the Endolysin class of enzymes and exploited their ability to specifically bind staphylococci. Indeed these immobilized phage proteins did not bind to other closely related bacteria.

Bacteriophages have been used as recognition elements for biosensors before, but this research shows the use of specific phage proteins instead of whole bacteriophages. “Compared with whole phages, these single proteins are smaller, easier to purify, and easier to assemble in a reproducible manner of a surface” – says Jay Nadeau of McGill University – “The small size is key for development of some types of biosensors where sensitivity is dependent upon thickness of the functionalizing layer, for example, microresonators” – he adds. The ability to develop microresonators with specific detection can provide small, portable and specific biosensors for in field use.

To know more about this study, please access the link below. This paper will be free to read for the next three weeks.

Immobilized phage proteins for specific detection of staphylococci
Hicham Chibli, Hala Ghali, Soonhyang Park, Yves-Alain Peter and Jay L. Nadeau  
Analyst, 2014, 139, 179-186
DOI: 10.1039/C3AN01608K

Digg This
Reddit This
Stumble Now!
Share on Facebook
Bookmark this on Delicious
Share on LinkedIn
Bookmark this on Technorati
Post on Twitter
Google Buzz (aka. Google Reader)

HOT Articles in Analyst

Take a look at our new HOT articles just published in Analyst and free for you for the next couple of weeks:

Development of coated-wire silver ion selective electrodes on paper using conductive films of silver nanoparticles
Wanwisa Janrungroatsakul, Chutiparn Lertvachirapaiboon, Wittaya Ngeontae, Wanlapa Aeungmaitrepirom, Orawon Chailapakul, Sanong Ekgasitb and Thawatchai Tuntulani
Analyst, 2013,138, 6786-6792
DOI: 10.1039/C3AN01385E

A visible light photoelectrochemical sensor for tumor marker detection using tin dioxide quantum dot–graphene as labels
Yanhu Wang, Meng Li, Yuanna Zhu, Shenguang Ge, Jinghua Yu, Mei Yan and Xianrang Song
Analyst, 2013,138, 7112-7118
DOI: 10.1039/C3AN01410J

Retention in continuous two-dimensional thermal field-flow fractionation: comparison of experimental results with theory
Pertti Vastamäki, P. Stephen Williams, Matti Jussila, Michel Martin and Marja-Liisa Riekkola
Analyst, 2014, Advance Article
DOI: 10.1039/C3AN01047C

Cascade signal amplification for ultrasensitive electrochemical DNA detection
Jin Xu, Qiong Wang, Yun Xiang, Ruo Yuan and Yaqin Chai
Analyst, 2014, Advance Article
DOI: 10.1039/C3AN01673K

Detection and characterization of silver nanoparticles and dissolved species of silver in culture medium and cells by AsFlFFF-UV-Vis-ICPMS: application to nanotoxicity tests
E. Bolea, J. Jiménez-Lamana, F. Laborda, I. Abad-Álvaro, C. Bladé, L. Arola and J. R. Castillo
Analyst, 2014, Advance Article
DOI: 10.1039/C3AN01443F

MALDI mechanisms: wavelength and matrix dependence of the coupled photophysical and chemical dynamics model
Richard Knochenmuss
Analyst, 2014, Advance Article
DOI: 10.1039/C3AN01446K

Target-induced quenching for highly sensitive detection of nucleic acids based on label-free luminescent supersandwich DNA/silver nanoclusters
Guangfeng Wang, Yanhong Zhu, Ling Chen, Lun Wang and Xiaojun Zhang
Analyst, 2014, Advance Article
DOI: 10.1039/C3AN01702H

LED-based interferometric reflectance imaging sensor for the detection of amyloid-β aggregation
Xin R. Cheng, George G. Daaboul, M. Selim Ünlü and Kagan Kerman
Analyst, 2014, Advance Article
DOI: 10.1039/C3AN01307C

Improved accuracy for label-free absolute quantification of proteome by combining the absolute protein expression profiling algorithm and summed tandem mass spectrometric total ion current
Qi Wu, Yichu Shan, Yanyan Qu, Hao Jiang, Huiming Yuan, Jianxi Liu, Shen Zhang, Zhen Liang, Lihua Zhang and Yukui Zhang
Analyst, 2014, Advance Article
DOI: 10.1039/C3AN01738A

Focussed ion beam serial sectioning and imaging of monolithic materials for 3D reconstruction and morphological parameter evaluation
Mercedes Vázquez, David Moore, Xiaoyun He, Aymen Ben Azouz, Ekaterina Nesterenko, Pavel Nesterenko, Brett Paull and Dermot Brabazon
Analyst, 2014, Advance Article
DOI: 10.1039/C3AN01827J

Digg This
Reddit This
Stumble Now!
Share on Facebook
Bookmark this on Delicious
Share on LinkedIn
Bookmark this on Technorati
Post on Twitter
Google Buzz (aka. Google Reader)

Therapeutic screening for Alzheimer’s disease

Scientists in Canada and the US hope a system they have developed for monitoring amyloid-β aggregation on a chip could be used to find new treatments for Alzheimer’s disease.

Alzheimer’s disease is a complex neurodegenerative condition and the most common cause of dementia. In the US, more than 5 million people are estimated to have the condition. There is currently no known cure. Research has uncovered that the self-aggregation of the amyloid-β (Aβ) peptide plays a vital role in the development of Alzheimer’s disease. Methods that study the interaction of Aβ with potential new drugs are therefore extremely important.

Now, Kagan Kerman at the University of Toronto at Scarborough, and colleagues, have created a sensing platform to aid the drug screening process. ‘We have successfully demonstrated a novel method for high throughput screening of small molecules modulating Aβ growth and it provides a promising platform to facilitate therapeutics discovery for Alzheimer’s disease,’ says Kerman.

To read the full article, please visit Chemistry World.

LED-based interferometric reflectance imaging sensor for the detection of amyloid-β aggregation
Xin R. Cheng, George G. Daaboul, M. Selim Ünlü and Kagan Kerman
Analyst, 2014, Advance Article
DOI: 10.1039/C3AN01307C, Paper

Digg This
Reddit This
Stumble Now!
Share on Facebook
Bookmark this on Delicious
Share on LinkedIn
Bookmark this on Technorati
Post on Twitter
Google Buzz (aka. Google Reader)

Analysis of thyroid tissues by FT-IR microscopectroscopy

 

FTIR spectra of hormones triiodothyronine, thyroxine, diiodotyrosine, tyrosine and thyroid tissue

The combination of vibrational spectroscopy with mapping and imaging techniques to address important biochemical questions is an area of active and expanding research.  By subjecting adjacent tissue sections to standard histopathological screening and spectroscopic imaging, a greater understanding of the biochemical processes underlying tissue form and function can be achieved.

Researchers from Northeastern University, USA, and the Institute of Energy and Nuclear Research (IPEN), Sao Paolo, have used Fourier transform infrared (FTIR) microspectroscopy to examine healthy thyroid tissues. FTIR allows spatially resolved images, or maps, to be obtained by use of focal plane array detectors and spectral processing of the data then reveals chemical images.  In this HOT Analyst paper, Denise Zezell and coworkers present an example from their investigation of 80 different patient samples, which were analysed by transflection-mode FTIR mapping.  The approach was  applied to the study of healthy thyroid tissue and, with reference to thyroid specific hormones, iodination state. 

To know more about this research, click on the links below. This paper will be free to read for the next three weeks:

The characterization of normal thyroid tissue by micro-FTIR spectroscopy
Thiago M. Pereira, Denise M. Zezell, Benjamin Bird, Milos Miljković and Max Diem
Analyst, 2013,138, 7094-7100
DOI: 10.1039/C3AN00296A

Digg This
Reddit This
Stumble Now!
Share on Facebook
Bookmark this on Delicious
Share on LinkedIn
Bookmark this on Technorati
Post on Twitter
Google Buzz (aka. Google Reader)

Classifying myobacterial strains using surface enhanced Raman spectroscopy

Spectroscopic analysis of mycolic acid profiles

In bacterial-based diagnostics, the bacteria can evolve over time and it is important to be able to differentiate between multiple strains. Mycobacterium tuberculosis, which is responsible for causing tuberculosis (TB), has multiple drug-resistant strains. The most accurate method to identify toxic strains of TB, high performance liquid chromatography (HPLC), requires fluorescent labeling and extraction of the mycolic acids, from the outer membrane of the bacteria.

Researchers at the University of Georgia in the United States wanted a more direct detection method and approached this problem in two important aspects. They began by identifying the high molecular weight lipids within the bacteria using HPLC, gas chromatography (GC), and nuclear magnetic resonance (NMR) for several TB bacterial strains. NMR in particular was very important in identifying the structures of mycolic acids. Using the information from these techniques and surface enhanced Raman spectroscopy (SERS), they chemically classified the mycolic acid from both TB and non-TB strains. The SERS spectra underwent further statistical analysis and accurately identified different strains of TB label-free and with high sensitivity.

To know more about this research, click on the link below. This paper will be free to read for the next three weeks.

Identification of mycobacteria based on spectroscopic analyses of mycolic acid profiles
Omar E. Rivera-Betancourt, Russell Karls, Benjamin Grosse-Siestrup, Shelly Helms, Frederick Quinn and Richard A. Dluhy
Analyst, 2013,138, 6774-6785
DOI: 10.1039/C3AN01157G

Digg This
Reddit This
Stumble Now!
Share on Facebook
Bookmark this on Delicious
Share on LinkedIn
Bookmark this on Technorati
Post on Twitter
Google Buzz (aka. Google Reader)

Probe and Chip Approaches to Cell Analysis Themed Issue: Submissions Open!

Harris et al., Analyst, 2013, 138, 4769-4776

We are delighted to announce a new themed issue of Analyst on

Probe and Chip Approaches to Cell Analysis

This issue, edited by Professor Luke Lee (University of California, Berkeley, USA) and Professor Jonathan Cooper (University of Glasgow, UK), aims to cover a wide range of aspects of cell analysis based on recent microfluidic and lab-on-a chip technologies by highlighting analytical developments and novel applications.

Why not submit today? Submission deadline: 9th January 2014. Email us at ANALYST-RSC@rsc.org for more information.

All articles will be peer-reviewed and, to be suitable for publication, must meet the usual quality and significance standards of the journal.

Please indicate in your covering letter that your article is to be considered for the Probe and Chip Approaches to Cell Analysis themed issue.

Digg This
Reddit This
Stumble Now!
Share on Facebook
Bookmark this on Delicious
Share on LinkedIn
Bookmark this on Technorati
Post on Twitter
Google Buzz (aka. Google Reader)

More HOT Articles in Analyst

Take a look at our new HOT articles just published in Analyst! These papers are all free to read for the next three weeks. Enjoy!

In vitro detection of calcium in bone by modified carbon dots
A. Shanti Krishna, C. Radhakumary and K. Sreenivasan
Analyst, 2013,138, 7107-7111
DOI: 10.1039/C3AN01239E, Paper

Measuring and modelling cell-to-cell variation in uptake of gold nanoparticles
J. Charles. G. Jeynes, Christopher Jeynes, Michael J. Merchant and Karen J. Kirkby
Analyst, 2013,138, 7070-7074
DOI: 10.1039/C3AN01406A, Communication

Adenosine capped QDs based fluorescent sensor for detection of dopamine with high selectivity and sensitivity
Qin Mu, Hu Xu, Yan Li, Shijian Ma and Xinhua Zhong
Analyst, 2013, Advance Article
DOI: 10.1039/C3AN01592K, Paper

A pyrene-based simple but highly selective fluorescence sensor for Cu2+ ions via a static excimer mechanism
Soma Sarkar, Swapnadip Roy, Anindita Sikdar, R. N. Saha and Sujit S. Panja
Analyst, 2013,138, 7119-7126
DOI: 10.1039/C3AN00928A, Paper

Whole organ cross-section chemical imaging using label-free mega-mosaic FTIR microscopy
Paul Bassan, Ashwin Sachdeva, Jonathan H. Shanks, Mick D. Brown, Noel W. Clarke and Peter Gardner
Analyst, 2013,138, 7066-7069
DOI: 10.1039/C3AN01674A, Communication

A highly sensitive LED-induced chemiluminescence platform for aptasensing of platelet-derived growth factor
Xinfeng Zhang, Hui Zhang, Shuxia Xu and Yonghua Sun
Analyst, 2013, Advance Article
DOI: 10.1039/C3AN01566A, Paper

Effects of surface charges of graphene oxide on neuronal outgrowth and branching
Qin Tu, Long Pang, Yun Chen, Yanrong Zhang, Rui Zhang, Bingzhang Lu and Jinyi Wang
Analyst, 2013, Advance Article
DOI: 10.1039/C3AN01796F, Paper

Paracrine signalling of inflammatory cytokines from an in vitro blood brain barrier model upon exposure to polymeric nanoparticles
Michelle Nic Raghnaill, Mattia Bramini, Dong Ye, Pierre-Olivier Couraud, Ignacio A. Romero, Babette Weksler, Christoffer Åberg, Anna Salvati, Iseult Lynch and Kenneth A Dawson
Analyst, 2013, Accepted Manuscript
DOI: 10.1039/C3AN01621H, Paper

Adenosine capped QDs based fluorescent sensor for detection of dopamine with high selectivity and sensitivity
Qin Mu, Hu Xu, Yan Li, Shijian Ma and Xinhua Zhong
Analyst, 2013, Advance Article
DOI: 10.1039/C3AN01592K, Paper

Digg This
Reddit This
Stumble Now!
Share on Facebook
Bookmark this on Delicious
Share on LinkedIn
Bookmark this on Technorati
Post on Twitter
Google Buzz (aka. Google Reader)

HOT Articles in Analyst

Take a look at our new HOT articles just published in Analyst and free for you for the next couple of weeks:

Combined in situ atomic force microscopy and infrared attenuated total reflection spectroelectrochemistry
Daniel Neubauer, Jochen Scharpf, Alberto Pasquarelli, Boris Mizaikoff and Christine Kranz
Analyst, 2013,138, 6746-6752
DOI: 10.1039/C3AN01169K, Paper

An iridium(III)-based lab-on-a-molecule for cysteine/homocysteine and tryptophan using triple-channel interrogation Kun Chen and Michael Schmittel
Analyst, 2013,138, 6742-6745
DOI: 10.1039/C3AN01530K, Communication

Determination of aerosol oxidative activity using silver nanoparticle aggregation on paper-based analytical devices
Wijitar Dungchai, Yupaporn Sameenoi, Orawon Chailapakul, John Volckens and Charles S. Henry
Analyst, 2013,138, 6766-6773
DOI: 10.1039/C3AN01235B, Paper

Biocatalytic analysis of biomarkers for forensic identification of ethnicity between Caucasian and African American groups
Friederike Kramer, Lenka Halámková, Arshak Poghossian, Michael J. Schöning, Evgeny Katz and Jan Halámek
Analyst, 2013,138, 6251-6257
DOI: 10.1039/C3AN01062G, Communication

Biocatalytic analysis of biomarkers for forensic identification of ethnicity between Caucasian and African American groups
Friederike Kramer, Lenka Halámková, Arshak Poghossian, Michael J. Schöning, Evgeny Katz and Jan Halámek
Analyst, 2013,138, 6251-6257
DOI: 10.1039/C3AN01062G, Communication

Analysis of free fractions for chiral drugs using ultrafast extraction and multi-dimensional high-performance affinity chromatography
Xiwei Zheng, Michelle J. Yoo and David S. Hage
Analyst, 2013,138, 6262-6265
DOI: 10.1039/C3AN01315D, Communication

Radical cascades in electron transfer dissociation (ETD) – implications for characterizing peptide disulfide regio-isomers
Lei Tan, Kirt L. Durand, Xiaoxiao Ma and Yu Xia
Analyst, 2013,138, 6759-6765
DOI: 10.1039/C3AN01333B, Paper

Enhanced peroxydisulfate electrochemiluminescence for dopamine biosensing based on Au nanoparticle decorated reduced graphene oxide
Yuting Yan, Qian Liu, Kun Wang, Ling Jiang, Xingwang Yang, Jing Qian, Xiaoya Dong and Baijing Qiu
Analyst, 2013,138, 7101-7106
DOI: 10.1039/C3AN01533E, Paper

Digg This
Reddit This
Stumble Now!
Share on Facebook
Bookmark this on Delicious
Share on LinkedIn
Bookmark this on Technorati
Post on Twitter
Google Buzz (aka. Google Reader)

Featured on the Covers of Issue 23

Stephenson-Brown et al., Analyst, 2013, 138, 7146-7151

We are delighted to highlight the latest covers of Analyst, showing new research on the development and application of a sensory system to detect glucose.

John Fossey from the University Birmingham, UK, and collaborators at the University of Bath, UK, and at the East China University of Science and Technology, China, present two papers describing a new glucose sensor based on the principle of self-assembly and surface plasmon resonance (SPR) optical technique.

Wang et al., Analyst, 2013, 138, 7146-7151

The sensor is prepared modifying the surface of a gold electrode with a bis-boronic acid receptor. The receptor contains a glucose selective chemosensor unit and a surface anchoring unit, which allows selective detection of glucose. This system has the unique capacity to label-free and allows detection of molecular reactions in real time with highly sensitivity. According to this research, SPR could be an attractive sensing platform to monitor glucose and possibly other analytes.

To read more about this work, please access the links below. These two papers are free to read, so have a look now:

A bis-boronic acid modified electrode for the sensitive and selective determination of glucose concentrations
Hui-Chen Wang, Hao Zhou, Baoqin Chen, Paula M. Mendes, John S. Fossey, Tony D. James and   Yi-Tao Long
Analyst, 2013, 138, 7146-7151
DOI: 10.1039/C3AN01234D

Glucose selective Surface Plasmon Resonance-based bis-boronic acid sensor
Alex Stephenson-Brown, Hui-Chen Wang, Parvez Iqbal, Jon A. Preece, Yitao Long, John S. Fossey, Tony D. James and   Paula M. Mendes 
Analyst, 2013, 138, 7140-7145
DOI: 10.1039/C3AN01233F

Digg This
Reddit This
Stumble Now!
Share on Facebook
Bookmark this on Delicious
Share on LinkedIn
Bookmark this on Technorati
Post on Twitter
Google Buzz (aka. Google Reader)

Top ten most accessed Analyst articles from July to September 2013

Here are the top ten most accessed Analyst articles for July, August and September 2013.

Detection of calcium phosphate crystals in the joint fluid of patients with osteoarthritis – analytical approaches and challenges
Alexander Yavorskyy, Aaron Hernandez-Santana, Geraldine McCarthy and Gillian McMahon  
Analyst, 2008,133, 302-318
DOI: 10.1039/b716791a

Illuminating disease and enlightening biomedicine: Raman spectroscopy as a diagnostic tool
David I. Ellis, David P. Cowcher, Lorna Ashton, Steve O’Hagan and Royston Goodacre  
Analyst, 2013,138, 3871-3884
DOI: 10.1039/c3an00698k

Upconversion nanoparticles in biological labeling, imaging, and therapy
Feng Wang, Debapriya Banerjee, Yongsheng Liu, Xueyuan Chen and Xiaogang Liu
Analyst, 2010,135, 1839-1854
DOI: 10.1039/c0an00144a

Advances in nano-scaled biosensors for biomedical applications
Jianling Wang, Guihua Chen, Hui Jiang, Zhiyong Li and Xuemei Wang  
Analyst, 2013,138, 4427-4435
DOI: 10.1039/c3an00438d

A coumarin–indole-based near-infrared ratiometric pH probe for intracellular fluorescence imaging
Xiao-Dong Liu, Ying Xu, Ru Sun, Yu-Jie Xu, Jian-Mei Lu and Jian-Feng Ge  
Analyst, 2013,138, 6542-6550
DOI: 10.1039/c3an01033c

Analytical methods to assess nanoparticle toxicity
Bryce J. Marquis, Sara A. Love, Katherine L. Braun and Christy L. Haynes  
Analyst, 2009,134, 425-439
DOI: 10.1039/b818082b

Solid-state Forensic Finger sensor for integrated sampling and detection of gunshot residue and explosives: towards ‘Lab-on-a-finger’
Amay J. Bandodkar, Aoife M. O’Mahony, Julian Ramírez, Izabela A. Samek, Sean M. Anderson, Joshua R. Windmiller and Joseph Wang
Analyst, 2013,138, 5288-5295
DOI: 10.1039/c3an01179h

Selective fluorescence sensing of Cu(ii) and Zn(ii) using a new Schiff base-derived model compound: naked eye detection and spectral deciphering of the mechanism of sensory action
Aniruddha Ganguly, Bijan Kumar Paul, Soumen Ghosh, Samiran Kar and Nikhil Guchhait
Analyst, 2013,138, 6532-6541
DOI: 10.1039/c3an00155e

Carbon dots and chitosan composite film based biosensor for the sensitive and selective determination of dopamine
Qitong Huang, Shirong Hu, Hanqiang Zhang, Jianhua Chen, Yasan He, Feiming Li, Wen Weng, Jiancong Ni, Xiuxiu Bao and Yi Lin  
Analyst, 2013,138, 5417-5423
DOI: 10.1039/c3an00510k

Quantum dots for fluorescent biosensing and bio-imaging applications
Jingjing Li and Jun-Jie Zhu  
Analyst, 2013,138, 2506-2515
DOI: 10.1039/c3an36705c

We hope you enjoyed reading these articles, and if you have any thoughts or comments, please leave these in the comment box below.

Fancy submitting an article to Analyst? Why not submit to us here today or alternatively email us your suggestions!

Digg This
Reddit This
Stumble Now!
Share on Facebook
Bookmark this on Delicious
Share on LinkedIn
Bookmark this on Technorati
Post on Twitter
Google Buzz (aka. Google Reader)