Archive for August, 2012

HOT Article: A facile graphene oxide-based DNA polymerase assay

Wang et al., Analyst, 2012, 137, 3989

By Laura Ruvuna, Web Writer

In this HOT article, Analyst Advisory Board member Kemin Wang and co-workers from Hunan University, China, use graphene oxide acting as an optical switch in a DNA polymerase assay for the first time. DNA polymerase is important in molecular biological studies and functions as a key drug target. Typically, these assays use expensive and restrictive radioactive labels, which are unsuitable for a high throughput system. Unlike other fluorescence-based assays that require designed template sequences or expensive reagents, here the graphene oxide quenches the fluorescently labeled ssDNA. When the complementary strand binds, the DNA has less of an affinity for the graphene and the resulting fluorescence signal increases. To read more about this new and exciting assay, click the link below. It will be free to read for 2 weeks.

A facile graphene oxide-based DNA polymerase assay
Fengzhou Xu, Hui Shi, Xiaoxiao He, Kemin Wang, Xiaosheng Ye, Lv’an Yan and Shuyong Wei
Analyst, 2012, 137, 3989-3994
DOI: 10.1039/C2AN35585J

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Top ten most accessed articles in June

This month sees the following articles in Analyst that are in the top ten most accessed:-

Easy Dual-Mode Ambient Mass Spectrometry with Venturi Self-Pumping, Canned Air, Disposable Parts and Voltage-Free Sonic-Spray Ionization
Nicolas V. Schwab ,  Andreia M. Porcari ,  Mirela B. Coelho ,  Eduardo M. Schmidt ,  Jose L. Jara ,  Jesui V. Visentainer and Marcos N. Eberlin
Analyst, 2012,137, 2537-2540 DOI: 10.1039/C2AN16312H  

   
A Ratiometric Fluorescent Chemosensor for Iron: Discrimination of Fe2+ and Fe3+ and Living Cell Application 
Supriti Sen ,  Sandipan Sarkar ,  Basab Chattopadhyay ,  Anuradha Moirangthem ,  Anupam Basu ,  Koushik Dhara and Pabitra Chattopadhyay 
Analyst, 2012,137, 3335-3342 DOI: 10.1039/C2AN35258C     

Ultrasensitive Colorimetric Detection of Heparin Based on Self-assembly of Gold Nanoparticles on Graphene Oxide 
Xiuli Fu ,  Lingxin Chen and Jinhua Li  
Analyst, 2012,137, 3653-3658 DOI: 10.1039/C2AN35552C     

A new fluorescent and colorimetric probe for Cu2+ in live cells 
Wei-Yong Liu ,  Hai-Ying Li ,  Bao-Xiang Zhao and Jun-Ying Miao  
Analyst, 2012,137, 3466-3469 DOI: 10.1039/C2AN35559K     

A colorimetric sensor based on catechol-terminated mixed self-assembled monolayers modified gold nanoparticles for ultrasensitive detections of copper ions 
Siqiu Ye ,  Xinhao Shi ,  Wei Gu ,  Yixuan Zhang and Yuezhong Xian 
Analyst, 2012,137, 3365-3371 DOI: 10.1039/C2AN35311C    

Upconversion Nanoparticles in Biological Labeling, Imaging, and Therapy 
Feng Wang ,  Debapriya Banerjee ,  Yongsheng Liu ,  Xueyuan Chen and Xiaogang Liu  
Analyst, 2010,135, 1839-1854 DOI: 10.1039/C0AN00144A     

Bacteriophage based probes for pathogen detection 
Amit Singh ,  Denis Arutyunov ,  Christine M. Szymanski and Stephane Evoy  
Analyst, 2012,137, 3405-3421 DOI: 10.1039/C2AN35371G     

A sensitive dual signal amplification method in western blotting based on antibody-functionalised graphene oxide and gold nanoparticles 
Hongjun Lin ,  Jingrui Huo ,  Aihong Zhang ,  Yingfu Liu ,  Qingming Wang ,  Yun Cai ,  Wantao Ying ,  Weijie Qin ,  Yangjun Zhang and Xiaohong Qian  
Analyst, 2012,137, 3620-3623 DOI: 10.1039/C2AN35242G    

Micro- and nanostructures and their application in gas chromatography 
M. Mittermüller and D. A. Volmer  
Analyst, 2012,137, 3195-3201 DOI: 10.1039/C2AN35184F     

A Fluorescent Probe Directly Detect Peroxynitrite Based on Boronate Oxidation and Its Applications for Fluorescence Imaging in Living Cells 
Fabiao Yu ,  Ping Song ,  Peng Li ,  Bingshuai Wang and Keli Han  
Analyst, 2012,137, 3740-3749 DOI: 10.1039/C2AN35246J     

Why not take a look at the articles today and blog your thoughts and comments below.

Fancy submitting an article to Analyst? Then why not submit to us today or alternatively email us  your suggestions.

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HOT articles in Analyst for July

Check out some of the HOT articles published last month in Analyst that you may have missed. These papers will be free to access for 2 weeks!

Analyst

13C cell wall enrichment and ionic liquid NMR analysis: progress towards a high-throughput detailed chemical analysis of the whole plant cell wall
Marcus Foston, Reichel Samuel and Arthur J. Ragauskas
Analyst, 2012, 137, 3904-3909
DOI: 10.1039/C2AN35344J

Transmission Raman measurement directly through packed corn kernels to improve sample representation and accuracy of compositional analysis
Kayeong Shin, Hoeil Chung and Chul-won Kwak
Analyst, 2012, 137, 3690-3696
DOI: 10.1039/C2AN35443H

A water soluble Al3+ selective colorimetric and fluorescent turn-on chemosensor and its application in living cell imaging
Supriti Sen, Titas Mukherjee, Basab Chattopadhyay, Anuradha Moirangthem, Anupam Basu, Jaromir Marek and Pabitra Chattopadhyay
Analyst, 2012, 137, 3975-3981
DOI: 10.1039/C2AN35560D

Silylated BODIPY dyes and their use in dye-encapsulated silica nanoparticles with switchable emitting wavelengths for cellular imaging
Xuehua Hong, Zhuyuan Wang, Jing Yang, Qingdong Zheng, Shenfei Zong, Yu Sheng, Deqin Zhu, Changquan Tang and Yiping Cui
Analyst, 2012, Advance Article
DOI: 10.1039/C2AN35389J

Fourier transform infrared imaging analysis in discrimination studies of squamous cell carcinoma
J. D. Pallua, C. Pezzei, B. Zelger, G. Schaefer, L. K. Bittner, V. A. Huck-Pezzei, S. A. Schoenbichler, H. Hahn, A. Kloss-Brandstaetter, F. Kloss, G. K. Bonn and C. W. Huck
Analyst, 2012, 137, 3965-3974
DOI: 10.1039/C2AN35483G

Fabrication of glutathione photoelectrochemical biosensor using graphene–CdS nanocomposites
Xiaomei Zhao, Shiwei Zhou, Qingming Shen, Li-Ping Jiang and Jun-Jie Zhu
Analyst, 2012, 137, 3697-3703
DOI: 10.1039/C2AN35658A

BODIPY based colorimetric fluorescent probe for selective thiophenol detection: theoretical and experimental studies
Dnyaneshwar Kand, Pratyush Kumar Mishra, Tanmoy Saha, Mayurika Lahiri and Pinaki Talukdar
Analyst, 2012, 137, 3921-3924
DOI: 10.1039/C2AN35524H

Quantification of low-polar small molecules using room temperature ionic liquids matrix-assisted desorption corona beam ionization
Hua Wang, Yongning Wu, Bin Guo, Wenjian Sun, Li Ding and Bo Chen
Analyst, 2012, 137, 3982-3988
DOI: 10.1039/C2AN35508F

Silver nanoparticle aggregates on copper foil for reliable quantitative SERS analysis of polycyclic aromatic hydrocarbons with a portable Raman spectrometer
Xiaohong Jiang, Yongchao Lai, Min Yang, Heng Yang, Wei Jiang and Jinhua Zhan
Analyst, 2012, 137, 3995-4000
DOI: 10.1039/C2AN35713E

A BODIPY-functionalized bimetallic probe for sensitive and selective color-fluorometric chemosensing of Hg2+
Hyunjong Son, Ji Ha Lee, Yang-Rae Kim, In Su Lee, Sanyang Han, Xiaogang Liu, Justyn Jaworski and Jong Hwa Jung
Analyst, 2012, 137, 3914-3916
DOI: 10.1039/C2AN35704F

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HOT articles in Issue 17

BODIPY-functionalized bimetallic probe for sensitive and selective color-fluorometric chemosensing of mercury

Son et al., Analyst, 2012, 137, 3914-3916

As well as the cover articles, we have a good helping of HOT articles for you in our latest issue.

These include a paper from Advisory Board member Kemin Wang and colleagues on a novel, simple and cost-effective fluorometric DNA polymerase detection method which utilizes graphene oxide as a signal switch.

There’s also a new BODIPY dye conjugate for selective quenching of mercury (see the colourful graphical abstract to the left) from researchers in Korea and Singapore.

Take a look at all the HOTs below – free to access for the next 2 weeks.

(more…)

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