By Laura Ruvuna, Web Writer
In this HOT article, Analyst Advisory Board member Kemin Wang and co-workers from Hunan University, China, use graphene oxide acting as an optical switch in a DNA polymerase assay for the first time. DNA polymerase is important in molecular biological studies and functions as a key drug target. Typically, these assays use expensive and restrictive radioactive labels, which are unsuitable for a high throughput system. Unlike other fluorescence-based assays that require designed template sequences or expensive reagents, here the graphene oxide quenches the fluorescently labeled ssDNA. When the complementary strand binds, the DNA has less of an affinity for the graphene and the resulting fluorescence signal increases. To read more about this new and exciting assay, click the link below. It will be free to read for 2 weeks.
A facile graphene oxide-based DNA polymerase assay
Fengzhou Xu, Hui Shi, Xiaoxiao He, Kemin Wang, Xiaosheng Ye, Lv’an Yan and Shuyong Wei
Analyst, 2012, 137, 3989-3994